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Immortalized Adult Rodent Schwann Cells as In Vitro Models to Study Diabetic Neuropathy

We have established spontaneously immortalized Schwann cell lines from normal adult mice and rats and murine disease models. One of the normal mouse cell lines, IMS32, possesses some biological properties of mature Schwann cells and high proliferative activities. The IMS32 cells under hyperglycemic...

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Detalles Bibliográficos
Autores principales: Sango, Kazunori, Yanagisawa, Hiroko, Takaku, Shizuka, Kawakami, Emiko, Watabe, Kazuhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3124069/
https://www.ncbi.nlm.nih.gov/pubmed/21747827
http://dx.doi.org/10.1155/2011/374943
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author Sango, Kazunori
Yanagisawa, Hiroko
Takaku, Shizuka
Kawakami, Emiko
Watabe, Kazuhiko
author_facet Sango, Kazunori
Yanagisawa, Hiroko
Takaku, Shizuka
Kawakami, Emiko
Watabe, Kazuhiko
author_sort Sango, Kazunori
collection PubMed
description We have established spontaneously immortalized Schwann cell lines from normal adult mice and rats and murine disease models. One of the normal mouse cell lines, IMS32, possesses some biological properties of mature Schwann cells and high proliferative activities. The IMS32 cells under hyperglycemic and/or hyperlipidemic conditions have been utilized to investigate the pathogenesis of diabetic neuropathy, especially the polyol pathway hyperactivity, glycation, increased oxidative stress, and reduced synthesis of neurotrophic factors. In addition to the mouse cell lines, our current study focuses on the characterization of a normal rat cell line, IFRS1, under normal and high glucose conditions. These Schwann cell lines can be valuable tools for exploring the detailed mechanisms leading to diabetic neuropathy and novel therapeutic approaches against that condition.
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spelling pubmed-31240692011-07-11 Immortalized Adult Rodent Schwann Cells as In Vitro Models to Study Diabetic Neuropathy Sango, Kazunori Yanagisawa, Hiroko Takaku, Shizuka Kawakami, Emiko Watabe, Kazuhiko Exp Diabetes Res Review Article We have established spontaneously immortalized Schwann cell lines from normal adult mice and rats and murine disease models. One of the normal mouse cell lines, IMS32, possesses some biological properties of mature Schwann cells and high proliferative activities. The IMS32 cells under hyperglycemic and/or hyperlipidemic conditions have been utilized to investigate the pathogenesis of diabetic neuropathy, especially the polyol pathway hyperactivity, glycation, increased oxidative stress, and reduced synthesis of neurotrophic factors. In addition to the mouse cell lines, our current study focuses on the characterization of a normal rat cell line, IFRS1, under normal and high glucose conditions. These Schwann cell lines can be valuable tools for exploring the detailed mechanisms leading to diabetic neuropathy and novel therapeutic approaches against that condition. Hindawi Publishing Corporation 2011 2011-06-13 /pmc/articles/PMC3124069/ /pubmed/21747827 http://dx.doi.org/10.1155/2011/374943 Text en Copyright © 2011 Kazunori Sango et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Sango, Kazunori
Yanagisawa, Hiroko
Takaku, Shizuka
Kawakami, Emiko
Watabe, Kazuhiko
Immortalized Adult Rodent Schwann Cells as In Vitro Models to Study Diabetic Neuropathy
title Immortalized Adult Rodent Schwann Cells as In Vitro Models to Study Diabetic Neuropathy
title_full Immortalized Adult Rodent Schwann Cells as In Vitro Models to Study Diabetic Neuropathy
title_fullStr Immortalized Adult Rodent Schwann Cells as In Vitro Models to Study Diabetic Neuropathy
title_full_unstemmed Immortalized Adult Rodent Schwann Cells as In Vitro Models to Study Diabetic Neuropathy
title_short Immortalized Adult Rodent Schwann Cells as In Vitro Models to Study Diabetic Neuropathy
title_sort immortalized adult rodent schwann cells as in vitro models to study diabetic neuropathy
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3124069/
https://www.ncbi.nlm.nih.gov/pubmed/21747827
http://dx.doi.org/10.1155/2011/374943
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