Cargando…

A nuclear factor-κB signaling pathway via protein kinase C δ regulates replication of respiratory syncytial virus in polarized normal human nasal epithelial cells

Respiratory syncytial virus (RSV) is the major cause of bronchitis, asthma, and severe lower respiratory tract disease in infants and young children. The airway epithelium, which has a well-developed barrier regulated by tight junctions, is the first line of defense during respiratory virus infectio...

Descripción completa

Detalles Bibliográficos
Autores principales: Masaki, Tomoyuki, Kojima, Takashi, Okabayashi, Tamaki, Ogasawara, Noriko, Ohkuni, Tsuyoshi, Obata, Kazufumi, Takasawa, Akira, Murata, Masaki, Tanaka, Satoshi, Hirakawa, Satoshi, Fuchimoto, Jun, Ninomiya, Takafumi, Fujii, Nobuhiro, Tsutsumi, Hiroyuki, Himi, Tetsuo, Sawada, Norimasa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3128518/
https://www.ncbi.nlm.nih.gov/pubmed/21562222
http://dx.doi.org/10.1091/mbc.E10-11-0875
_version_ 1782207443975012352
author Masaki, Tomoyuki
Kojima, Takashi
Okabayashi, Tamaki
Ogasawara, Noriko
Ohkuni, Tsuyoshi
Obata, Kazufumi
Takasawa, Akira
Murata, Masaki
Tanaka, Satoshi
Hirakawa, Satoshi
Fuchimoto, Jun
Ninomiya, Takafumi
Fujii, Nobuhiro
Tsutsumi, Hiroyuki
Himi, Tetsuo
Sawada, Norimasa
author_facet Masaki, Tomoyuki
Kojima, Takashi
Okabayashi, Tamaki
Ogasawara, Noriko
Ohkuni, Tsuyoshi
Obata, Kazufumi
Takasawa, Akira
Murata, Masaki
Tanaka, Satoshi
Hirakawa, Satoshi
Fuchimoto, Jun
Ninomiya, Takafumi
Fujii, Nobuhiro
Tsutsumi, Hiroyuki
Himi, Tetsuo
Sawada, Norimasa
author_sort Masaki, Tomoyuki
collection PubMed
description Respiratory syncytial virus (RSV) is the major cause of bronchitis, asthma, and severe lower respiratory tract disease in infants and young children. The airway epithelium, which has a well-developed barrier regulated by tight junctions, is the first line of defense during respiratory virus infection. In upper airway human nasal epithelial cells (HNECs), however, the primary site of RSV infection, the mechanisms of replication and budding of RSV, and the epithelial cell responses, including the tight junctional barrier, remain unknown. To investigate the detailed mechanisms of replication and budding of RSV in HNECs and the epithelial cell responses, we established an RSV-infected model using human telomerase reverse transcriptase–-transfected HNECs. We first found that the expression and barrier function of tight junction molecules claudin-4 and occludin were markedly induced together with production of proinflammatory cytokines interleukin 8 and tumor necrosis factor-α in HNECs after RSV infection, and the induction of tight junction molecules possibly contributed to budding of RSV. Furthermore, the replication and budding of RSV and the epithelial cell responses in HNECs were regulated via a protein kinase C δ/hypoxia-inducible factor-1α/nuclear factor-κB pathway. The control of this pathway in HNECs may be useful not only for prevention of replication and budding of RSV, but also in therapy for RSV-induced respiratory pathogenesis.
format Online
Article
Text
id pubmed-3128518
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher The American Society for Cell Biology
record_format MEDLINE/PubMed
spelling pubmed-31285182011-09-16 A nuclear factor-κB signaling pathway via protein kinase C δ regulates replication of respiratory syncytial virus in polarized normal human nasal epithelial cells Masaki, Tomoyuki Kojima, Takashi Okabayashi, Tamaki Ogasawara, Noriko Ohkuni, Tsuyoshi Obata, Kazufumi Takasawa, Akira Murata, Masaki Tanaka, Satoshi Hirakawa, Satoshi Fuchimoto, Jun Ninomiya, Takafumi Fujii, Nobuhiro Tsutsumi, Hiroyuki Himi, Tetsuo Sawada, Norimasa Mol Biol Cell Articles Respiratory syncytial virus (RSV) is the major cause of bronchitis, asthma, and severe lower respiratory tract disease in infants and young children. The airway epithelium, which has a well-developed barrier regulated by tight junctions, is the first line of defense during respiratory virus infection. In upper airway human nasal epithelial cells (HNECs), however, the primary site of RSV infection, the mechanisms of replication and budding of RSV, and the epithelial cell responses, including the tight junctional barrier, remain unknown. To investigate the detailed mechanisms of replication and budding of RSV in HNECs and the epithelial cell responses, we established an RSV-infected model using human telomerase reverse transcriptase–-transfected HNECs. We first found that the expression and barrier function of tight junction molecules claudin-4 and occludin were markedly induced together with production of proinflammatory cytokines interleukin 8 and tumor necrosis factor-α in HNECs after RSV infection, and the induction of tight junction molecules possibly contributed to budding of RSV. Furthermore, the replication and budding of RSV and the epithelial cell responses in HNECs were regulated via a protein kinase C δ/hypoxia-inducible factor-1α/nuclear factor-κB pathway. The control of this pathway in HNECs may be useful not only for prevention of replication and budding of RSV, but also in therapy for RSV-induced respiratory pathogenesis. The American Society for Cell Biology 2011-07-01 /pmc/articles/PMC3128518/ /pubmed/21562222 http://dx.doi.org/10.1091/mbc.E10-11-0875 Text en © 2011 Masaki et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Masaki, Tomoyuki
