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Brachypodium distachyon: a new pathosystem to study Fusarium head blight and other Fusarium diseases of wheat

BACKGROUND: Fusarium species cause Fusarium head blight (FHB) and other important diseases of cereals. The causal agents produce trichothecene mycotoxins such as deoxynivalenol (DON). The dicotyledonous model species Arabidopsis thaliana has been used to study Fusarium-host interactions but it is no...

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Autores principales: Peraldi, Antoine, Beccari, Giovanni, Steed, Andrew, Nicholson, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3123626/
https://www.ncbi.nlm.nih.gov/pubmed/21639892
http://dx.doi.org/10.1186/1471-2229-11-100
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author Peraldi, Antoine
Beccari, Giovanni
Steed, Andrew
Nicholson, Paul
author_facet Peraldi, Antoine
Beccari, Giovanni
Steed, Andrew
Nicholson, Paul
author_sort Peraldi, Antoine
collection PubMed
description BACKGROUND: Fusarium species cause Fusarium head blight (FHB) and other important diseases of cereals. The causal agents produce trichothecene mycotoxins such as deoxynivalenol (DON). The dicotyledonous model species Arabidopsis thaliana has been used to study Fusarium-host interactions but it is not ideal for model-to-crop translation. Brachypodium distachyon (Bd) has been proposed as a new monocotyledonous model species for functional genomic studies in grass species. This study aims to assess the interaction between the most prevalent FHB-causing Fusarium species and Bd in order to develop and exploit Bd as a genetic model for FHB and other Fusarium diseases of wheat. RESULTS: The ability of Fusarium graminearum and Fusarium culmorum to infect a range of Bd tissues was examined in various bioassays which showed that both species can infect all Bd tissues examined, including intact foliar tissues. DON accumulated in infected spike tissues at levels similar to those of infected wheat spikes. Histological studies revealed details of infection, colonisation and host response and indicate that hair cells are important sites of infection. Susceptibility to Fusarium and DON was assessed in two Bd ecotypes and revealed variation in resistance between ecotypes. CONCLUSIONS: Bd exhibits characteristics of susceptibility highly similar to those of wheat, including susceptibility to spread of disease in the spikelets. Bd is the first reported plant species to allow successful infection on intact foliar tissues by FHB-causing Fusarium species. DON appears to function as a virulence factor in Bd as it does in wheat. Bd is proposed as a valuable model for undertaking studies of Fusarium head blight and other Fusarium diseases of wheat.
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spelling pubmed-31236262011-06-26 Brachypodium distachyon: a new pathosystem to study Fusarium head blight and other Fusarium diseases of wheat Peraldi, Antoine Beccari, Giovanni Steed, Andrew Nicholson, Paul BMC Plant Biol Research Article BACKGROUND: Fusarium species cause Fusarium head blight (FHB) and other important diseases of cereals. The causal agents produce trichothecene mycotoxins such as deoxynivalenol (DON). The dicotyledonous model species Arabidopsis thaliana has been used to study Fusarium-host interactions but it is not ideal for model-to-crop translation. Brachypodium distachyon (Bd) has been proposed as a new monocotyledonous model species for functional genomic studies in grass species. This study aims to assess the interaction between the most prevalent FHB-causing Fusarium species and Bd in order to develop and exploit Bd as a genetic model for FHB and other Fusarium diseases of wheat. RESULTS: The ability of Fusarium graminearum and Fusarium culmorum to infect a range of Bd tissues was examined in various bioassays which showed that both species can infect all Bd tissues examined, including intact foliar tissues. DON accumulated in infected spike tissues at levels similar to those of infected wheat spikes. Histological studies revealed details of infection, colonisation and host response and indicate that hair cells are important sites of infection. Susceptibility to Fusarium and DON was assessed in two Bd ecotypes and revealed variation in resistance between ecotypes. CONCLUSIONS: Bd exhibits characteristics of susceptibility highly similar to those of wheat, including susceptibility to spread of disease in the spikelets. Bd is the first reported plant species to allow successful infection on intact foliar tissues by FHB-causing Fusarium species. DON appears to function as a virulence factor in Bd as it does in wheat. Bd is proposed as a valuable model for undertaking studies of Fusarium head blight and other Fusarium diseases of wheat. BioMed Central 2011-06-03 /pmc/articles/PMC3123626/ /pubmed/21639892 http://dx.doi.org/10.1186/1471-2229-11-100 Text en Copyright ©2011 Peraldi et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Peraldi, Antoine
Beccari, Giovanni
Steed, Andrew
Nicholson, Paul
Brachypodium distachyon: a new pathosystem to study Fusarium head blight and other Fusarium diseases of wheat
title Brachypodium distachyon: a new pathosystem to study Fusarium head blight and other Fusarium diseases of wheat
title_full Brachypodium distachyon: a new pathosystem to study Fusarium head blight and other Fusarium diseases of wheat
title_fullStr Brachypodium distachyon: a new pathosystem to study Fusarium head blight and other Fusarium diseases of wheat
title_full_unstemmed Brachypodium distachyon: a new pathosystem to study Fusarium head blight and other Fusarium diseases of wheat
title_short Brachypodium distachyon: a new pathosystem to study Fusarium head blight and other Fusarium diseases of wheat
title_sort brachypodium distachyon: a new pathosystem to study fusarium head blight and other fusarium diseases of wheat
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3123626/
https://www.ncbi.nlm.nih.gov/pubmed/21639892
http://dx.doi.org/10.1186/1471-2229-11-100
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