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Modulation and Interaction of Immune-Associated Parameters with Antioxidant in the Immunocytes of Crab Scylla paramamosain Challenged with Lipopolysaccharides

Invertebrates are dependent on cellular and humoral immune defences against microbial infection. Scylla paramamosain is an important commercial species, but the fundamental knowledge on its immune defense related to the antioxidant and immune-associated reactions is still lacking. The study was to d...

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Autores principales: Gopalakrishnan, Singaram, Chen, Fang-Yi, Thilagam, Harikrishnan, Qiao, Kun, Xu, Wan-Fang, Wang, Ke-Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3118543/
https://www.ncbi.nlm.nih.gov/pubmed/21716691
http://dx.doi.org/10.1155/2011/824962
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author Gopalakrishnan, Singaram
Chen, Fang-Yi
Thilagam, Harikrishnan
Qiao, Kun
Xu, Wan-Fang
Wang, Ke-Jian
author_facet Gopalakrishnan, Singaram
Chen, Fang-Yi
Thilagam, Harikrishnan
Qiao, Kun
Xu, Wan-Fang
Wang, Ke-Jian
author_sort Gopalakrishnan, Singaram
collection PubMed
description Invertebrates are dependent on cellular and humoral immune defences against microbial infection. Scylla paramamosain is an important commercial species, but the fundamental knowledge on its immune defense related to the antioxidant and immune-associated reactions is still lacking. The study was to differentiate the responses of immune-associated parameters of haemolymph components in S. paramamosain when challenged with bacterial lipopolysaccharides (LPSs). The immunostimulating effects of LPS in crab by triggering various immune parameters (phagocytosis, lysozyme, antibacterial activity, phenoloxidase, and the generation of superoxide and nitric oxide) were investigated. Results showed that the generation of free radicals, phenoloxidase, lysozyme and antibacterial activities was significantly increased through the exposure periods. Conversely, total hemocyte count and lysosomal membrane stability decreased significantly as the exposure period extended to 96 h. The relationship between the antioxidant enzymes and immune reactions due to LPS was highly significant. In addition, ROS production was positively correlated with antioxidant showing immediate response of antioxidant defense to the oxyradicals generated. Overall, the study indicated that nonspecific immune components in hemocytes of crab showed active response to the LPS stimulation, and their responses suggested that many immune-associated parameters could be modulated and interrelated with the influence of antioxidants in crustaceans.
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spelling pubmed-31185432011-06-28 Modulation and Interaction of Immune-Associated Parameters with Antioxidant in the Immunocytes of Crab Scylla paramamosain Challenged with Lipopolysaccharides Gopalakrishnan, Singaram Chen, Fang-Yi Thilagam, Harikrishnan Qiao, Kun Xu, Wan-Fang Wang, Ke-Jian Evid Based Complement Alternat Med Research Article Invertebrates are dependent on cellular and humoral immune defences against microbial infection. Scylla paramamosain is an important commercial species, but the fundamental knowledge on its immune defense related to the antioxidant and immune-associated reactions is still lacking. The study was to differentiate the responses of immune-associated parameters of haemolymph components in S. paramamosain when challenged with bacterial lipopolysaccharides (LPSs). The immunostimulating effects of LPS in crab by triggering various immune parameters (phagocytosis, lysozyme, antibacterial activity, phenoloxidase, and the generation of superoxide and nitric oxide) were investigated. Results showed that the generation of free radicals, phenoloxidase, lysozyme and antibacterial activities was significantly increased through the exposure periods. Conversely, total hemocyte count and lysosomal membrane stability decreased significantly as the exposure period extended to 96 h. The relationship between the antioxidant enzymes and immune reactions due to LPS was highly significant. In addition, ROS production was positively correlated with antioxidant showing immediate response of antioxidant defense to the oxyradicals generated. Overall, the study indicated that nonspecific immune components in hemocytes of crab showed active response to the LPS stimulation, and their responses suggested that many immune-associated parameters could be modulated and interrelated with the influence of antioxidants in crustaceans. Hindawi Publishing Corporation 2011 2011-06-05 /pmc/articles/PMC3118543/ /pubmed/21716691 http://dx.doi.org/10.1155/2011/824962 Text en Copyright © 2011 Singaram Gopalakrishnan et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gopalakrishnan, Singaram
Chen, Fang-Yi
Thilagam, Harikrishnan
Qiao, Kun
Xu, Wan-Fang
Wang, Ke-Jian
Modulation and Interaction of Immune-Associated Parameters with Antioxidant in the Immunocytes of Crab Scylla paramamosain Challenged with Lipopolysaccharides
title Modulation and Interaction of Immune-Associated Parameters with Antioxidant in the Immunocytes of Crab Scylla paramamosain Challenged with Lipopolysaccharides
title_full Modulation and Interaction of Immune-Associated Parameters with Antioxidant in the Immunocytes of Crab Scylla paramamosain Challenged with Lipopolysaccharides
title_fullStr Modulation and Interaction of Immune-Associated Parameters with Antioxidant in the Immunocytes of Crab Scylla paramamosain Challenged with Lipopolysaccharides
title_full_unstemmed Modulation and Interaction of Immune-Associated Parameters with Antioxidant in the Immunocytes of Crab Scylla paramamosain Challenged with Lipopolysaccharides
title_short Modulation and Interaction of Immune-Associated Parameters with Antioxidant in the Immunocytes of Crab Scylla paramamosain Challenged with Lipopolysaccharides
title_sort modulation and interaction of immune-associated parameters with antioxidant in the immunocytes of crab scylla paramamosain challenged with lipopolysaccharides
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3118543/
https://www.ncbi.nlm.nih.gov/pubmed/21716691
http://dx.doi.org/10.1155/2011/824962
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