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Virulence attributes of Helicobacter pylori isolates & their association with gastroduodenal disease

BACKGROUND & OBJECTIVES: Certain genotype(s) of Helicobacter pylori strains may play important role in the development of gastric cancer (GC) and peptic ulcer disease (PUD). This study was undertaken to investigate the association of cagA, cagA3(/) region subtypes, babA2 and vacA genotypes of H....

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Autores principales: Saxena, A., Shukla, S., Prasad, K.N., Ghoshal, U.C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3121283/
https://www.ncbi.nlm.nih.gov/pubmed/21623037
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author Saxena, A.
Shukla, S.
Prasad, K.N.
Ghoshal, U.C.
author_facet Saxena, A.
Shukla, S.
Prasad, K.N.
Ghoshal, U.C.
author_sort Saxena, A.
collection PubMed
description BACKGROUND & OBJECTIVES: Certain genotype(s) of Helicobacter pylori strains may play important role in the development of gastric cancer (GC) and peptic ulcer disease (PUD). This study was undertaken to investigate the association of cagA, cagA3(/) region subtypes, babA2 and vacA genotypes of H. pylori with GC, PUD and non-ulcer dyspepsia (NUD) as there are no such studies from India. METHODS: A total of 348 consecutive adult patients (NUD 241, PUD 45, GC 62) undergoing upper gastrointestinal endoscopy between September 2002 and May 2007 in a tertiary referral centre at Lucknow, north India, were enrolled. H. pylori infection was diagnosed by rapid urease test, culture, histopathology and PCR. Genotyping for cagA, cagA3(/) subtypes, babA2 and vacA was performed by PCR using sequence specific primers. RESULTS: H. pylori infection was higher in patients with PUD than with GC (80 vs. 56.5%, P < 0.01) and NUD (80 vs. 55.2%, P= 0.002). cagA positive H. pylori isolates were detected in 80 per cent in GC, 83.3 per cent in PUD and 76.7 per cent in NUD with no significant difference among them. Only A subtype of cagA3(/) was detected and its distribution in GC, PUD and NUD was 68.8, 69.4 and 52.6 per cent respectively. Presence of babA2 genotype was 31.4 per cent and it had significant association with PUD when compared with NUD (52.8 vs. 26.3%, P<0.003). On univariate regression analysis, s1a allele was associated with GC (P<0.050) and s1a/m2 vacA genotype with both GC (P=0.014) and PUD (P=0.016). INTERPRETATION & CONCLUSIONS: H. pylori infection was strongly associated with PUD with a very high proportion of patients with GC have s1a allele and s1a/m2 vacA genotype. Both s1a/m2 vacA genotype and babA2 are associated with PUD. The study shows that different virulence attributes of H. pylori are involved in different gastroduodenal disorders.
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spelling pubmed-31212832011-07-01 Virulence attributes of Helicobacter pylori isolates & their association with gastroduodenal disease Saxena, A. Shukla, S. Prasad, K.N. Ghoshal, U.C. Indian J Med Res Original Article BACKGROUND & OBJECTIVES: Certain genotype(s) of Helicobacter pylori strains may play important role in the development of gastric cancer (GC) and peptic ulcer disease (PUD). This study was undertaken to investigate the association of cagA, cagA3(/) region subtypes, babA2 and vacA genotypes of H. pylori with GC, PUD and non-ulcer dyspepsia (NUD) as there are no such studies from India. METHODS: A total of 348 consecutive adult patients (NUD 241, PUD 45, GC 62) undergoing upper gastrointestinal endoscopy between September 2002 and May 2007 in a tertiary referral centre at Lucknow, north India, were enrolled. H. pylori infection was diagnosed by rapid urease test, culture, histopathology and PCR. Genotyping for cagA, cagA3(/) subtypes, babA2 and vacA was performed by PCR using sequence specific primers. RESULTS: H. pylori infection was higher in patients with PUD than with GC (80 vs. 56.5%, P < 0.01) and NUD (80 vs. 55.2%, P= 0.002). cagA positive H. pylori isolates were detected in 80 per cent in GC, 83.3 per cent in PUD and 76.7 per cent in NUD with no significant difference among them. Only A subtype of cagA3(/) was detected and its distribution in GC, PUD and NUD was 68.8, 69.4 and 52.6 per cent respectively. Presence of babA2 genotype was 31.4 per cent and it had significant association with PUD when compared with NUD (52.8 vs. 26.3%, P<0.003). On univariate regression analysis, s1a allele was associated with GC (P<0.050) and s1a/m2 vacA genotype with both GC (P=0.014) and PUD (P=0.016). INTERPRETATION & CONCLUSIONS: H. pylori infection was strongly associated with PUD with a very high proportion of patients with GC have s1a allele and s1a/m2 vacA genotype. Both s1a/m2 vacA genotype and babA2 are associated with PUD. The study shows that different virulence attributes of H. pylori are involved in different gastroduodenal disorders. Medknow Publications 2011-05 /pmc/articles/PMC3121283/ /pubmed/21623037 Text en © The Indian Journal of Medical Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Saxena, A.
Shukla, S.
Prasad, K.N.
Ghoshal, U.C.
Virulence attributes of Helicobacter pylori isolates & their association with gastroduodenal disease
title Virulence attributes of Helicobacter pylori isolates & their association with gastroduodenal disease
title_full Virulence attributes of Helicobacter pylori isolates & their association with gastroduodenal disease
title_fullStr Virulence attributes of Helicobacter pylori isolates & their association with gastroduodenal disease
title_full_unstemmed Virulence attributes of Helicobacter pylori isolates & their association with gastroduodenal disease
title_short Virulence attributes of Helicobacter pylori isolates & their association with gastroduodenal disease
title_sort virulence attributes of helicobacter pylori isolates & their association with gastroduodenal disease
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3121283/
https://www.ncbi.nlm.nih.gov/pubmed/21623037
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