Down-regulation of miR-27a might inhibit proliferation and drug resistance of gastric cancer cells

AIMS: Here we aimed to firstly investigate the role of miR-27a in proliferation and multidrug resistance of gastric cancer cells. METHODS: The role of miR-27a in gastric cancer cells was detected using MTT assay, soft agar assay, flow cytometry assay, nude mice assay, real-time PCR, western blot and...

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Detalles Bibliográficos
Autores principales: Zhao, Xiaohong, Yang, Li, Hu, Jianguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3120716/
https://www.ncbi.nlm.nih.gov/pubmed/21569481
http://dx.doi.org/10.1186/1756-9966-30-55
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author Zhao, Xiaohong
Yang, Li
Hu, Jianguo
author_facet Zhao, Xiaohong
Yang, Li
Hu, Jianguo
author_sort Zhao, Xiaohong
collection PubMed
description AIMS: Here we aimed to firstly investigate the role of miR-27a in proliferation and multidrug resistance of gastric cancer cells. METHODS: The role of miR-27a in gastric cancer cells was detected using MTT assay, soft agar assay, flow cytometry assay, nude mice assay, real-time PCR, western blot and reporter gene assay, etc. RESULTS: Down-regulation of miR-27a could inhibit porliferation of gastric cancer cells in vitro and in vivo. Down-regulation of miR-27a could also confer sensitivity of drugs on gastric cancer cells, and might increase accumulation and decrease releasing amount of adriamycin in gastric cancer cells. Down-regulation of miR-27a could significantly decrease the expression of P-glycoprotein and the transcriptional activity of cyclin D1, and up-regulate the expression of p21. CONCLUSIONS: MiR-27a might play important roles in porliferation and drug resistance of gastric cancer. MiR-27a might be considered as a useful target for cancer therapy.
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spelling pubmed-31207162011-06-23 Down-regulation of miR-27a might inhibit proliferation and drug resistance of gastric cancer cells Zhao, Xiaohong Yang, Li Hu, Jianguo J Exp Clin Cancer Res Research AIMS: Here we aimed to firstly investigate the role of miR-27a in proliferation and multidrug resistance of gastric cancer cells. METHODS: The role of miR-27a in gastric cancer cells was detected using MTT assay, soft agar assay, flow cytometry assay, nude mice assay, real-time PCR, western blot and reporter gene assay, etc. RESULTS: Down-regulation of miR-27a could inhibit porliferation of gastric cancer cells in vitro and in vivo. Down-regulation of miR-27a could also confer sensitivity of drugs on gastric cancer cells, and might increase accumulation and decrease releasing amount of adriamycin in gastric cancer cells. Down-regulation of miR-27a could significantly decrease the expression of P-glycoprotein and the transcriptional activity of cyclin D1, and up-regulate the expression of p21. CONCLUSIONS: MiR-27a might play important roles in porliferation and drug resistance of gastric cancer. MiR-27a might be considered as a useful target for cancer therapy. BioMed Central 2011-05-13 /pmc/articles/PMC3120716/ /pubmed/21569481 http://dx.doi.org/10.1186/1756-9966-30-55 Text en Copyright ©2011 Zhao et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Zhao, Xiaohong
Yang, Li
Hu, Jianguo
Down-regulation of miR-27a might inhibit proliferation and drug resistance of gastric cancer cells
title Down-regulation of miR-27a might inhibit proliferation and drug resistance of gastric cancer cells
title_full Down-regulation of miR-27a might inhibit proliferation and drug resistance of gastric cancer cells
title_fullStr Down-regulation of miR-27a might inhibit proliferation and drug resistance of gastric cancer cells
title_full_unstemmed Down-regulation of miR-27a might inhibit proliferation and drug resistance of gastric cancer cells
title_short Down-regulation of miR-27a might inhibit proliferation and drug resistance of gastric cancer cells
title_sort down-regulation of mir-27a might inhibit proliferation and drug resistance of gastric cancer cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3120716/
https://www.ncbi.nlm.nih.gov/pubmed/21569481
http://dx.doi.org/10.1186/1756-9966-30-55
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AT hujianguo downregulationofmir27amightinhibitproliferationanddrugresistanceofgastriccancercells

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