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Type and composition of surfactants mediating gene transfection of polyethylenimine-coated liposomes

BACKGROUND: The objective of this study was to compare the transfection efficiency of anionic liposomes coated with polyethylenimine (PEI) with that of PEI and Lipofectamine 2000(™) using the plasmid DNA encoding green fluorescent protein in a human hepatoma (Huh7) cell line. METHODS: Factors affect...

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Autores principales: Opanasopit, Praneet, Paecharoenchai, Orapan, Rojanarata, Theerasak, Ngawhirunpat, Tanasait, Ruktanonchai, Uracha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3124401/
https://www.ncbi.nlm.nih.gov/pubmed/21720509
http://dx.doi.org/10.2147/IJN.S18647
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author Opanasopit, Praneet
Paecharoenchai, Orapan
Rojanarata, Theerasak
Ngawhirunpat, Tanasait
Ruktanonchai, Uracha
author_facet Opanasopit, Praneet
Paecharoenchai, Orapan
Rojanarata, Theerasak
Ngawhirunpat, Tanasait
Ruktanonchai, Uracha
author_sort Opanasopit, Praneet
collection PubMed
description BACKGROUND: The objective of this study was to compare the transfection efficiency of anionic liposomes coated with polyethylenimine (PEI) with that of PEI and Lipofectamine 2000(™) using the plasmid DNA encoding green fluorescent protein in a human hepatoma (Huh7) cell line. METHODS: Factors affecting transfection efficiency, including type of surfactant, ratio of phosphatidylcholine (PC)/surfactant, carrier/DNA weight ratio, and the presence of serum have been investigated. Anionic liposomes, composed of PC and anionic surfactants, ie, sodium oleate (NaO), sodium taurocholate (NaT), or zwitterionic surfactant (3-[{3-cholamidopropyl}-dimethylammonio]-1-propanesulfonate, CHAPS) at molar ratios of 10:1, 10:1.5, and 10:2 were prepared by the sonication method. Subsequently, they were coated with PEI to produce polycationic liposomes (PCL). RESULTS: PCL was able to condense with pDNA depending on the PCL/DNA weight ratio. PCL composed of PC:NaO (10:2) showed higher transfection efficiency than NaT and CHAPS at all weight ratios tested. Higher transfection efficiency and gene expression were observed when the carrier/DNA weight ratio increased. The highest transfection efficiency was found at a weight ratio of 0.5. CONCLUSION: This PCL showed remarkably high transfection efficiency with low cytotoxicity to Huh7 cells in vitro, in comparison with PEI and Lipofectamine 2000.
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spelling pubmed-31244012011-06-29 Type and composition of surfactants mediating gene transfection of polyethylenimine-coated liposomes Opanasopit, Praneet Paecharoenchai, Orapan Rojanarata, Theerasak Ngawhirunpat, Tanasait Ruktanonchai, Uracha Int J Nanomedicine Original Research BACKGROUND: The objective of this study was to compare the transfection efficiency of anionic liposomes coated with polyethylenimine (PEI) with that of PEI and Lipofectamine 2000(™) using the plasmid DNA encoding green fluorescent protein in a human hepatoma (Huh7) cell line. METHODS: Factors affecting transfection efficiency, including type of surfactant, ratio of phosphatidylcholine (PC)/surfactant, carrier/DNA weight ratio, and the presence of serum have been investigated. Anionic liposomes, composed of PC and anionic surfactants, ie, sodium oleate (NaO), sodium taurocholate (NaT), or zwitterionic surfactant (3-[{3-cholamidopropyl}-dimethylammonio]-1-propanesulfonate, CHAPS) at molar ratios of 10:1, 10:1.5, and 10:2 were prepared by the sonication method. Subsequently, they were coated with PEI to produce polycationic liposomes (PCL). RESULTS: PCL was able to condense with pDNA depending on the PCL/DNA weight ratio. PCL composed of PC:NaO (10:2) showed higher transfection efficiency than NaT and CHAPS at all weight ratios tested. Higher transfection efficiency and gene expression were observed when the carrier/DNA weight ratio increased. The highest transfection efficiency was found at a weight ratio of 0.5. CONCLUSION: This PCL showed remarkably high transfection efficiency with low cytotoxicity to Huh7 cells in vitro, in comparison with PEI and Lipofectamine 2000. Dove Medical Press 2011 2011-05-10 /pmc/articles/PMC3124401/ /pubmed/21720509 http://dx.doi.org/10.2147/IJN.S18647 Text en © 2011 Opanasopit et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Opanasopit, Praneet
Paecharoenchai, Orapan
Rojanarata, Theerasak
Ngawhirunpat, Tanasait
Ruktanonchai, Uracha
Type and composition of surfactants mediating gene transfection of polyethylenimine-coated liposomes
title Type and composition of surfactants mediating gene transfection of polyethylenimine-coated liposomes
title_full Type and composition of surfactants mediating gene transfection of polyethylenimine-coated liposomes
title_fullStr Type and composition of surfactants mediating gene transfection of polyethylenimine-coated liposomes
title_full_unstemmed Type and composition of surfactants mediating gene transfection of polyethylenimine-coated liposomes
title_short Type and composition of surfactants mediating gene transfection of polyethylenimine-coated liposomes
title_sort type and composition of surfactants mediating gene transfection of polyethylenimine-coated liposomes
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3124401/
https://www.ncbi.nlm.nih.gov/pubmed/21720509
http://dx.doi.org/10.2147/IJN.S18647
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