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Schwann-Spheres Derived from Injured Peripheral Nerves in Adult Mice - Their In Vitro Characterization and Therapeutic Potential

Multipotent somatic stem cells have been identified in various adult tissues. However, the stem/progenitor cells of the peripheral nerves have been isolated only from fetal tissues. Here, we isolated Schwann-cell precursors/immature Schwann cells from the injured peripheral nerves of adult mice usin...

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Autores principales: Takagi, Takehiko, Ishii, Ken, Shibata, Shinsuke, Yasuda, Akimasa, Sato, Momoka, Nagoshi, Narihito, Saito, Harukazu, Okano, Hirotaka J., Toyama, Yoshiaki, Okano, Hideyuki, Nakamura, Masaya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3123355/
https://www.ncbi.nlm.nih.gov/pubmed/21720551
http://dx.doi.org/10.1371/journal.pone.0021497
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author Takagi, Takehiko
Ishii, Ken
Shibata, Shinsuke
Yasuda, Akimasa
Sato, Momoka
Nagoshi, Narihito
Saito, Harukazu
Okano, Hirotaka J.
Toyama, Yoshiaki
Okano, Hideyuki
Nakamura, Masaya
author_facet Takagi, Takehiko
Ishii, Ken
Shibata, Shinsuke
Yasuda, Akimasa
Sato, Momoka
Nagoshi, Narihito
Saito, Harukazu
Okano, Hirotaka J.
Toyama, Yoshiaki
Okano, Hideyuki
Nakamura, Masaya
author_sort Takagi, Takehiko
collection PubMed
description Multipotent somatic stem cells have been identified in various adult tissues. However, the stem/progenitor cells of the peripheral nerves have been isolated only from fetal tissues. Here, we isolated Schwann-cell precursors/immature Schwann cells from the injured peripheral nerves of adult mice using a floating culture technique that we call “Schwann-spheres." The Schwann-spheres were derived from de-differentiated mature Schwann cells harvested 24 hours to 6 weeks after peripheral nerve injury. They had extensive self-renewal and differentiation capabilities. They strongly expressed the immature-Schwann-cell marker p75, and differentiated only into the Schwann-cell lineage. The spheres showed enhanced myelin formation and neurite growth compared to mature Schwann cells in vitro. Mature Schwann cells have been considered a promising candidate for cell-transplantation therapies to repair the damaged nervous system, whereas these “Schwann-spheres" would provide a more potential autologous cell source for such transplantation.
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spelling pubmed-31233552011-06-29 Schwann-Spheres Derived from Injured Peripheral Nerves in Adult Mice - Their In Vitro Characterization and Therapeutic Potential Takagi, Takehiko Ishii, Ken Shibata, Shinsuke Yasuda, Akimasa Sato, Momoka Nagoshi, Narihito Saito, Harukazu Okano, Hirotaka J. Toyama, Yoshiaki Okano, Hideyuki Nakamura, Masaya PLoS One Research Article Multipotent somatic stem cells have been identified in various adult tissues. However, the stem/progenitor cells of the peripheral nerves have been isolated only from fetal tissues. Here, we isolated Schwann-cell precursors/immature Schwann cells from the injured peripheral nerves of adult mice using a floating culture technique that we call “Schwann-spheres." The Schwann-spheres were derived from de-differentiated mature Schwann cells harvested 24 hours to 6 weeks after peripheral nerve injury. They had extensive self-renewal and differentiation capabilities. They strongly expressed the immature-Schwann-cell marker p75, and differentiated only into the Schwann-cell lineage. The spheres showed enhanced myelin formation and neurite growth compared to mature Schwann cells in vitro. Mature Schwann cells have been considered a promising candidate for cell-transplantation therapies to repair the damaged nervous system, whereas these “Schwann-spheres" would provide a more potential autologous cell source for such transplantation. Public Library of Science 2011-06-24 /pmc/articles/PMC3123355/ /pubmed/21720551 http://dx.doi.org/10.1371/journal.pone.0021497 Text en Takagi et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Takagi, Takehiko
Ishii, Ken
Shibata, Shinsuke
Yasuda, Akimasa
Sato, Momoka
Nagoshi, Narihito
Saito, Harukazu
Okano, Hirotaka J.
Toyama, Yoshiaki
Okano, Hideyuki
Nakamura, Masaya
Schwann-Spheres Derived from Injured Peripheral Nerves in Adult Mice - Their In Vitro Characterization and Therapeutic Potential
title Schwann-Spheres Derived from Injured Peripheral Nerves in Adult Mice - Their In Vitro Characterization and Therapeutic Potential
title_full Schwann-Spheres Derived from Injured Peripheral Nerves in Adult Mice - Their In Vitro Characterization and Therapeutic Potential
title_fullStr Schwann-Spheres Derived from Injured Peripheral Nerves in Adult Mice - Their In Vitro Characterization and Therapeutic Potential
title_full_unstemmed Schwann-Spheres Derived from Injured Peripheral Nerves in Adult Mice - Their In Vitro Characterization and Therapeutic Potential
title_short Schwann-Spheres Derived from Injured Peripheral Nerves in Adult Mice - Their In Vitro Characterization and Therapeutic Potential
title_sort schwann-spheres derived from injured peripheral nerves in adult mice - their in vitro characterization and therapeutic potential
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3123355/
https://www.ncbi.nlm.nih.gov/pubmed/21720551
http://dx.doi.org/10.1371/journal.pone.0021497
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