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Rapid Detection of the H275Y Oseltamivir Resistance Mutation in Influenza A/H1N1 2009 by Single Base Pair RT-PCR and High-Resolution Melting
INTRODUCTION: We aimed to design a real-time reverse-transcriptase-PCR (rRT-PCR), high-resolution melting (HRM) assay to detect the H275Y mutation that confers oseltamivir resistance in influenza A/H1N1 2009 viruses. FINDINGS: A novel strategy of amplifying a single base pair, the relevant SNP at po...
Autores principales: | Tong, Steven Y. C., Dakh, Farshid, Hurt, Aeron C., Deng, Yi-Mo, Freeman, Kevin, Fagan, Peter K., Barr, Ian G., Giffard, Philip M. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3123348/ https://www.ncbi.nlm.nih.gov/pubmed/21731753 http://dx.doi.org/10.1371/journal.pone.0021446 |
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