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A Facile Nanoparticle Immunoassay for Cancer Biomarker Discovery

BACKGROUND: Gold nanoparticles (AuNPs) scatter light intensely at or near their surface plasmon wavelength region. Using AuNPs coupled with dynamic light scattering (DLS) detection, we developed a facile nanoparticle immunoassay for serum protein biomarker detection and analysis. A serum sample was...

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Detalles Bibliográficos
Autores principales: Huo, Qun, Colon, Jimmie, Cordero, Adam, Bogdanovic, Jelena, Baker, Cheryl H, Goodison, Steven, Pensky, Marianna Y
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3127990/
https://www.ncbi.nlm.nih.gov/pubmed/21605409
http://dx.doi.org/10.1186/1477-3155-9-20
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author Huo, Qun
Colon, Jimmie
Cordero, Adam
Bogdanovic, Jelena
Baker, Cheryl H
Goodison, Steven
Pensky, Marianna Y
author_facet Huo, Qun
Colon, Jimmie
Cordero, Adam
Bogdanovic, Jelena
Baker, Cheryl H
Goodison, Steven
Pensky, Marianna Y
author_sort Huo, Qun
collection PubMed
description BACKGROUND: Gold nanoparticles (AuNPs) scatter light intensely at or near their surface plasmon wavelength region. Using AuNPs coupled with dynamic light scattering (DLS) detection, we developed a facile nanoparticle immunoassay for serum protein biomarker detection and analysis. A serum sample was first mixed with a citrate-protected AuNP solution. Proteins from the serum were adsorbed to the AuNPs to form a protein corona on the nanoparticle surface. An antibody solution was then added to the assay solution to analyze the target proteins of interest that are present in the protein corona. The protein corona formation and the subsequent binding of antibody to the target proteins in the protein corona were detected by DLS. RESULTS: Using this simple assay, we discovered multiple molecular aberrations associated with prostate cancer from both mice and human blood serum samples. From the mice serum study, we observed difference in the size of the protein corona and mouse IgG level between different mice groups (i.e., mice with aggressive or less aggressive prostate cancer, and normal healthy controls). Furthermore, it was found from both the mice model and the human serum sample study that the level of vascular endothelial growth factor (VEGF, a protein that is associated with tumor angiogenesis) adsorbed to the AuNPs is decreased in cancer samples compared to non-cancerous or less malignant cancer samples. CONCLUSION: The molecular aberrations observed from this study may become new biomarkers for prostate cancer detection. The nanoparticle immunoassay reported here can be used as a convenient and general tool to screen and analyze serum proteins and to discover new biomarkers associated with cancer and other human diseases.
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spelling pubmed-31279902011-07-01 A Facile Nanoparticle Immunoassay for Cancer Biomarker Discovery Huo, Qun Colon, Jimmie Cordero, Adam Bogdanovic, Jelena Baker, Cheryl H Goodison, Steven Pensky, Marianna Y J Nanobiotechnology Research BACKGROUND: Gold nanoparticles (AuNPs) scatter light intensely at or near their surface plasmon wavelength region. Using AuNPs coupled with dynamic light scattering (DLS) detection, we developed a facile nanoparticle immunoassay for serum protein biomarker detection and analysis. A serum sample was first mixed with a citrate-protected AuNP solution. Proteins from the serum were adsorbed to the AuNPs to form a protein corona on the nanoparticle surface. An antibody solution was then added to the assay solution to analyze the target proteins of interest that are present in the protein corona. The protein corona formation and the subsequent binding of antibody to the target proteins in the protein corona were detected by DLS. RESULTS: Using this simple assay, we discovered multiple molecular aberrations associated with prostate cancer from both mice and human blood serum samples. From the mice serum study, we observed difference in the size of the protein corona and mouse IgG level between different mice groups (i.e., mice with aggressive or less aggressive prostate cancer, and normal healthy controls). Furthermore, it was found from both the mice model and the human serum sample study that the level of vascular endothelial growth factor (VEGF, a protein that is associated with tumor angiogenesis) adsorbed to the AuNPs is decreased in cancer samples compared to non-cancerous or less malignant cancer samples. CONCLUSION: The molecular aberrations observed from this study may become new biomarkers for prostate cancer detection. The nanoparticle immunoassay reported here can be used as a convenient and general tool to screen and analyze serum proteins and to discover new biomarkers associated with cancer and other human diseases. BioMed Central 2011-05-23 /pmc/articles/PMC3127990/ /pubmed/21605409 http://dx.doi.org/10.1186/1477-3155-9-20 Text en Copyright ©2011 Huo et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Huo, Qun
Colon, Jimmie
Cordero, Adam
Bogdanovic, Jelena
Baker, Cheryl H
Goodison, Steven
Pensky, Marianna Y
A Facile Nanoparticle Immunoassay for Cancer Biomarker Discovery
title A Facile Nanoparticle Immunoassay for Cancer Biomarker Discovery
title_full A Facile Nanoparticle Immunoassay for Cancer Biomarker Discovery
title_fullStr A Facile Nanoparticle Immunoassay for Cancer Biomarker Discovery
title_full_unstemmed A Facile Nanoparticle Immunoassay for Cancer Biomarker Discovery
title_short A Facile Nanoparticle Immunoassay for Cancer Biomarker Discovery
title_sort facile nanoparticle immunoassay for cancer biomarker discovery
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3127990/
https://www.ncbi.nlm.nih.gov/pubmed/21605409
http://dx.doi.org/10.1186/1477-3155-9-20
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