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Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells
A gene delivery concept based on ethylenediamine-functionalized single-walled carbon nanotubes (f-SWCNTs) using the oncogene suppressor p53 gene as a model gene was successfully tested in vitro in MCF-7 breast cancer cells. The f-SWCNTs-p53 complexes were introduced into the cell medium at a concent...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3124390/ https://www.ncbi.nlm.nih.gov/pubmed/21720516 http://dx.doi.org/10.2147/IJN.S17684 |
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author | Karmakar, Alokita Bratton, Stacie M Dervishi, Enkeleda Ghosh, Anindya Mahmood, Meena Xu, Yang Saeed, Lamya Mohammed Mustafa, Thikra Casciano, Dan Radominska-Pandya, Anna Biris, Alexandru S |
author_facet | Karmakar, Alokita Bratton, Stacie M Dervishi, Enkeleda Ghosh, Anindya Mahmood, Meena Xu, Yang Saeed, Lamya Mohammed Mustafa, Thikra Casciano, Dan Radominska-Pandya, Anna Biris, Alexandru S |
author_sort | Karmakar, Alokita |
collection | PubMed |
description | A gene delivery concept based on ethylenediamine-functionalized single-walled carbon nanotubes (f-SWCNTs) using the oncogene suppressor p53 gene as a model gene was successfully tested in vitro in MCF-7 breast cancer cells. The f-SWCNTs-p53 complexes were introduced into the cell medium at a concentration of 20 μg mL(−1) and cells were exposed for 24, 48, and 72 hours. Standard ethidium bromide and acridine orange assays were used to detect apoptotic cells and indicated that a significantly larger percentage of the cells (approx 40%) were dead after 72 hours of exposure to f-SWCNTs-p53 as compared to the control cells, which were exposed to only p53 or f-SWCNTs, respectively. To further support the uptake and expression of the genes within the cells, green fluorescent protein-tagged p53, attached to the f-SWCNTs was added to the medium and the complex was observed to be strongly expressed in the cells. Moreover, caspase 3 activity was found to be highly enhanced in cells incubated with the f-SWCNTs-p53 complex, indicating strongly induced apoptosis. This system could be the foundation for novel gene delivery platforms based on the unique structural and morphological properties of multi-functional nanomaterials. |
format | Online Article Text |
id | pubmed-3124390 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-31243902011-06-29 Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells Karmakar, Alokita Bratton, Stacie M Dervishi, Enkeleda Ghosh, Anindya Mahmood, Meena Xu, Yang Saeed, Lamya Mohammed Mustafa, Thikra Casciano, Dan Radominska-Pandya, Anna Biris, Alexandru S Int J Nanomedicine Original Research A gene delivery concept based on ethylenediamine-functionalized single-walled carbon nanotubes (f-SWCNTs) using the oncogene suppressor p53 gene as a model gene was successfully tested in vitro in MCF-7 breast cancer cells. The f-SWCNTs-p53 complexes were introduced into the cell medium at a concentration of 20 μg mL(−1) and cells were exposed for 24, 48, and 72 hours. Standard ethidium bromide and acridine orange assays were used to detect apoptotic cells and indicated that a significantly larger percentage of the cells (approx 40%) were dead after 72 hours of exposure to f-SWCNTs-p53 as compared to the control cells, which were exposed to only p53 or f-SWCNTs, respectively. To further support the uptake and expression of the genes within the cells, green fluorescent protein-tagged p53, attached to the f-SWCNTs was added to the medium and the complex was observed to be strongly expressed in the cells. Moreover, caspase 3 activity was found to be highly enhanced in cells incubated with the f-SWCNTs-p53 complex, indicating strongly induced apoptosis. This system could be the foundation for novel gene delivery platforms based on the unique structural and morphological properties of multi-functional nanomaterials. Dove Medical Press 2011 2011-05-18 /pmc/articles/PMC3124390/ /pubmed/21720516 http://dx.doi.org/10.2147/IJN.S17684 Text en © 2011 Karmakar et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. |
spellingShingle | Original Research Karmakar, Alokita Bratton, Stacie M Dervishi, Enkeleda Ghosh, Anindya Mahmood, Meena Xu, Yang Saeed, Lamya Mohammed Mustafa, Thikra Casciano, Dan Radominska-Pandya, Anna Biris, Alexandru S Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells |
title | Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells |
title_full | Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells |
title_fullStr | Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells |
title_full_unstemmed | Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells |
title_short | Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells |
title_sort | ethylenediamine functionalized-single-walled nanotube (f-swnt)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer mcf-7 cells |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3124390/ https://www.ncbi.nlm.nih.gov/pubmed/21720516 http://dx.doi.org/10.2147/IJN.S17684 |
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