A Sequential Strand-Displacement Strategy Enables Efficient Six-Step DNA-Templated Synthesis

[Image: see text] We developed a sequential strand-displacement strategy for multistep DNA-templated synthesis (DTS) and used it to mediate an efficient six-step DTS that proceeded in 35% overall yield (83% average yield per step). The efficiency of this approach and the fact that the final product...

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Detalles Bibliográficos
Autores principales: He, Yu, Liu, David R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2011
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3125949/
https://www.ncbi.nlm.nih.gov/pubmed/21657248
http://dx.doi.org/10.1021/ja201361t
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author He, Yu
Liu, David R.
author_facet He, Yu
Liu, David R.
author_sort He, Yu
collection PubMed
description [Image: see text] We developed a sequential strand-displacement strategy for multistep DNA-templated synthesis (DTS) and used it to mediate an efficient six-step DTS that proceeded in 35% overall yield (83% average yield per step). The efficiency of this approach and the fact that the final product remains linked to a DNA sequence that fully encodes its reaction history suggests its utility for the translation of DNA sequences into high-complexity synthetic libraries suitable for in vitro selection.
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spelling pubmed-31259492011-06-29 A Sequential Strand-Displacement Strategy Enables Efficient Six-Step DNA-Templated Synthesis He, Yu Liu, David R. J Am Chem Soc [Image: see text] We developed a sequential strand-displacement strategy for multistep DNA-templated synthesis (DTS) and used it to mediate an efficient six-step DTS that proceeded in 35% overall yield (83% average yield per step). The efficiency of this approach and the fact that the final product remains linked to a DNA sequence that fully encodes its reaction history suggests its utility for the translation of DNA sequences into high-complexity synthetic libraries suitable for in vitro selection. American Chemical Society 2011-06-09 2011-07-06 /pmc/articles/PMC3125949/ /pubmed/21657248 http://dx.doi.org/10.1021/ja201361t Text en Copyright © 2011 American Chemical Society http://pubs.acs.org This is an open-access article distributed under the ACS AuthorChoice Terms & Conditions. Any use of this article, must conform to the terms of that license which are available at http://pubs.acs.org.
spellingShingle He, Yu
Liu, David R.
A Sequential Strand-Displacement Strategy Enables Efficient Six-Step DNA-Templated Synthesis
title A Sequential Strand-Displacement Strategy Enables Efficient Six-Step DNA-Templated Synthesis
title_full A Sequential Strand-Displacement Strategy Enables Efficient Six-Step DNA-Templated Synthesis
title_fullStr A Sequential Strand-Displacement Strategy Enables Efficient Six-Step DNA-Templated Synthesis
title_full_unstemmed A Sequential Strand-Displacement Strategy Enables Efficient Six-Step DNA-Templated Synthesis
title_short A Sequential Strand-Displacement Strategy Enables Efficient Six-Step DNA-Templated Synthesis
title_sort sequential strand-displacement strategy enables efficient six-step dna-templated synthesis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3125949/
https://www.ncbi.nlm.nih.gov/pubmed/21657248
http://dx.doi.org/10.1021/ja201361t
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