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Functional Expression of Spider Neurotoxic Peptide Huwentoxin-I in E. coli
The coding sequence of huwentoxin-I, a neurotoxic peptide isolated from the venom of the Chinese spider Ornithoctonus huwena, was amplified by PCR using the cDNA library constructed from the spider venom glands. The cloned fragment was inserted into the expression vector pET-40b and transformed into...
Autores principales: | Meng, Er, Cai, Tian-Fu, Li, Wen-Ying, Zhang, Hui, Liu, Yan-Bo, Peng, Kuan, Liang, Songping, Zhang, Dong-Yi |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3121796/ https://www.ncbi.nlm.nih.gov/pubmed/21731778 http://dx.doi.org/10.1371/journal.pone.0021608 |
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