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Trabecular Meshwork Gene Expression after Selective Laser Trabeculoplasty

BACKGROUND: Trabecular meshwork and Schlemm's canal are the tissues appointed to modulate the aqueous humour outflow from the anterior chamber. The impairment of their functions drives to an intraocular pressure increase. The selective laser trabeculoplasty is a laser therapy of the trabecular...

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Autores principales: Izzotti, Alberto, Longobardi, Mariagrazia, Cartiglia, Cristina, Rathschuler, Federico, Saccà, Sergio Claudio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3128580/
https://www.ncbi.nlm.nih.gov/pubmed/21747927
http://dx.doi.org/10.1371/journal.pone.0020110
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author Izzotti, Alberto
Longobardi, Mariagrazia
Cartiglia, Cristina
Rathschuler, Federico
Saccà, Sergio Claudio
author_facet Izzotti, Alberto
Longobardi, Mariagrazia
Cartiglia, Cristina
Rathschuler, Federico
Saccà, Sergio Claudio
author_sort Izzotti, Alberto
collection PubMed
description BACKGROUND: Trabecular meshwork and Schlemm's canal are the tissues appointed to modulate the aqueous humour outflow from the anterior chamber. The impairment of their functions drives to an intraocular pressure increase. The selective laser trabeculoplasty is a laser therapy of the trabecular meshwork able to decrease intraocular pressure. The exact response mechanism to this treatment has not been clearly delineated yet. The herein presented study is aimed at studying the gene expression changes induced in trabecular meshwork cells by selective laser trabeculoplasty (SLT) in order to better understand the mechanisms subtending its efficacy. METHODOLOGY/PRINCIPAL FINDINGS: Primary human trabecular meshwork cells cultured in fibroblast medium underwent selective laser trabeculoplasty treatment. RNA was extracted from a pool of cells 30 minutes after treatment while the remaining cells were further cultured and RNA was extracted respectively 2 and 6 hours after treatment. Control cells stored in incubator in absence of SLT treatment were used as reference samples. Gene expression was evaluated by hybridization on miRNA-microarray and laser scanner analysis. Scanning electron microscopic examination was performed on 2 Trabecular meshwork samples after SLT at 4(th) and 6(th) hour from treatment. On the whole, selective laser trabeculoplasty modulates in trabecular meshwork the expression of genes involved in cell motility, intercellular connections, extracellular matrix production, protein repair, DNA repair, membrane repair, reactive oxygen species production, glutamate toxicity, antioxidant activities, and inflammation. CONCLUSIONS/SIGNIFICANCE: SLT did not induce any phenotypic alteration in TM samples. TM is a complex tissue possessing a great variety of function pivotal for the active regulation of aqueous humour outflow from the anterior chamber. SLT is able to modulate these functions at the postgenomic molecular level without inducing damage either at molecular or phenotypic levels.
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spelling pubmed-31285802011-07-11 Trabecular Meshwork Gene Expression after Selective Laser Trabeculoplasty Izzotti, Alberto Longobardi, Mariagrazia Cartiglia, Cristina Rathschuler, Federico Saccà, Sergio Claudio PLoS One Research Article BACKGROUND: Trabecular meshwork and Schlemm's canal are the tissues appointed to modulate the aqueous humour outflow from the anterior chamber. The impairment of their functions drives to an intraocular pressure increase. The selective laser trabeculoplasty is a laser therapy of the trabecular meshwork able to decrease intraocular pressure. The exact response mechanism to this treatment has not been clearly delineated yet. The herein presented study is aimed at studying the gene expression changes induced in trabecular meshwork cells by selective laser trabeculoplasty (SLT) in order to better understand the mechanisms subtending its efficacy. METHODOLOGY/PRINCIPAL FINDINGS: Primary human trabecular meshwork cells cultured in fibroblast medium underwent selective laser trabeculoplasty treatment. RNA was extracted from a pool of cells 30 minutes after treatment while the remaining cells were further cultured and RNA was extracted respectively 2 and 6 hours after treatment. Control cells stored in incubator in absence of SLT treatment were used as reference samples. Gene expression was evaluated by hybridization on miRNA-microarray and laser scanner analysis. Scanning electron microscopic examination was performed on 2 Trabecular meshwork samples after SLT at 4(th) and 6(th) hour from treatment. On the whole, selective laser trabeculoplasty modulates in trabecular meshwork the expression of genes involved in cell motility, intercellular connections, extracellular matrix production, protein repair, DNA repair, membrane repair, reactive oxygen species production, glutamate toxicity, antioxidant activities, and inflammation. CONCLUSIONS/SIGNIFICANCE: SLT did not induce any phenotypic alteration in TM samples. TM is a complex tissue possessing a great variety of function pivotal for the active regulation of aqueous humour outflow from the anterior chamber. SLT is able to modulate these functions at the postgenomic molecular level without inducing damage either at molecular or phenotypic levels. Public Library of Science 2011-07-01 /pmc/articles/PMC3128580/ /pubmed/21747927 http://dx.doi.org/10.1371/journal.pone.0020110 Text en Izzotti et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Izzotti, Alberto
Longobardi, Mariagrazia
Cartiglia, Cristina
Rathschuler, Federico
Saccà, Sergio Claudio
Trabecular Meshwork Gene Expression after Selective Laser Trabeculoplasty
title Trabecular Meshwork Gene Expression after Selective Laser Trabeculoplasty
title_full Trabecular Meshwork Gene Expression after Selective Laser Trabeculoplasty
title_fullStr Trabecular Meshwork Gene Expression after Selective Laser Trabeculoplasty
title_full_unstemmed Trabecular Meshwork Gene Expression after Selective Laser Trabeculoplasty
title_short Trabecular Meshwork Gene Expression after Selective Laser Trabeculoplasty
title_sort trabecular meshwork gene expression after selective laser trabeculoplasty
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3128580/
https://www.ncbi.nlm.nih.gov/pubmed/21747927
http://dx.doi.org/10.1371/journal.pone.0020110
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