MultiFit: a web server for fitting multiple protein structures into their electron microscopy density map

Advances in electron microscopy (EM) allow for structure determination of large biological assemblies at increasingly higher resolutions. A key step in this process is fitting multiple component structures into an EM-derived density map of their assembly. Here, we describe a web server for this task...

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Detalles Bibliográficos
Autores principales: Tjioe, Elina, Lasker, Keren, Webb, Ben, Wolfson, Haim J., Sali, Andrej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3125811/
https://www.ncbi.nlm.nih.gov/pubmed/21715383
http://dx.doi.org/10.1093/nar/gkr490
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author Tjioe, Elina
Lasker, Keren
Webb, Ben
Wolfson, Haim J.
Sali, Andrej
author_facet Tjioe, Elina
Lasker, Keren
Webb, Ben
Wolfson, Haim J.
Sali, Andrej
author_sort Tjioe, Elina
collection PubMed
description Advances in electron microscopy (EM) allow for structure determination of large biological assemblies at increasingly higher resolutions. A key step in this process is fitting multiple component structures into an EM-derived density map of their assembly. Here, we describe a web server for this task. The server takes as input a set of protein structures in the PDB format and an EM density map in the MRC format. The output is an ensemble of models ranked by their quality of fit to the density map. The models can be viewed online or downloaded from the website. The service is available at; http://salilab.org/multifit/ and http://bioinfo3d.cs.tau.ac.il/.
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spelling pubmed-31258112011-07-05 MultiFit: a web server for fitting multiple protein structures into their electron microscopy density map Tjioe, Elina Lasker, Keren Webb, Ben Wolfson, Haim J. Sali, Andrej Nucleic Acids Res Articles Advances in electron microscopy (EM) allow for structure determination of large biological assemblies at increasingly higher resolutions. A key step in this process is fitting multiple component structures into an EM-derived density map of their assembly. Here, we describe a web server for this task. The server takes as input a set of protein structures in the PDB format and an EM density map in the MRC format. The output is an ensemble of models ranked by their quality of fit to the density map. The models can be viewed online or downloaded from the website. The service is available at; http://salilab.org/multifit/ and http://bioinfo3d.cs.tau.ac.il/. Oxford University Press 2011-07-01 2011-06-27 /pmc/articles/PMC3125811/ /pubmed/21715383 http://dx.doi.org/10.1093/nar/gkr490 Text en © The Author(s) 2011. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Tjioe, Elina
Lasker, Keren
Webb, Ben
Wolfson, Haim J.
Sali, Andrej
MultiFit: a web server for fitting multiple protein structures into their electron microscopy density map
title MultiFit: a web server for fitting multiple protein structures into their electron microscopy density map
title_full MultiFit: a web server for fitting multiple protein structures into their electron microscopy density map
title_fullStr MultiFit: a web server for fitting multiple protein structures into their electron microscopy density map
title_full_unstemmed MultiFit: a web server for fitting multiple protein structures into their electron microscopy density map
title_short MultiFit: a web server for fitting multiple protein structures into their electron microscopy density map
title_sort multifit: a web server for fitting multiple protein structures into their electron microscopy density map
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3125811/
https://www.ncbi.nlm.nih.gov/pubmed/21715383
http://dx.doi.org/10.1093/nar/gkr490
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AT wolfsonhaimj multifitawebserverforfittingmultipleproteinstructuresintotheirelectronmicroscopydensitymap
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