Kojima, Takashi
Okabayashi, Tamaki
Ogasawara, Noriko
Ohkuni, Tsuyoshi
Obata, Kazufumi
Takasawa, Akira
Murata, Masaki
Tanaka, Satoshi
Hirakawa, Satoshi
Fuchimoto, Jun
Ninomiya, Takafumi
Fujii, Nobuhiro
Tsutsumi, Hiroyuki
Himi, Tetsuo
Sawada, Norimasa
A nuclear factor-κB signaling pathway via protein kinase C δ regulates replication of respiratory syncytial virus in polarized normal human nasal epithelial cells
title A nuclear factor-κB signaling pathway via protein kinase C δ regulates replication of respiratory syncytial virus in polarized normal human nasal epithelial cells
title_full A nuclear factor-κB signaling pathway via protein kinase C δ regulates replication of respiratory syncytial virus in polarized normal human nasal epithelial cells
title_fullStr A nuclear factor-κB signaling pathway via protein kinase C δ regulates replication of respiratory syncytial virus in polarized normal human nasal epithelial cells
title_full_unstemmed A nuclear factor-κB signaling pathway via protein kinase C δ regulates replication of respiratory syncytial virus in polarized normal human nasal epithelial cells
title_short A nuclear factor-κB signaling pathway via protein kinase C δ regulates replication of respiratory syncytial virus in polarized normal human nasal epithelial cells
title_sort nuclear factor-κb signaling pathway via protein kinase c δ regulates replication of respiratory syncytial virus in polarized normal human nasal epithelial cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3128518/
https://www.ncbi.nlm.nih.gov/pubmed/21562222
http://dx.doi.org/10.1091/mbc.E10-11-0875
work_keys_str_mv AT masakitomoyuki anuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT kojimatakashi anuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT okabayashitamaki anuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT ogasawaranoriko anuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT ohkunitsuyoshi anuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT obatakazufumi anuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT takasawaakira anuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT muratamasaki anuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT tanakasatoshi anuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT hirakawasatoshi anuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT fuchimotojun anuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT ninomiyatakafumi anuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT fujiinobuhiro anuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT tsutsumihiroyuki anuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT himitetsuo anuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT sawadanorimasa anuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT masakitomoyuki nuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT kojimatakashi nuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT okabayashitamaki nuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT ogasawaranoriko nuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT ohkunitsuyoshi nuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT obatakazufumi nuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT takasawaakira nuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT muratamasaki nuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT tanakasatoshi nuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT hirakawasatoshi nuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT fuchimotojun nuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT ninomiyatakafumi nuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT fujiinobuhiro nuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT tsutsumihiroyuki nuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT himitetsuo nuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells
AT sawadanorimasa nuclearfactorkbsignalingpathwayviaproteinkinasecdregulatesreplicationofrespiratorysyncytialvirusinpolarizednormalhumannasalepithelialcells