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Selection of reference genes for normalization of quantitative real-time PCR in organ culture of the rat and rabbit intervertebral disc
BACKGROUND: The accuracy of quantitative real-time RT-PCR (qRT-PCR) is often influenced by experimental artifacts, resulting in erroneous expression profiles of target genes. The practice of employing normalization using a reference gene significantly improves reliability and its applicability to mo...
Autores principales: | Seol, Dongrim, Choe, Hyeonghun, Zheng, Hongjun, Jang, Keewoong, Ramakrishnan, Prem S, Lim, Tae-Hong, Martin, James A |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3118343/ https://www.ncbi.nlm.nih.gov/pubmed/21615931 http://dx.doi.org/10.1186/1756-0500-4-162 |
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