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A study on the preparation and characterization of plasmid DNA and drug-containing magnetic nanoliposomes for the treatment of tumors

PURPOSE: To explore the preparation and characterization of a novel nanosized magnetic liposome containing the PEI-As(2)O(3)/Mn(0.5)Zn(0.5)Fe(2)O(4) complex. METHODS: Mn(0.5)Zn(0.5)Fe(2)O(4) and As(2)O(3)/Mn(0.5)Zn(0.5)Fe(2)O(4) nanoparticles were prepared by chemical coprecipitation and loaded with...

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Autores principales: Wang, Zi-Yu, Wang, Li, Zhang, Jia, Li, Yun-Tao, Zhang, Dong-Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3124393/
https://www.ncbi.nlm.nih.gov/pubmed/21720500
http://dx.doi.org/10.2147/IJN.S16485
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author Wang, Zi-Yu
Wang, Li
Zhang, Jia
Li, Yun-Tao
Zhang, Dong-Sheng
author_facet Wang, Zi-Yu
Wang, Li
Zhang, Jia
Li, Yun-Tao
Zhang, Dong-Sheng
author_sort Wang, Zi-Yu
collection PubMed
description PURPOSE: To explore the preparation and characterization of a novel nanosized magnetic liposome containing the PEI-As(2)O(3)/Mn(0.5)Zn(0.5)Fe(2)O(4) complex. METHODS: Mn(0.5)Zn(0.5)Fe(2)O(4) and As(2)O(3)/Mn(0.5)Zn(0.5)Fe(2)O(4) nanoparticles were prepared by chemical coprecipitation and loaded with PEI. The PEI- As(2)O(3)/Mn(0.5)Zn(0.5)Fe(2)O(4) complex was characterized using transmission electron and scanning electron microscopy, X-ray diffraction, energy dispersive spectrometry, and Fourier transform infrared spectroscopy. Cell transfection experiments were performed to evaluate the transfect efficiency. Magnetic nanoliposomes were prepared by rotatory evaporation and their shape, diameter, and thermodynamic characteristics were observed. RESULTS: Mn(0.5)Zn(0.5)Fe(2)O(4) and PEI-As(2)O(3)/Mn(0.5)Zn(0.5)Fe(2)O(4) nanoparticles were spherical, with an average diameter of 20–40 nm. PEI-As(2)O(3)/Mn(0.5)Zn(0.5)Fe(2)O(4) was an appropriate carrier for the delivery of a foreign gene to HepG2 cells. Energy dispersive spectrometry results confirmed the presence of the elements nitrogen and arsenic. Nanoliposomes of approximately 100 nm were observed under a transmission electron microscope. Upon exposure to an alternating magnetic field, they also had good magnetic responsiveness, even though Mn(0.5)Zn(0.5)Fe(2)O(4)was modified by PEI and encased in liposomes. Temperatures increased to 37°C–54°C depending on different concentrations of PEI-As(2)O(3)/Mn(0.5)Zn(0.5)Fe(2)O(4)and remained stable thereafter. CONCLUSION: Our results suggest that PEI-As(2)O(3)/Mn(0.5)Zn(0.5)Fe(2)O(4) magnetic nanoliposomes are an excellent biomaterial, which has multiple benefits in tumor thermotherapy, gene therapy, and chemotherapy.
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spelling pubmed-31243932011-06-29 A study on the preparation and characterization of plasmid DNA and drug-containing magnetic nanoliposomes for the treatment of tumors Wang, Zi-Yu Wang, Li Zhang, Jia Li, Yun-Tao Zhang, Dong-Sheng Int J Nanomedicine Original Research PURPOSE: To explore the preparation and characterization of a novel nanosized magnetic liposome containing the PEI-As(2)O(3)/Mn(0.5)Zn(0.5)Fe(2)O(4) complex. METHODS: Mn(0.5)Zn(0.5)Fe(2)O(4) and As(2)O(3)/Mn(0.5)Zn(0.5)Fe(2)O(4) nanoparticles were prepared by chemical coprecipitation and loaded with PEI. The PEI- As(2)O(3)/Mn(0.5)Zn(0.5)Fe(2)O(4) complex was characterized using transmission electron and scanning electron microscopy, X-ray diffraction, energy dispersive spectrometry, and Fourier transform infrared spectroscopy. Cell transfection experiments were performed to evaluate the transfect efficiency. Magnetic nanoliposomes were prepared by rotatory evaporation and their shape, diameter, and thermodynamic characteristics were observed. RESULTS: Mn(0.5)Zn(0.5)Fe(2)O(4) and PEI-As(2)O(3)/Mn(0.5)Zn(0.5)Fe(2)O(4) nanoparticles were spherical, with an average diameter of 20–40 nm. PEI-As(2)O(3)/Mn(0.5)Zn(0.5)Fe(2)O(4) was an appropriate carrier for the delivery of a foreign gene to HepG2 cells. Energy dispersive spectrometry results confirmed the presence of the elements nitrogen and arsenic. Nanoliposomes of approximately 100 nm were observed under a transmission electron microscope. Upon exposure to an alternating magnetic field, they also had good magnetic responsiveness, even though Mn(0.5)Zn(0.5)Fe(2)O(4)was modified by PEI and encased in liposomes. Temperatures increased to 37°C–54°C depending on different concentrations of PEI-As(2)O(3)/Mn(0.5)Zn(0.5)Fe(2)O(4)and remained stable thereafter. CONCLUSION: Our results suggest that PEI-As(2)O(3)/Mn(0.5)Zn(0.5)Fe(2)O(4) magnetic nanoliposomes are an excellent biomaterial, which has multiple benefits in tumor thermotherapy, gene therapy, and chemotherapy. Dove Medical Press 2011 2011-04-27 /pmc/articles/PMC3124393/ /pubmed/21720500 http://dx.doi.org/10.2147/IJN.S16485 Text en © 2011 Wang et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Wang, Zi-Yu
Wang, Li
Zhang, Jia
Li, Yun-Tao
Zhang, Dong-Sheng
A study on the preparation and characterization of plasmid DNA and drug-containing magnetic nanoliposomes for the treatment of tumors
title A study on the preparation and characterization of plasmid DNA and drug-containing magnetic nanoliposomes for the treatment of tumors
title_full A study on the preparation and characterization of plasmid DNA and drug-containing magnetic nanoliposomes for the treatment of tumors
title_fullStr A study on the preparation and characterization of plasmid DNA and drug-containing magnetic nanoliposomes for the treatment of tumors
title_full_unstemmed A study on the preparation and characterization of plasmid DNA and drug-containing magnetic nanoliposomes for the treatment of tumors
title_short A study on the preparation and characterization of plasmid DNA and drug-containing magnetic nanoliposomes for the treatment of tumors
title_sort study on the preparation and characterization of plasmid dna and drug-containing magnetic nanoliposomes for the treatment of tumors
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3124393/
https://www.ncbi.nlm.nih.gov/pubmed/21720500
http://dx.doi.org/10.2147/IJN.S16485
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