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Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells

A gene delivery concept based on ethylenediamine-functionalized single-walled carbon nanotubes (f-SWCNTs) using the oncogene suppressor p53 gene as a model gene was successfully tested in vitro in MCF-7 breast cancer cells. The f-SWCNTs-p53 complexes were introduced into the cell medium at a concent...

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Autores principales: Karmakar, Alokita, Bratton, Stacie M, Dervishi, Enkeleda, Ghosh, Anindya, Mahmood, Meena, Xu, Yang, Saeed, Lamya Mohammed, Mustafa, Thikra, Casciano, Dan, Radominska-Pandya, Anna, Biris, Alexandru S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3124390/
https://www.ncbi.nlm.nih.gov/pubmed/21720516
http://dx.doi.org/10.2147/IJN.S17684
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author Karmakar, Alokita
Bratton, Stacie M
Dervishi, Enkeleda
Ghosh, Anindya
Mahmood, Meena
Xu, Yang
Saeed, Lamya Mohammed
Mustafa, Thikra
Casciano, Dan
Radominska-Pandya, Anna
Biris, Alexandru S
author_facet Karmakar, Alokita
Bratton, Stacie M
Dervishi, Enkeleda
Ghosh, Anindya
Mahmood, Meena
Xu, Yang
Saeed, Lamya Mohammed
Mustafa, Thikra
Casciano, Dan
Radominska-Pandya, Anna
Biris, Alexandru S
author_sort Karmakar, Alokita
collection PubMed
description A gene delivery concept based on ethylenediamine-functionalized single-walled carbon nanotubes (f-SWCNTs) using the oncogene suppressor p53 gene as a model gene was successfully tested in vitro in MCF-7 breast cancer cells. The f-SWCNTs-p53 complexes were introduced into the cell medium at a concentration of 20 μg mL(−1) and cells were exposed for 24, 48, and 72 hours. Standard ethidium bromide and acridine orange assays were used to detect apoptotic cells and indicated that a significantly larger percentage of the cells (approx 40%) were dead after 72 hours of exposure to f-SWCNTs-p53 as compared to the control cells, which were exposed to only p53 or f-SWCNTs, respectively. To further support the uptake and expression of the genes within the cells, green fluorescent protein-tagged p53, attached to the f-SWCNTs was added to the medium and the complex was observed to be strongly expressed in the cells. Moreover, caspase 3 activity was found to be highly enhanced in cells incubated with the f-SWCNTs-p53 complex, indicating strongly induced apoptosis. This system could be the foundation for novel gene delivery platforms based on the unique structural and morphological properties of multi-functional nanomaterials.
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spelling pubmed-31243902011-06-29 Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells Karmakar, Alokita Bratton, Stacie M Dervishi, Enkeleda Ghosh, Anindya Mahmood, Meena Xu, Yang Saeed, Lamya Mohammed Mustafa, Thikra Casciano, Dan Radominska-Pandya, Anna Biris, Alexandru S Int J Nanomedicine Original Research A gene delivery concept based on ethylenediamine-functionalized single-walled carbon nanotubes (f-SWCNTs) using the oncogene suppressor p53 gene as a model gene was successfully tested in vitro in MCF-7 breast cancer cells. The f-SWCNTs-p53 complexes were introduced into the cell medium at a concentration of 20 μg mL(−1) and cells were exposed for 24, 48, and 72 hours. Standard ethidium bromide and acridine orange assays were used to detect apoptotic cells and indicated that a significantly larger percentage of the cells (approx 40%) were dead after 72 hours of exposure to f-SWCNTs-p53 as compared to the control cells, which were exposed to only p53 or f-SWCNTs, respectively. To further support the uptake and expression of the genes within the cells, green fluorescent protein-tagged p53, attached to the f-SWCNTs was added to the medium and the complex was observed to be strongly expressed in the cells. Moreover, caspase 3 activity was found to be highly enhanced in cells incubated with the f-SWCNTs-p53 complex, indicating strongly induced apoptosis. This system could be the foundation for novel gene delivery platforms based on the unique structural and morphological properties of multi-functional nanomaterials. Dove Medical Press 2011 2011-05-18 /pmc/articles/PMC3124390/ /pubmed/21720516 http://dx.doi.org/10.2147/IJN.S17684 Text en © 2011 Karmakar et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Karmakar, Alokita
Bratton, Stacie M
Dervishi, Enkeleda
Ghosh, Anindya
Mahmood, Meena
Xu, Yang
Saeed, Lamya Mohammed
Mustafa, Thikra
Casciano, Dan
Radominska-Pandya, Anna
Biris, Alexandru S
Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells
title Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells
title_full Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells
title_fullStr Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells
title_full_unstemmed Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells
title_short Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells
title_sort ethylenediamine functionalized-single-walled nanotube (f-swnt)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer mcf-7 cells
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3124390/
https://www.ncbi.nlm.nih.gov/pubmed/21720516
http://dx.doi.org/10.2147/IJN.S17684
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