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A Fast Optical Method for the Determination of Liquid Levels in Microplates
Parallel liquid handling systems are widely used in different applications of life sciences. In order to avoid false positive or negative results which lead to higher costs due to the replication of the experiments it is necessary to monitor the process and success of liquid delivery. An easy method...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3124871/ https://www.ncbi.nlm.nih.gov/pubmed/21747734 http://dx.doi.org/10.1155/2011/805153 |
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author | Thurow, Kerstin Stoll, Norbert Ritterbusch, Kai |
author_facet | Thurow, Kerstin Stoll, Norbert Ritterbusch, Kai |
author_sort | Thurow, Kerstin |
collection | PubMed |
description | Parallel liquid handling systems are widely used in different applications of life sciences. In order to avoid false positive or negative results which lead to higher costs due to the replication of the experiments it is necessary to monitor the process and success of liquid delivery. An easy method for the determination of the liquid levels in microplates has been developed and evaluated. The optical method bases on the measurement of the liquid level using CCD cameras followed by special algorithms for the evaluation and visualization of the measured data. The proposed method was tested in changing environmental lighting for two different liquids. These tests confirm our approach towards optical liquid level determination for smallest volumes in microplates and also show the challenges regarding environmental lighting and different physical properties of fluids. |
format | Online Article Text |
id | pubmed-3124871 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-31248712011-07-11 A Fast Optical Method for the Determination of Liquid Levels in Microplates Thurow, Kerstin Stoll, Norbert Ritterbusch, Kai J Autom Methods Manag Chem Research Article Parallel liquid handling systems are widely used in different applications of life sciences. In order to avoid false positive or negative results which lead to higher costs due to the replication of the experiments it is necessary to monitor the process and success of liquid delivery. An easy method for the determination of the liquid levels in microplates has been developed and evaluated. The optical method bases on the measurement of the liquid level using CCD cameras followed by special algorithms for the evaluation and visualization of the measured data. The proposed method was tested in changing environmental lighting for two different liquids. These tests confirm our approach towards optical liquid level determination for smallest volumes in microplates and also show the challenges regarding environmental lighting and different physical properties of fluids. Hindawi Publishing Corporation 2011 2011-06-18 /pmc/articles/PMC3124871/ /pubmed/21747734 http://dx.doi.org/10.1155/2011/805153 Text en Copyright © 2011 Kerstin Thurow et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Thurow, Kerstin Stoll, Norbert Ritterbusch, Kai A Fast Optical Method for the Determination of Liquid Levels in Microplates |
title | A Fast Optical Method for the Determination of Liquid Levels in Microplates |
title_full | A Fast Optical Method for the Determination of Liquid Levels in Microplates |
title_fullStr | A Fast Optical Method for the Determination of Liquid Levels in Microplates |
title_full_unstemmed | A Fast Optical Method for the Determination of Liquid Levels in Microplates |
title_short | A Fast Optical Method for the Determination of Liquid Levels in Microplates |
title_sort | fast optical method for the determination of liquid levels in microplates |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3124871/ https://www.ncbi.nlm.nih.gov/pubmed/21747734 http://dx.doi.org/10.1155/2011/805153 |
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