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Improving RNA-Seq expression estimates by correcting for fragment bias

The biochemistry of RNA-Seq library preparation results in cDNA fragments that are not uniformly distributed within the transcripts they represent. This non-uniformity must be accounted for when estimating expression levels, and we show how to perform the needed corrections using a likelihood based...

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Detalles Bibliográficos
Autores principales:	Roberts, Adam, Trapnell, Cole, Donaghey, Julie, Rinn, John L, Pachter, Lior
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2011
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3129672/ https://www.ncbi.nlm.nih.gov/pubmed/21410973 http://dx.doi.org/10.1186/gb-2011-12-3-r22

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