Cargando…

Effects of Baicalin on inflammatory mediators and pancreatic acinar cell apoptosis in rats with sever acute pancreatitis

BACKGROUND: To investigate the effects of Baicalin and Octreotide on inflammatory mediators and pancreatic acinar cells apoptosis of rats with severe acute pancreatitis (SAP). METHODS: SD rats were randomly divided into sham operated group (I group), model control group (II group), Baicalin treated...

Descripción completa

Detalles Bibliográficos
Autores principales: Xiping, Zhang, Hua, Tian, Hanqing, Chen, Li, Chen, Binyan, Yu, Jing, Ma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications Pvt Ltd 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3129065/
https://www.ncbi.nlm.nih.gov/pubmed/21772857
_version_ 1782207507972751360
author Xiping, Zhang
Hua, Tian
Hanqing, Chen
Li, Chen
Binyan, Yu
Jing, Ma
author_facet Xiping, Zhang
Hua, Tian
Hanqing, Chen
Li, Chen
Binyan, Yu
Jing, Ma
author_sort Xiping, Zhang
collection PubMed
description BACKGROUND: To investigate the effects of Baicalin and Octreotide on inflammatory mediators and pancreatic acinar cells apoptosis of rats with severe acute pancreatitis (SAP). METHODS: SD rats were randomly divided into sham operated group (I group), model control group (II group), Baicalin treated group (III group) and Octreotide treated group (IV group). Each group was also divided into subgroup of 3, 6 and 12 h (n = 15). The mortality rate, ascites/body weight ratio as well as the level of endotoxin, NO and ET-1 in blood were measured. The pathological severity score of pancreas, apoptotic indexes, and expression levels of Bax and Bcl-2 proteins in each group were investigated. RESULTS: The survival rate of III and IV group has a significant difference compared with II group (P(12 h) < 0.05). The ascites volume, contents of inflammatory mediators in blood and pathological severity score of pancreas of III and IV group declined at different degrees compared to II group (P < 0.05, P < 0.01 or P < 0.001). Apoptotic index in III group was significantly higher than that in II group at 3 and 6 h (P(3, 6 h) < 0.05). Apoptotic index in IV group was significantly higher than that in II group at pancreatic tail at 6 h (P(6 h) < 0.05). Expression level of Bax in III group was significantly higher than that in II group (pancreatic head P(3 h,6 h) < 0.01, pancreatic tail P(3 h) < 0.001). CONCLUSIONS: Compared with Octreotide in the treatment of SAP, the protective mechanisms of Baicalin include reducing the excessive inflammatory mediators’ release, inducing the pancreatic acinar cells apoptosis.
format Online
Article
Text
id pubmed-3129065
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher Medknow Publications Pvt Ltd
record_format MEDLINE/PubMed
spelling pubmed-31290652011-07-19 Effects of Baicalin on inflammatory mediators and pancreatic acinar cell apoptosis in rats with sever acute pancreatitis Xiping, Zhang Hua, Tian Hanqing, Chen Li, Chen Binyan, Yu Jing, Ma J Res Med Sci Original Article BACKGROUND: To investigate the effects of Baicalin and Octreotide on inflammatory mediators and pancreatic acinar cells apoptosis of rats with severe acute pancreatitis (SAP). METHODS: SD rats were randomly divided into sham operated group (I group), model control group (II group), Baicalin treated group (III group) and Octreotide treated group (IV group). Each group was also divided into subgroup of 3, 6 and 12 h (n = 15). The mortality rate, ascites/body weight ratio as well as the level of endotoxin, NO and ET-1 in blood were measured. The pathological severity score of pancreas, apoptotic indexes, and expression levels of Bax and Bcl-2 proteins in each group were investigated. RESULTS: The survival rate of III and IV group has a significant difference compared with II group (P(12 h) < 0.05). The ascites volume, contents of inflammatory mediators in blood and pathological severity score of pancreas of III and IV group declined at different degrees compared to II group (P < 0.05, P < 0.01 or P < 0.001). Apoptotic index in III group was significantly higher than that in II group at 3 and 6 h (P(3, 6 h) < 0.05). Apoptotic index in IV group was significantly higher than that in II group at pancreatic tail at 6 h (P(6 h) < 0.05). Expression level of Bax in III group was significantly higher than that in II group (pancreatic head P(3 h,6 h) < 0.01, pancreatic tail P(3 h) < 0.001). CONCLUSIONS: Compared with Octreotide in the treatment of SAP, the protective mechanisms of Baicalin include reducing the excessive inflammatory mediators’ release, inducing the pancreatic acinar cells apoptosis. Medknow Publications Pvt Ltd 2009 /pmc/articles/PMC3129065/ /pubmed/21772857 Text en © Journal of Research in Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Xiping, Zhang
Hua, Tian
Hanqing, Chen
Li, Chen
Binyan, Yu
Jing, Ma
Effects of Baicalin on inflammatory mediators and pancreatic acinar cell apoptosis in rats with sever acute pancreatitis
title Effects of Baicalin on inflammatory mediators and pancreatic acinar cell apoptosis in rats with sever acute pancreatitis
title_full Effects of Baicalin on inflammatory mediators and pancreatic acinar cell apoptosis in rats with sever acute pancreatitis
title_fullStr Effects of Baicalin on inflammatory mediators and pancreatic acinar cell apoptosis in rats with sever acute pancreatitis
title_full_unstemmed Effects of Baicalin on inflammatory mediators and pancreatic acinar cell apoptosis in rats with sever acute pancreatitis
title_short Effects of Baicalin on inflammatory mediators and pancreatic acinar cell apoptosis in rats with sever acute pancreatitis
title_sort effects of baicalin on inflammatory mediators and pancreatic acinar cell apoptosis in rats with sever acute pancreatitis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3129065/
https://www.ncbi.nlm.nih.gov/pubmed/21772857
work_keys_str_mv AT xipingzhang effectsofbaicalinoninflammatorymediatorsandpancreaticacinarcellapoptosisinratswithseveracutepancreatitis
AT huatian effectsofbaicalinoninflammatorymediatorsandpancreaticacinarcellapoptosisinratswithseveracutepancreatitis
AT hanqingchen effectsofbaicalinoninflammatorymediatorsandpancreaticacinarcellapoptosisinratswithseveracutepancreatitis
AT lichen effectsofbaicalinoninflammatorymediatorsandpancreaticacinarcellapoptosisinratswithseveracutepancreatitis
AT binyanyu effectsofbaicalinoninflammatorymediatorsandpancreaticacinarcellapoptosisinratswithseveracutepancreatitis
AT jingma effectsofbaicalinoninflammatorymediatorsandpancreaticacinarcellapoptosisinratswithseveracutepancreatitis