Highly efficient PCR assay to discriminate allelic DNA methylation status using whole genome amplification

BACKGROUND: We previously developed a simple method termed HpaII-McrBC PCR (HM-PCR) to discriminate allelic methylation status of the genomic sites of interest, and successfully applied it to a comprehensive analysis of CpG islands (CGIs) on human chromosome 21q. However, HM-PCR requires 200 ng of g...

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Detalles Bibliográficos
Autores principales: Yamada, Yoichi, Ito, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3127961/
https://www.ncbi.nlm.nih.gov/pubmed/21663670
http://dx.doi.org/10.1186/1756-0500-4-179

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3127961/
https://www.ncbi.nlm.nih.gov/pubmed/21663670
http://dx.doi.org/10.1186/1756-0500-4-179

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