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Bone turnover in passive smoking female rat: relationships to change in bone mineral density
BACKGROUND: Many studies have identified smoking as a risk factor for osteoporosis, but it is unclear whether passive smoking has an effect on bone mineral density and bone turnover and if such an effect could cause osteoporosis.The purpose of the study was to investigate the effect of passive smoki...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3125254/ https://www.ncbi.nlm.nih.gov/pubmed/21663694 http://dx.doi.org/10.1186/1471-2474-12-131 |
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author | Gao, Shu-guang Li, Kang-hua Xu, Mai Jiang, Wei Shen, Hong Luo, Wei Xu, Wen-shuo Tian, Jian Lei, Guang-hua |
author_facet | Gao, Shu-guang Li, Kang-hua Xu, Mai Jiang, Wei Shen, Hong Luo, Wei Xu, Wen-shuo Tian, Jian Lei, Guang-hua |
author_sort | Gao, Shu-guang |
collection | PubMed |
description | BACKGROUND: Many studies have identified smoking as a risk factor for osteoporosis, but it is unclear whether passive smoking has an effect on bone mineral density and bone turnover and if such an effect could cause osteoporosis.The purpose of the study was to investigate the effect of passive smoking on bone mineral density (BMD) and bone turnover and the relationship between BMD and bone turnover in female rat. METHODS: Forty-eight female Wistar rats were randomized into six groups: 2-month, 3-month,4-month smoke-exposed rats and their controls. A rat model of passive cigarette smoking was prepared by breeding female rats in a cigarette-smoking box for 2, 3 or 4 months. Serums were analyzed for levels of osteocalcin, bone-specific alkaline phosphatase (b-ALP) and Tartrate-resistant acid phosphatase 5b (TRACP 5b). BMD was assessed at lumbar vertebrae and femur by dual energy X-ray absorptiometry in passive smoking rats and in control rats. RESULTS: BMD of lumbar spine and femur was lower in 4-month smoke-exposed female rats than that in controls. However, there was no significant difference in serum osteocalcin levels between smoke-exposed rats and controls. Significantly lower b-ALP and higher TRACP 5b were found in the 3-month or 4-month smoke-exposed rats compared to controls. Subsequent analysis showed that b-ALP positively correlated with BMD of the lumbar vertebrae(r = 0.764, P = 0.027) and femur(r = 0.899, P = 0.002) in 4-month smoke-exposed female rats. Furthermore, TRACP 5b levels negatively correlated with BMD of lumbar vertebrae (r = -0.871, P = 0.005) and femur (r = -0.715, P = 0.046) in 4-month smoke-exposed female rats. CONCLUSION: Our data suggest that smoke exposure can inhibit bone formation and increase bone resorption. The hazardous effects of passive smoking on bone status are associated with increased bone turnover in female rat. |
format | Online Article Text |
id | pubmed-3125254 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-31252542011-06-29 Bone turnover in passive smoking female rat: relationships to change in bone mineral density Gao, Shu-guang Li, Kang-hua Xu, Mai Jiang, Wei Shen, Hong Luo, Wei Xu, Wen-shuo Tian, Jian Lei, Guang-hua BMC Musculoskelet Disord Research Article BACKGROUND: Many studies have identified smoking as a risk factor for osteoporosis, but it is unclear whether passive smoking has an effect on bone mineral density and bone turnover and if such an effect could cause osteoporosis.The purpose of the study was to investigate the effect of passive smoking on bone mineral density (BMD) and bone turnover and the relationship between BMD and bone turnover in female rat. METHODS: Forty-eight female Wistar rats were randomized into six groups: 2-month, 3-month,4-month smoke-exposed rats and their controls. A rat model of passive cigarette smoking was prepared by breeding female rats in a cigarette-smoking box for 2, 3 or 4 months. Serums were analyzed for levels of osteocalcin, bone-specific alkaline phosphatase (b-ALP) and Tartrate-resistant acid phosphatase 5b (TRACP 5b). BMD was assessed at lumbar vertebrae and femur by dual energy X-ray absorptiometry in passive smoking rats and in control rats. RESULTS: BMD of lumbar spine and femur was lower in 4-month smoke-exposed female rats than that in controls. However, there was no significant difference in serum osteocalcin levels between smoke-exposed rats and controls. Significantly lower b-ALP and higher TRACP 5b were found in the 3-month or 4-month smoke-exposed rats compared to controls. Subsequent analysis showed that b-ALP positively correlated with BMD of the lumbar vertebrae(r = 0.764, P = 0.027) and femur(r = 0.899, P = 0.002) in 4-month smoke-exposed female rats. Furthermore, TRACP 5b levels negatively correlated with BMD of lumbar vertebrae (r = -0.871, P = 0.005) and femur (r = -0.715, P = 0.046) in 4-month smoke-exposed female rats. CONCLUSION: Our data suggest that smoke exposure can inhibit bone formation and increase bone resorption. The hazardous effects of passive smoking on bone status are associated with increased bone turnover in female rat. BioMed Central 2011-06-11 /pmc/articles/PMC3125254/ /pubmed/21663694 http://dx.doi.org/10.1186/1471-2474-12-131 Text en Copyright ©2011 Gao et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Gao, Shu-guang Li, Kang-hua Xu, Mai Jiang, Wei Shen, Hong Luo, Wei Xu, Wen-shuo Tian, Jian Lei, Guang-hua Bone turnover in passive smoking female rat: relationships to change in bone mineral density |
title | Bone turnover in passive smoking female rat: relationships to change in bone mineral density |
title_full | Bone turnover in passive smoking female rat: relationships to change in bone mineral density |
title_fullStr | Bone turnover in passive smoking female rat: relationships to change in bone mineral density |
title_full_unstemmed | Bone turnover in passive smoking female rat: relationships to change in bone mineral density |
title_short | Bone turnover in passive smoking female rat: relationships to change in bone mineral density |
title_sort | bone turnover in passive smoking female rat: relationships to change in bone mineral density |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3125254/ https://www.ncbi.nlm.nih.gov/pubmed/21663694 http://dx.doi.org/10.1186/1471-2474-12-131 |
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