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Uniform Distribution of Elongating RNA Polymerase II Complexes in Transcribed Gene Locus
The intensity of gene transcription is generally reflected by the level of RNA polymerase II (RNAPII) recruitment to the gene. However, genome-wide studies of polymerase occupancy indicate that RNAPII distribution varies among genes. In some loci more polymerases are found in the 5′ region, whereas...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Biochemistry and Molecular Biology
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3129163/ https://www.ncbi.nlm.nih.gov/pubmed/21606489 http://dx.doi.org/10.1074/jbc.M111.230805 |
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author | Peil, Kadri Värv, Signe Lõoke, Marko Kristjuhan, Kersti Kristjuhan, Arnold |
author_facet | Peil, Kadri Värv, Signe Lõoke, Marko Kristjuhan, Kersti Kristjuhan, Arnold |
author_sort | Peil, Kadri |
collection | PubMed |
description | The intensity of gene transcription is generally reflected by the level of RNA polymerase II (RNAPII) recruitment to the gene. However, genome-wide studies of polymerase occupancy indicate that RNAPII distribution varies among genes. In some loci more polymerases are found in the 5′ region, whereas in other loci, in the 3′ region of the gene. We studied the distribution of elongating RNAPII complexes at highly transcribed GAL-VPS13 locus in Saccharomyces cerevisiae and found that in the cell population the amount of polymerases gradually decreased toward the 3′ end of the gene. However, the conventional chromatin immunoprecipitation assay averages the signal from the cell population, and no data on single cell level can be gathered. To study the spacing of elongating polymerases on single chromosomes, we used a sequential chromatin immunoprecipitation assay for the detection of multiple RNAPII complexes on the same DNA fragment. Our results demonstrate uniform distribution of elongating polymerases throughout all regions of the GAL-VPS13 gene. |
format | Online Article Text |
id | pubmed-3129163 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | American Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-31291632011-07-08 Uniform Distribution of Elongating RNA Polymerase II Complexes in Transcribed Gene Locus Peil, Kadri Värv, Signe Lõoke, Marko Kristjuhan, Kersti Kristjuhan, Arnold J Biol Chem Gene Regulation The intensity of gene transcription is generally reflected by the level of RNA polymerase II (RNAPII) recruitment to the gene. However, genome-wide studies of polymerase occupancy indicate that RNAPII distribution varies among genes. In some loci more polymerases are found in the 5′ region, whereas in other loci, in the 3′ region of the gene. We studied the distribution of elongating RNAPII complexes at highly transcribed GAL-VPS13 locus in Saccharomyces cerevisiae and found that in the cell population the amount of polymerases gradually decreased toward the 3′ end of the gene. However, the conventional chromatin immunoprecipitation assay averages the signal from the cell population, and no data on single cell level can be gathered. To study the spacing of elongating polymerases on single chromosomes, we used a sequential chromatin immunoprecipitation assay for the detection of multiple RNAPII complexes on the same DNA fragment. Our results demonstrate uniform distribution of elongating polymerases throughout all regions of the GAL-VPS13 gene. American Society for Biochemistry and Molecular Biology 2011-07-08 2011-05-23 /pmc/articles/PMC3129163/ /pubmed/21606489 http://dx.doi.org/10.1074/jbc.M111.230805 Text en © 2011 by The American Society for Biochemistry and Molecular Biology, Inc. Author's Choice—Final version full access. Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) applies to Author Choice Articles |
spellingShingle | Gene Regulation Peil, Kadri Värv, Signe Lõoke, Marko Kristjuhan, Kersti Kristjuhan, Arnold Uniform Distribution of Elongating RNA Polymerase II Complexes in Transcribed Gene Locus |
title | Uniform Distribution of Elongating RNA Polymerase II Complexes in Transcribed Gene Locus |
title_full | Uniform Distribution of Elongating RNA Polymerase II Complexes in Transcribed Gene Locus |
title_fullStr | Uniform Distribution of Elongating RNA Polymerase II Complexes in Transcribed Gene Locus |
title_full_unstemmed | Uniform Distribution of Elongating RNA Polymerase II Complexes in Transcribed Gene Locus |
title_short | Uniform Distribution of Elongating RNA Polymerase II Complexes in Transcribed Gene Locus |
title_sort | uniform distribution of elongating rna polymerase ii complexes in transcribed gene locus |
topic | Gene Regulation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3129163/ https://www.ncbi.nlm.nih.gov/pubmed/21606489 http://dx.doi.org/10.1074/jbc.M111.230805 |
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