Cargando…
Quantitation of Cellular Dynamics in Growing Arabidopsis Roots with Light Sheet Microscopy
To understand dynamic developmental processes, living tissues have to be imaged frequently and for extended periods of time. Root development is extensively studied at cellular resolution to understand basic mechanisms underlying pattern formation and maintenance in plants. Unfortunately, ensuring c...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3120859/ https://www.ncbi.nlm.nih.gov/pubmed/21731697 http://dx.doi.org/10.1371/journal.pone.0021303 |
_version_ | 1782206771910148096 |
---|---|
author | Sena, Giovanni Frentz, Zak Birnbaum, Kenneth D. Leibler, Stanislas |
author_facet | Sena, Giovanni Frentz, Zak Birnbaum, Kenneth D. Leibler, Stanislas |
author_sort | Sena, Giovanni |
collection | PubMed |
description | To understand dynamic developmental processes, living tissues have to be imaged frequently and for extended periods of time. Root development is extensively studied at cellular resolution to understand basic mechanisms underlying pattern formation and maintenance in plants. Unfortunately, ensuring continuous specimen access, while preserving physiological conditions and preventing photo-damage, poses major barriers to measurements of cellular dynamics in growing organs such as plant roots. We present a system that integrates optical sectioning through light sheet fluorescence microscopy with hydroponic culture that enables us to image, at cellular resolution, a vertically growing Arabidopsis root every few minutes and for several consecutive days. We describe novel automated routines to track the root tip as it grows, to track cellular nuclei and to identify cell divisions. We demonstrate the system's capabilities by collecting data on divisions and nuclear dynamics. |
format | Online Article Text |
id | pubmed-3120859 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-31208592011-06-30 Quantitation of Cellular Dynamics in Growing Arabidopsis Roots with Light Sheet Microscopy Sena, Giovanni Frentz, Zak Birnbaum, Kenneth D. Leibler, Stanislas PLoS One Research Article To understand dynamic developmental processes, living tissues have to be imaged frequently and for extended periods of time. Root development is extensively studied at cellular resolution to understand basic mechanisms underlying pattern formation and maintenance in plants. Unfortunately, ensuring continuous specimen access, while preserving physiological conditions and preventing photo-damage, poses major barriers to measurements of cellular dynamics in growing organs such as plant roots. We present a system that integrates optical sectioning through light sheet fluorescence microscopy with hydroponic culture that enables us to image, at cellular resolution, a vertically growing Arabidopsis root every few minutes and for several consecutive days. We describe novel automated routines to track the root tip as it grows, to track cellular nuclei and to identify cell divisions. We demonstrate the system's capabilities by collecting data on divisions and nuclear dynamics. Public Library of Science 2011-06-22 /pmc/articles/PMC3120859/ /pubmed/21731697 http://dx.doi.org/10.1371/journal.pone.0021303 Text en Sena et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Sena, Giovanni Frentz, Zak Birnbaum, Kenneth D. Leibler, Stanislas Quantitation of Cellular Dynamics in Growing Arabidopsis Roots with Light Sheet Microscopy |
title | Quantitation of Cellular Dynamics in Growing Arabidopsis Roots with Light Sheet Microscopy |
title_full | Quantitation of Cellular Dynamics in Growing Arabidopsis Roots with Light Sheet Microscopy |
title_fullStr | Quantitation of Cellular Dynamics in Growing Arabidopsis Roots with Light Sheet Microscopy |
title_full_unstemmed | Quantitation of Cellular Dynamics in Growing Arabidopsis Roots with Light Sheet Microscopy |
title_short | Quantitation of Cellular Dynamics in Growing Arabidopsis Roots with Light Sheet Microscopy |
title_sort | quantitation of cellular dynamics in growing arabidopsis roots with light sheet microscopy |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3120859/ https://www.ncbi.nlm.nih.gov/pubmed/21731697 http://dx.doi.org/10.1371/journal.pone.0021303 |
work_keys_str_mv | AT senagiovanni quantitationofcellulardynamicsingrowingarabidopsisrootswithlightsheetmicroscopy AT frentzzak quantitationofcellulardynamicsingrowingarabidopsisrootswithlightsheetmicroscopy AT birnbaumkennethd quantitationofcellulardynamicsingrowingarabidopsisrootswithlightsheetmicroscopy AT leiblerstanislas quantitationofcellulardynamicsingrowingarabidopsisrootswithlightsheetmicroscopy |