Pipeline for Large-Scale Microdroplet Bisulfite PCR-Based Sequencing Allows the Tracking of Hepitype Evolution in Tumors

Cytosine methylation provides an epigenetic level of cellular plasticity that is important for development, differentiation and cancerogenesis. We adopted microdroplet PCR to bisulfite treated target DNA in combination with second generation sequencing to simultaneously assess DNA sequence and methy...

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Detalles Bibliográficos
Autores principales: Herrmann, Alexander, Haake, Andrea, Ammerpohl, Ole, Martin-Guerrero, Idoia, Szafranski, Karol, Stemshorn, Kathryn, Nothnagel, Michael, Kotsopoulos, Steve K., Richter, Julia, Warner, Jason, Olson, Jeff, Link, Darren R., Schreiber, Stefan, Krawczak, Michael, Platzer, Matthias, Nürnberg, Peter, Siebert, Reiner, Hampe, Jochen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3130030/
https://www.ncbi.nlm.nih.gov/pubmed/21750708
http://dx.doi.org/10.1371/journal.pone.0021332

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