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A high-throughput immobilized bead screen for stable proteins and multi-protein complexes

We describe an in vitro colony screen to identify Escherichia coli expressing soluble proteins and stable, assembled multiprotein complexes. Proteins with an N-terminal 6His tag and C-terminal green fluorescent protein (GFP) S11 tag are fluorescently labeled in cells by complementation with a coexpr...

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Detalles Bibliográficos
Autores principales:	Lockard, Meghan A., Listwan, Pawel, Pedelacq, Jean-Denis, Cabantous, Stéphanie, Nguyen, Hau B., Terwilliger, Thomas C., Waldo, Geoffrey S.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2011
Materias:	Original Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3118733/ https://www.ncbi.nlm.nih.gov/pubmed/21642284 http://dx.doi.org/10.1093/protein/gzr021

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