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Expression of aspartyl protease and C3HC4-type RING zinc finger genes are responsive to ascorbic acid in Arabidopsis thaliana

Ascorbate (AsA) is a redox buffer and enzyme cofactor with various proposed functions in stress responses and growth. The aim was to identify genes whose transcript levels respond to changes in leaf AsA. The AsA-deficient Arabidopsis mutant vtc2-1 was incubated with the AsA precursor L-galactono-1,4...

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Autores principales: Gao, Yongshun, Nishikawa, Hitoshi, Badejo, Adebanjo Ayobamidele, Shibata, Hitoshi, Sawa, Yoshihiro, Nakagawa, Tsuyoshi, Maruta, Takanori, Shigeoka, Shigeru, Smirnoff, Nicholas, Ishikawa, Takahiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3130181/
https://www.ncbi.nlm.nih.gov/pubmed/21421703
http://dx.doi.org/10.1093/jxb/err068
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author Gao, Yongshun
Nishikawa, Hitoshi
Badejo, Adebanjo Ayobamidele
Shibata, Hitoshi
Sawa, Yoshihiro
Nakagawa, Tsuyoshi
Maruta, Takanori
Shigeoka, Shigeru
Smirnoff, Nicholas
Ishikawa, Takahiro
author_facet Gao, Yongshun
Nishikawa, Hitoshi
Badejo, Adebanjo Ayobamidele
Shibata, Hitoshi
Sawa, Yoshihiro
Nakagawa, Tsuyoshi
Maruta, Takanori
Shigeoka, Shigeru
Smirnoff, Nicholas
Ishikawa, Takahiro
author_sort Gao, Yongshun
collection PubMed
description Ascorbate (AsA) is a redox buffer and enzyme cofactor with various proposed functions in stress responses and growth. The aim was to identify genes whose transcript levels respond to changes in leaf AsA. The AsA-deficient Arabidopsis mutant vtc2-1 was incubated with the AsA precursor L-galactono-1,4-lactone (L-GalL) to increase leaf AsA concentration. Differentially expressed genes screened by DNA microarray were further characterized for AsA responsiveness in wild-type plants. The analysis of 14 candidates by real-time PCR identified an aspartyl protease gene (ASP, At1g66180) and a C3HC4-type RING zinc finger gene (AtATL15, At1g22500) whose transcripts were rapidly responsive to increases in AsA pool size caused by L-GalL and AsA supplementation and light. Transgenic Arabidopsis plants expressing an AtATL15 promoter::luciferase reporter confirmed that the promoter is L-GalL, AsA, and light responsive. The expression patterns of ASP and AtATL15 suggest they have roles in growth regulation. The promoter of AtATL15 is responsive to AsA status and will provide a tool to investigate the functions of AsA in plants further.
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spelling pubmed-31301812011-07-06 Expression of aspartyl protease and C3HC4-type RING zinc finger genes are responsive to ascorbic acid in Arabidopsis thaliana Gao, Yongshun Nishikawa, Hitoshi Badejo, Adebanjo Ayobamidele Shibata, Hitoshi Sawa, Yoshihiro Nakagawa, Tsuyoshi Maruta, Takanori Shigeoka, Shigeru Smirnoff, Nicholas Ishikawa, Takahiro J Exp Bot Research Papers Ascorbate (AsA) is a redox buffer and enzyme cofactor with various proposed functions in stress responses and growth. The aim was to identify genes whose transcript levels respond to changes in leaf AsA. The AsA-deficient Arabidopsis mutant vtc2-1 was incubated with the AsA precursor L-galactono-1,4-lactone (L-GalL) to increase leaf AsA concentration. Differentially expressed genes screened by DNA microarray were further characterized for AsA responsiveness in wild-type plants. The analysis of 14 candidates by real-time PCR identified an aspartyl protease gene (ASP, At1g66180) and a C3HC4-type RING zinc finger gene (AtATL15, At1g22500) whose transcripts were rapidly responsive to increases in AsA pool size caused by L-GalL and AsA supplementation and light. Transgenic Arabidopsis plants expressing an AtATL15 promoter::luciferase reporter confirmed that the promoter is L-GalL, AsA, and light responsive. The expression patterns of ASP and AtATL15 suggest they have roles in growth regulation. The promoter of AtATL15 is responsive to AsA status and will provide a tool to investigate the functions of AsA in plants further. Oxford University Press 2011-06 2011-03-18 /pmc/articles/PMC3130181/ /pubmed/21421703 http://dx.doi.org/10.1093/jxb/err068 Text en © 2011 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)
spellingShingle Research Papers
Gao, Yongshun
Nishikawa, Hitoshi
Badejo, Adebanjo Ayobamidele
Shibata, Hitoshi
Sawa, Yoshihiro
Nakagawa, Tsuyoshi
Maruta, Takanori
Shigeoka, Shigeru
Smirnoff, Nicholas
Ishikawa, Takahiro
Expression of aspartyl protease and C3HC4-type RING zinc finger genes are responsive to ascorbic acid in Arabidopsis thaliana
title Expression of aspartyl protease and C3HC4-type RING zinc finger genes are responsive to ascorbic acid in Arabidopsis thaliana
title_full Expression of aspartyl protease and C3HC4-type RING zinc finger genes are responsive to ascorbic acid in Arabidopsis thaliana
title_fullStr Expression of aspartyl protease and C3HC4-type RING zinc finger genes are responsive to ascorbic acid in Arabidopsis thaliana
title_full_unstemmed Expression of aspartyl protease and C3HC4-type RING zinc finger genes are responsive to ascorbic acid in Arabidopsis thaliana
title_short Expression of aspartyl protease and C3HC4-type RING zinc finger genes are responsive to ascorbic acid in Arabidopsis thaliana
title_sort expression of aspartyl protease and c3hc4-type ring zinc finger genes are responsive to ascorbic acid in arabidopsis thaliana
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3130181/
https://www.ncbi.nlm.nih.gov/pubmed/21421703
http://dx.doi.org/10.1093/jxb/err068
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