Rapid Detection of the H275Y Oseltamivir Resistance Mutation in Influenza A/H1N1 2009 by Single Base Pair RT-PCR and High-Resolution Melting

INTRODUCTION: We aimed to design a real-time reverse-transcriptase-PCR (rRT-PCR), high-resolution melting (HRM) assay to detect the H275Y mutation that confers oseltamivir resistance in influenza A/H1N1 2009 viruses. FINDINGS: A novel strategy of amplifying a single base pair, the relevant SNP at po...

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Detalles Bibliográficos
Autores principales: Tong, Steven Y. C., Dakh, Farshid, Hurt, Aeron C., Deng, Yi-Mo, Freeman, Kevin, Fagan, Peter K., Barr, Ian G., Giffard, Philip M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3123348/
https://www.ncbi.nlm.nih.gov/pubmed/21731753
http://dx.doi.org/10.1371/journal.pone.0021446

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3123348/
https://www.ncbi.nlm.nih.gov/pubmed/21731753
http://dx.doi.org/10.1371/journal.pone.0021446

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