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An XMRV Derived Retroviral Vector as a Tool for Gene Transfer
BACKGROUND: Retroviral vectors are widely used tools for gene delivery and gene therapy. They are useful for gene expression studies and genetic manipulation in vitro and in vivo. Many retroviral vectors are derived from the mouse gammaretrovirus, murine leukemia virus (MLV). These vectors have been...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3125358/ https://www.ncbi.nlm.nih.gov/pubmed/21651801 http://dx.doi.org/10.1186/1743-422X-8-284 |
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author | Cervantes-Garcia, Daniel Rojas-Martinez, Augusto Camerini, David |
author_facet | Cervantes-Garcia, Daniel Rojas-Martinez, Augusto Camerini, David |
author_sort | Cervantes-Garcia, Daniel |
collection | PubMed |
description | BACKGROUND: Retroviral vectors are widely used tools for gene delivery and gene therapy. They are useful for gene expression studies and genetic manipulation in vitro and in vivo. Many retroviral vectors are derived from the mouse gammaretrovirus, murine leukemia virus (MLV). These vectors have been widely used in gene therapy clinical trials. XMRV, initially found in prostate cancer tissue, was the first human gammaretrovirus described. FINDINGS: We developed a new retroviral vector based on XMRV called pXC. It was developed for gene transfer to human cells and is produced by transient cotransfection of LNCaP cells with pXC and XMRV-packaging plasmids. CONCLUSIONS: We demonstrated that pXC mediates expression of inserted transgenes in cell lines. This new vector will be a useful tool for gene transfer in human and non-human cell lines, including gene therapy studies. |
format | Online Article Text |
id | pubmed-3125358 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-31253582011-06-29 An XMRV Derived Retroviral Vector as a Tool for Gene Transfer Cervantes-Garcia, Daniel Rojas-Martinez, Augusto Camerini, David Virol J Short Report BACKGROUND: Retroviral vectors are widely used tools for gene delivery and gene therapy. They are useful for gene expression studies and genetic manipulation in vitro and in vivo. Many retroviral vectors are derived from the mouse gammaretrovirus, murine leukemia virus (MLV). These vectors have been widely used in gene therapy clinical trials. XMRV, initially found in prostate cancer tissue, was the first human gammaretrovirus described. FINDINGS: We developed a new retroviral vector based on XMRV called pXC. It was developed for gene transfer to human cells and is produced by transient cotransfection of LNCaP cells with pXC and XMRV-packaging plasmids. CONCLUSIONS: We demonstrated that pXC mediates expression of inserted transgenes in cell lines. This new vector will be a useful tool for gene transfer in human and non-human cell lines, including gene therapy studies. BioMed Central 2011-06-08 /pmc/articles/PMC3125358/ /pubmed/21651801 http://dx.doi.org/10.1186/1743-422X-8-284 Text en Copyright ©2011 Cervantes-Garcia et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Report Cervantes-Garcia, Daniel Rojas-Martinez, Augusto Camerini, David An XMRV Derived Retroviral Vector as a Tool for Gene Transfer |
title | An XMRV Derived Retroviral Vector as a Tool for Gene Transfer |
title_full | An XMRV Derived Retroviral Vector as a Tool for Gene Transfer |
title_fullStr | An XMRV Derived Retroviral Vector as a Tool for Gene Transfer |
title_full_unstemmed | An XMRV Derived Retroviral Vector as a Tool for Gene Transfer |
title_short | An XMRV Derived Retroviral Vector as a Tool for Gene Transfer |
title_sort | xmrv derived retroviral vector as a tool for gene transfer |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3125358/ https://www.ncbi.nlm.nih.gov/pubmed/21651801 http://dx.doi.org/10.1186/1743-422X-8-284 |
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