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Lansoprazole inhibits mitochondrial superoxide production and cellular lipid peroxidation induced by indomethacin in RGM1 cells

Lansoprazole is effective in healing non-steroidal anti-inflammatory drugs induced ulcers, and antioxidant properties have been thought to play a key role in healing ulcers. We hypothesize that lansoprazole exerts a cytoprotective effect by inhibiting reactive oxygen species leakage from mitochondri...

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Autores principales: Rai, Kanho, Matsui, Hirofumi, Kaneko, Tsuyoshi, Nagano, Yumiko, Shimokawa, Osamu, Udo, Jumpei, Hirayama, Aki, Hyodo, Ichinosuke, Indo, Hiroko P., Majima, Hideyuki J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Society for Free Radical Research Japan 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3128362/
https://www.ncbi.nlm.nih.gov/pubmed/21765603
http://dx.doi.org/10.3164/jcbn.10-133
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author Rai, Kanho
Matsui, Hirofumi
Kaneko, Tsuyoshi
Nagano, Yumiko
Shimokawa, Osamu
Udo, Jumpei
Hirayama, Aki
Hyodo, Ichinosuke
Indo, Hiroko P.
Majima, Hideyuki J.
author_facet Rai, Kanho
Matsui, Hirofumi
Kaneko, Tsuyoshi
Nagano, Yumiko
Shimokawa, Osamu
Udo, Jumpei
Hirayama, Aki
Hyodo, Ichinosuke
Indo, Hiroko P.
Majima, Hideyuki J.
author_sort Rai, Kanho
collection PubMed
description Lansoprazole is effective in healing non-steroidal anti-inflammatory drugs induced ulcers, and antioxidant properties have been thought to play a key role in healing ulcers. We hypothesize that lansoprazole exerts a cytoprotective effect by inhibiting reactive oxygen species leakage from mitochondria and lipid peroxidation. We pretreated gastric epithelial RGM1 cells with lansoprazole and then treated them with indomethacin in vitro. We found that the lansoprazole pretreatment significantly reduced cellular injury, maintained mitochondrial transmembrane potential, and decreased lipid peroxidation. Furthermore, the signal intensity of the electron spin resonance spectrum of the indomethacin-treated mitochondria which were pretreated with lansoprazole showed considerable reduction compared to those without the lansoprazole pretreatment. These results suggest that lansoprazole reduced superoxide production in the mitochondria of indomethacin treated cells, and subsequently inhibited lipid peroxide and cellular injury in gastric epithelial cells.
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spelling pubmed-31283622011-07-15 Lansoprazole inhibits mitochondrial superoxide production and cellular lipid peroxidation induced by indomethacin in RGM1 cells Rai, Kanho Matsui, Hirofumi Kaneko, Tsuyoshi Nagano, Yumiko Shimokawa, Osamu Udo, Jumpei Hirayama, Aki Hyodo, Ichinosuke Indo, Hiroko P. Majima, Hideyuki J. J Clin Biochem Nutr Original Article Lansoprazole is effective in healing non-steroidal anti-inflammatory drugs induced ulcers, and antioxidant properties have been thought to play a key role in healing ulcers. We hypothesize that lansoprazole exerts a cytoprotective effect by inhibiting reactive oxygen species leakage from mitochondria and lipid peroxidation. We pretreated gastric epithelial RGM1 cells with lansoprazole and then treated them with indomethacin in vitro. We found that the lansoprazole pretreatment significantly reduced cellular injury, maintained mitochondrial transmembrane potential, and decreased lipid peroxidation. Furthermore, the signal intensity of the electron spin resonance spectrum of the indomethacin-treated mitochondria which were pretreated with lansoprazole showed considerable reduction compared to those without the lansoprazole pretreatment. These results suggest that lansoprazole reduced superoxide production in the mitochondria of indomethacin treated cells, and subsequently inhibited lipid peroxide and cellular injury in gastric epithelial cells. the Society for Free Radical Research Japan 2011-07 2011-06-17 /pmc/articles/PMC3128362/ /pubmed/21765603 http://dx.doi.org/10.3164/jcbn.10-133 Text en Copyright © 2011 JCBN This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Rai, Kanho
Matsui, Hirofumi
Kaneko, Tsuyoshi
Nagano, Yumiko
Shimokawa, Osamu
Udo, Jumpei
Hirayama, Aki
Hyodo, Ichinosuke
Indo, Hiroko P.
Majima, Hideyuki J.
Lansoprazole inhibits mitochondrial superoxide production and cellular lipid peroxidation induced by indomethacin in RGM1 cells
title Lansoprazole inhibits mitochondrial superoxide production and cellular lipid peroxidation induced by indomethacin in RGM1 cells
title_full Lansoprazole inhibits mitochondrial superoxide production and cellular lipid peroxidation induced by indomethacin in RGM1 cells
title_fullStr Lansoprazole inhibits mitochondrial superoxide production and cellular lipid peroxidation induced by indomethacin in RGM1 cells
title_full_unstemmed Lansoprazole inhibits mitochondrial superoxide production and cellular lipid peroxidation induced by indomethacin in RGM1 cells
title_short Lansoprazole inhibits mitochondrial superoxide production and cellular lipid peroxidation induced by indomethacin in RGM1 cells
title_sort lansoprazole inhibits mitochondrial superoxide production and cellular lipid peroxidation induced by indomethacin in rgm1 cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3128362/
https://www.ncbi.nlm.nih.gov/pubmed/21765603
http://dx.doi.org/10.3164/jcbn.10-133
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