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A method for counting PCR template molecules with application to next-generation sequencing
Amplification by polymerase chain reaction is often used in the preparation of template DNA molecules for next-generation sequencing. Amplification increases the number of available molecules for sequencing but changes the representation of the template molecules in the amplified product and introdu...
Autores principales: | Casbon, James A., Osborne, Robert J., Brenner, Sydney, Lichtenstein, Conrad P. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3130290/ https://www.ncbi.nlm.nih.gov/pubmed/21490082 http://dx.doi.org/10.1093/nar/gkr217 |
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