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Identification and Characterization of NF-Y Transcription Factor Families in the Monocot Model Plant Brachypodium distachyon

BACKGROUND: Nuclear Factor Y (NF-Y) is a heterotrimeric transcription factor composed of NF-YA, NF-YB and NF-YC proteins. Using the dicot plant model system Arabidopsis thaliana (Arabidopsis), NF-Y were previously shown to control a variety of agronomically important traits, including drought tolera...

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Autores principales: Cao, Shuanghe, Kumimoto, Roderick W., Siriwardana, Chamindika L., Risinger, Jan R., Holt, Ben F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3128097/
https://www.ncbi.nlm.nih.gov/pubmed/21738795
http://dx.doi.org/10.1371/journal.pone.0021805
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author Cao, Shuanghe
Kumimoto, Roderick W.
Siriwardana, Chamindika L.
Risinger, Jan R.
Holt, Ben F.
author_facet Cao, Shuanghe
Kumimoto, Roderick W.
Siriwardana, Chamindika L.
Risinger, Jan R.
Holt, Ben F.
author_sort Cao, Shuanghe
collection PubMed
description BACKGROUND: Nuclear Factor Y (NF-Y) is a heterotrimeric transcription factor composed of NF-YA, NF-YB and NF-YC proteins. Using the dicot plant model system Arabidopsis thaliana (Arabidopsis), NF-Y were previously shown to control a variety of agronomically important traits, including drought tolerance, flowering time, and seed development. The aim of the current research was to identify and characterize NF-Y families in the emerging monocot model plant Brachypodium distachyon (Brachypodium) with the long term goal of assisting in the translation of known dicot NF-Y functions to the grasses. METHODOLOGY/PRINCIPAL FINDINGS: We identified, annotated, and further characterized 7 NF-YA, 17 NF-YB, and 12 NF-YC proteins in Brachypodium (BdNF-Y). By examining phylogenetic relationships, orthology predictions, and tissue-specific expression patterns for all 36 BdNF-Y, we proposed numerous examples of likely functional conservation between dicots and monocots. To test one of these orthology predictions, we demonstrated that a BdNF-YB with predicted orthology to Arabidopsis floral-promoting NF-Y proteins can rescue a late flowering Arabidopsis mutant. CONCLUSIONS/SIGNIFICANCE: The Brachypodium genome encodes a similar complement of NF-Y to other sequenced angiosperms. Information regarding NF-Y phylogenetic relationships, predicted orthologies, and expression patterns can facilitate their study in the grasses. The current data serves as an entry point for translating many NF-Y functions from dicots to the genetically tractable monocot model system Brachypodium. In turn, studies of NF-Y function in Brachypodium promise to be more readily translatable to the agriculturally important grasses.
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spelling pubmed-31280972011-07-07 Identification and Characterization of NF-Y Transcription Factor Families in the Monocot Model Plant Brachypodium distachyon Cao, Shuanghe Kumimoto, Roderick W. Siriwardana, Chamindika L. Risinger, Jan R. Holt, Ben F. PLoS One Research Article BACKGROUND: Nuclear Factor Y (NF-Y) is a heterotrimeric transcription factor composed of NF-YA, NF-YB and NF-YC proteins. Using the dicot plant model system Arabidopsis thaliana (Arabidopsis), NF-Y were previously shown to control a variety of agronomically important traits, including drought tolerance, flowering time, and seed development. The aim of the current research was to identify and characterize NF-Y families in the emerging monocot model plant Brachypodium distachyon (Brachypodium) with the long term goal of assisting in the translation of known dicot NF-Y functions to the grasses. METHODOLOGY/PRINCIPAL FINDINGS: We identified, annotated, and further characterized 7 NF-YA, 17 NF-YB, and 12 NF-YC proteins in Brachypodium (BdNF-Y). By examining phylogenetic relationships, orthology predictions, and tissue-specific expression patterns for all 36 BdNF-Y, we proposed numerous examples of likely functional conservation between dicots and monocots. To test one of these orthology predictions, we demonstrated that a BdNF-YB with predicted orthology to Arabidopsis floral-promoting NF-Y proteins can rescue a late flowering Arabidopsis mutant. CONCLUSIONS/SIGNIFICANCE: The Brachypodium genome encodes a similar complement of NF-Y to other sequenced angiosperms. Information regarding NF-Y phylogenetic relationships, predicted orthologies, and expression patterns can facilitate their study in the grasses. The current data serves as an entry point for translating many NF-Y functions from dicots to the genetically tractable monocot model system Brachypodium. In turn, studies of NF-Y function in Brachypodium promise to be more readily translatable to the agriculturally important grasses. Public Library of Science 2011-06-30 /pmc/articles/PMC3128097/ /pubmed/21738795 http://dx.doi.org/10.1371/journal.pone.0021805 Text en Cao et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Cao, Shuanghe
Kumimoto, Roderick W.
Siriwardana, Chamindika L.
Risinger, Jan R.
Holt, Ben F.
Identification and Characterization of NF-Y Transcription Factor Families in the Monocot Model Plant Brachypodium distachyon
title Identification and Characterization of NF-Y Transcription Factor Families in the Monocot Model Plant Brachypodium distachyon
title_full Identification and Characterization of NF-Y Transcription Factor Families in the Monocot Model Plant Brachypodium distachyon
title_fullStr Identification and Characterization of NF-Y Transcription Factor Families in the Monocot Model Plant Brachypodium distachyon
title_full_unstemmed Identification and Characterization of NF-Y Transcription Factor Families in the Monocot Model Plant Brachypodium distachyon
title_short Identification and Characterization of NF-Y Transcription Factor Families in the Monocot Model Plant Brachypodium distachyon
title_sort identification and characterization of nf-y transcription factor families in the monocot model plant brachypodium distachyon
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3128097/
https://www.ncbi.nlm.nih.gov/pubmed/21738795
http://dx.doi.org/10.1371/journal.pone.0021805
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