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Temperature and time stability of whole blood lactate: implications for feasibility of pre-hospital measurement
BACKGROUND: To determine the time and temperature stability of whole blood lactate using experimental conditions applicable to the out-of-hospital environment. FINDINGS: We performed a prospective, clinical laboratory-based study at an academic hospital. Whole blood lactate was obtained by venipunct...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3125345/ https://www.ncbi.nlm.nih.gov/pubmed/21624139 http://dx.doi.org/10.1186/1756-0500-4-169 |
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author | Seymour, Christopher W Carlbom, David Cooke, Colin R Watkins, Timothy R Bulger, Eileen M Rea, Thomas D Baird, Geoffrey S |
author_facet | Seymour, Christopher W Carlbom, David Cooke, Colin R Watkins, Timothy R Bulger, Eileen M Rea, Thomas D Baird, Geoffrey S |
author_sort | Seymour, Christopher W |
collection | PubMed |
description | BACKGROUND: To determine the time and temperature stability of whole blood lactate using experimental conditions applicable to the out-of-hospital environment. FINDINGS: We performed a prospective, clinical laboratory-based study at an academic hospital. Whole blood lactate was obtained by venipuncture from five post-prandial, resting subjects. Blood was stored in lithium heparinized vacutainers in three temperature conditions: 1) room temperature (20°C), 2) wrapped in a portable, instant ice pack (0°C), or 3) wet ice (0°C). Lactate concentrations (mmol/L) were measured at 0, 5, 10, 20, and 30 minutes after sampling, and compared using repeated measures analysis of variance. Mean baseline lactate among resting subjects (N = 5) was 1.24 mmol/L (95%CI: 0.49,1.98 mmol/L). After 30 minutes, lactate concentration increased, on average, by 0.08 mmol/L (95%CI: 0.02,0.13 mmol/L), 0.18 mmol/L (95%CI: 0.07,0.28 mmol/L), and 0.36 mmol/L (95%CI: 0.24,0.47 mmol/L) when stored in wet ice, ice pack, and room temperature, respectively. The increase in lactate was similar in samples wrapped in portable ice pack or stored in wet ice at all time points (p > 0.05), and met criteria for equivalence at 30 minutes. However, lactate measurements from whole blood stored at room temperature were significantly greater, on average, than wet ice or portable ice pack within five and ten minutes, respectively (p < 0.05). CONCLUSIONS: Whole blood lactate measurements using samples stored in a portable ice pack are similar to wet ice for up to 30 minutes. These conditions are applicable to the out-of-hospital environment, and should inform future studies of pre-hospital measurement of lactate. |
format | Online Article Text |
id | pubmed-3125345 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-31253452011-06-29 Temperature and time stability of whole blood lactate: implications for feasibility of pre-hospital measurement Seymour, Christopher W Carlbom, David Cooke, Colin R Watkins, Timothy R Bulger, Eileen M Rea, Thomas D Baird, Geoffrey S BMC Res Notes Short Report BACKGROUND: To determine the time and temperature stability of whole blood lactate using experimental conditions applicable to the out-of-hospital environment. FINDINGS: We performed a prospective, clinical laboratory-based study at an academic hospital. Whole blood lactate was obtained by venipuncture from five post-prandial, resting subjects. Blood was stored in lithium heparinized vacutainers in three temperature conditions: 1) room temperature (20°C), 2) wrapped in a portable, instant ice pack (0°C), or 3) wet ice (0°C). Lactate concentrations (mmol/L) were measured at 0, 5, 10, 20, and 30 minutes after sampling, and compared using repeated measures analysis of variance. Mean baseline lactate among resting subjects (N = 5) was 1.24 mmol/L (95%CI: 0.49,1.98 mmol/L). After 30 minutes, lactate concentration increased, on average, by 0.08 mmol/L (95%CI: 0.02,0.13 mmol/L), 0.18 mmol/L (95%CI: 0.07,0.28 mmol/L), and 0.36 mmol/L (95%CI: 0.24,0.47 mmol/L) when stored in wet ice, ice pack, and room temperature, respectively. The increase in lactate was similar in samples wrapped in portable ice pack or stored in wet ice at all time points (p > 0.05), and met criteria for equivalence at 30 minutes. However, lactate measurements from whole blood stored at room temperature were significantly greater, on average, than wet ice or portable ice pack within five and ten minutes, respectively (p < 0.05). CONCLUSIONS: Whole blood lactate measurements using samples stored in a portable ice pack are similar to wet ice for up to 30 minutes. These conditions are applicable to the out-of-hospital environment, and should inform future studies of pre-hospital measurement of lactate. BioMed Central 2011-05-30 /pmc/articles/PMC3125345/ /pubmed/21624139 http://dx.doi.org/10.1186/1756-0500-4-169 Text en Copyright ©2011 Seymour et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Report Seymour, Christopher W Carlbom, David Cooke, Colin R Watkins, Timothy R Bulger, Eileen M Rea, Thomas D Baird, Geoffrey S Temperature and time stability of whole blood lactate: implications for feasibility of pre-hospital measurement |
title | Temperature and time stability of whole blood lactate: implications for feasibility of pre-hospital measurement |
title_full | Temperature and time stability of whole blood lactate: implications for feasibility of pre-hospital measurement |
title_fullStr | Temperature and time stability of whole blood lactate: implications for feasibility of pre-hospital measurement |
title_full_unstemmed | Temperature and time stability of whole blood lactate: implications for feasibility of pre-hospital measurement |
title_short | Temperature and time stability of whole blood lactate: implications for feasibility of pre-hospital measurement |
title_sort | temperature and time stability of whole blood lactate: implications for feasibility of pre-hospital measurement |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3125345/ https://www.ncbi.nlm.nih.gov/pubmed/21624139 http://dx.doi.org/10.1186/1756-0500-4-169 |
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