A vertebrate case study of the quality of assemblies derived from next-generation sequences

The unparalleled efficiency of next-generation sequencing (NGS) has prompted widespread adoption, but significant problems remain in the use of NGS data for whole genome assembly. We explore the advantages and disadvantages of chicken genome assemblies generated using a variety of sequencing and ass...

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Detalles Bibliográficos
Autores principales: Ye, Liang, Hillier, LaDeana W, Minx, Patrick, Thane, Nay, Locke, Devin P, Martin, John C, Chen, Lei, Mitreva, Makedonka, Miller, Jason R, Haub, Kevin V, Dooling, David J, Mardis, Elaine R, Wilson, Richard K, Weinstock, George M, Warren, Wesley C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3129681/
https://www.ncbi.nlm.nih.gov/pubmed/21453517
http://dx.doi.org/10.1186/gb-2011-12-3-r31
Descripción
Sumario:The unparalleled efficiency of next-generation sequencing (NGS) has prompted widespread adoption, but significant problems remain in the use of NGS data for whole genome assembly. We explore the advantages and disadvantages of chicken genome assemblies generated using a variety of sequencing and assembly methodologies. NGS assemblies are equivalent in some ways to a Sanger-based assembly yet deficient in others. Nonetheless, these assemblies are sufficient for the identification of the majority of genes and can reveal novel sequences when compared to existing assembly references.

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