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Col V siRNA Engineered Tenocytes for Tendon Tissue Engineering
The presence of uniformly small collagen fibrils in tendon repair is believed to play a major role in suboptimal tendon healing. Collagen V is significantly elevated in healing tendons and plays an important role in fibrillogenesis. The objective of this study was to investigate the effect of a part...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3119690/ https://www.ncbi.nlm.nih.gov/pubmed/21713001 http://dx.doi.org/10.1371/journal.pone.0021154 |
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author | Lu, Ping Zhang, Guo Rong Song, Xing Hui Zou, Xiao Hui Wang, Lin Lin Ouyang, Hong Wei |
author_facet | Lu, Ping Zhang, Guo Rong Song, Xing Hui Zou, Xiao Hui Wang, Lin Lin Ouyang, Hong Wei |
author_sort | Lu, Ping |
collection | PubMed |
description | The presence of uniformly small collagen fibrils in tendon repair is believed to play a major role in suboptimal tendon healing. Collagen V is significantly elevated in healing tendons and plays an important role in fibrillogenesis. The objective of this study was to investigate the effect of a particular chain of collagen V on the fibrillogenesis of Sprague-Dawley rat tenocytes, as well as the efficacy of Col V siRNA engineered tenocytes for tendon tissue engineering. RNA interference gene therapy and a scaffold free tissue engineered tendon model were employed. The results showed that scaffold free tissue engineered tendon had tissue-specific tendon structure. Down regulation of collagen V α1 or α2 chains by siRNAs (Col5α1 siRNA, Col5α2 siRNA) had different effects on collagen I and decorin gene expressions. Col5α1 siRNA treated tenocytes had smaller collagen fibrils with abnormal morphology; while those Col5α2 siRNA treated tenocytes had the same morphology as normal tenocytes. Furthermore, it was found that tendons formed by coculture of Col5α1 siRNA treated tenocytes with normal tenocytes at a proper ratio had larger collagen fibrils and relative normal contour. Conclusively, it was demonstrated that Col V siRNA engineered tenocytes improved tendon tissue regeneration. And an optimal level of collagen V is vital in regulating collagen fibrillogenesis. This may provide a basis for future development of novel cellular- and molecular biology-based therapeutics for tendon diseases. |
format | Online Article Text |
id | pubmed-3119690 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-31196902011-06-27 Col V siRNA Engineered Tenocytes for Tendon Tissue Engineering Lu, Ping Zhang, Guo Rong Song, Xing Hui Zou, Xiao Hui Wang, Lin Lin Ouyang, Hong Wei PLoS One Research Article The presence of uniformly small collagen fibrils in tendon repair is believed to play a major role in suboptimal tendon healing. Collagen V is significantly elevated in healing tendons and plays an important role in fibrillogenesis. The objective of this study was to investigate the effect of a particular chain of collagen V on the fibrillogenesis of Sprague-Dawley rat tenocytes, as well as the efficacy of Col V siRNA engineered tenocytes for tendon tissue engineering. RNA interference gene therapy and a scaffold free tissue engineered tendon model were employed. The results showed that scaffold free tissue engineered tendon had tissue-specific tendon structure. Down regulation of collagen V α1 or α2 chains by siRNAs (Col5α1 siRNA, Col5α2 siRNA) had different effects on collagen I and decorin gene expressions. Col5α1 siRNA treated tenocytes had smaller collagen fibrils with abnormal morphology; while those Col5α2 siRNA treated tenocytes had the same morphology as normal tenocytes. Furthermore, it was found that tendons formed by coculture of Col5α1 siRNA treated tenocytes with normal tenocytes at a proper ratio had larger collagen fibrils and relative normal contour. Conclusively, it was demonstrated that Col V siRNA engineered tenocytes improved tendon tissue regeneration. And an optimal level of collagen V is vital in regulating collagen fibrillogenesis. This may provide a basis for future development of novel cellular- and molecular biology-based therapeutics for tendon diseases. Public Library of Science 2011-06-21 /pmc/articles/PMC3119690/ /pubmed/21713001 http://dx.doi.org/10.1371/journal.pone.0021154 Text en Lu et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Lu, Ping Zhang, Guo Rong Song, Xing Hui Zou, Xiao Hui Wang, Lin Lin Ouyang, Hong Wei Col V siRNA Engineered Tenocytes for Tendon Tissue Engineering |
title | Col V siRNA Engineered Tenocytes for Tendon Tissue Engineering |
title_full | Col V siRNA Engineered Tenocytes for Tendon Tissue Engineering |
title_fullStr | Col V siRNA Engineered Tenocytes for Tendon Tissue Engineering |
title_full_unstemmed | Col V siRNA Engineered Tenocytes for Tendon Tissue Engineering |
title_short | Col V siRNA Engineered Tenocytes for Tendon Tissue Engineering |
title_sort | col v sirna engineered tenocytes for tendon tissue engineering |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3119690/ https://www.ncbi.nlm.nih.gov/pubmed/21713001 http://dx.doi.org/10.1371/journal.pone.0021154 |
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