Cargando…
Highly efficient PCR assay to discriminate allelic DNA methylation status using whole genome amplification
BACKGROUND: We previously developed a simple method termed HpaII-McrBC PCR (HM-PCR) to discriminate allelic methylation status of the genomic sites of interest, and successfully applied it to a comprehensive analysis of CpG islands (CGIs) on human chromosome 21q. However, HM-PCR requires 200 ng of g...
| Autores principales: | , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2011
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3127961/ https://www.ncbi.nlm.nih.gov/pubmed/21663670 http://dx.doi.org/10.1186/1756-0500-4-179 |