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Genetic examination of the putative skull of Jan Kochanowski reveals its female sex

We report the results of genetic examination of the putative skull of Jan Kochanowski (1530-1584), a great Polish renaissance poet. The skull was retrieved in 1791 by historian Tadeusz Czacki from the Kochanowski family tomb and became the property of the Czartoryskis Museum in Krakow. An anthropolo...

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Autores principales: Kupiec, Tomasz, Branicki, Wojciech
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Croatian Medical Schools 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3118727/
https://www.ncbi.nlm.nih.gov/pubmed/21674838
http://dx.doi.org/10.3325/cmj.2011.52.403
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author Kupiec, Tomasz
Branicki, Wojciech
author_facet Kupiec, Tomasz
Branicki, Wojciech
author_sort Kupiec, Tomasz
collection PubMed
description We report the results of genetic examination of the putative skull of Jan Kochanowski (1530-1584), a great Polish renaissance poet. The skull was retrieved in 1791 by historian Tadeusz Czacki from the Kochanowski family tomb and became the property of the Czartoryskis Museum in Krakow. An anthropological study in 1926 questioned its male origin, which raised doubts about its authenticity. Our report presents genetic evidence that resolves this dispute. From the sole tooth we obtained a sufficient amount of DNA to perform the analysis of nuclear markers. The analysis of the sex-informative part of intron 1 in amelogenin, genotyped using AmpFiSTR® NGM PCR Amplification Kit and Powerplex® ESI17 Kit human identification systems, revealed the female origin of the tooth. The female origin was further confirmed by the analysis of a portion of amelogenin intron 2, a microsatellite marker located on the X chromosome, as well as by a lack of signal from Y chromosomal microsatellite markers and the sex-determining region Y marker. Data obtained for two hypervariable regions, HVI and HVII, in mitochondrial DNA showed that mtDNA haplotype was relatively frequent among contemporary Europeans. The analysis of a set of single nucleotide polymorphisms relevant for prediction of the iris color indicated an 87% probability that the woman had hazel or brown eye color.
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spelling pubmed-31187272011-06-24 Genetic examination of the putative skull of Jan Kochanowski reveals its female sex Kupiec, Tomasz Branicki, Wojciech Croat Med J Case Report We report the results of genetic examination of the putative skull of Jan Kochanowski (1530-1584), a great Polish renaissance poet. The skull was retrieved in 1791 by historian Tadeusz Czacki from the Kochanowski family tomb and became the property of the Czartoryskis Museum in Krakow. An anthropological study in 1926 questioned its male origin, which raised doubts about its authenticity. Our report presents genetic evidence that resolves this dispute. From the sole tooth we obtained a sufficient amount of DNA to perform the analysis of nuclear markers. The analysis of the sex-informative part of intron 1 in amelogenin, genotyped using AmpFiSTR® NGM PCR Amplification Kit and Powerplex® ESI17 Kit human identification systems, revealed the female origin of the tooth. The female origin was further confirmed by the analysis of a portion of amelogenin intron 2, a microsatellite marker located on the X chromosome, as well as by a lack of signal from Y chromosomal microsatellite markers and the sex-determining region Y marker. Data obtained for two hypervariable regions, HVI and HVII, in mitochondrial DNA showed that mtDNA haplotype was relatively frequent among contemporary Europeans. The analysis of a set of single nucleotide polymorphisms relevant for prediction of the iris color indicated an 87% probability that the woman had hazel or brown eye color. Croatian Medical Schools 2011-06 /pmc/articles/PMC3118727/ /pubmed/21674838 http://dx.doi.org/10.3325/cmj.2011.52.403 Text en Copyright © 2011 by the Croatian Medical Journal. All rights reserved. http://creativecommons.org/licenses/by/2.5/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Case Report
Kupiec, Tomasz
Branicki, Wojciech
Genetic examination of the putative skull of Jan Kochanowski reveals its female sex
title Genetic examination of the putative skull of Jan Kochanowski reveals its female sex
title_full Genetic examination of the putative skull of Jan Kochanowski reveals its female sex
title_fullStr Genetic examination of the putative skull of Jan Kochanowski reveals its female sex
title_full_unstemmed Genetic examination of the putative skull of Jan Kochanowski reveals its female sex
title_short Genetic examination of the putative skull of Jan Kochanowski reveals its female sex
title_sort genetic examination of the putative skull of jan kochanowski reveals its female sex
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3118727/
https://www.ncbi.nlm.nih.gov/pubmed/21674838
http://dx.doi.org/10.3325/cmj.2011.52.403
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