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Multiplex single-nucleotide polymorphism typing of the human Y chromosome using TaqMan probes

BACKGROUND: The analysis of human Y-chromosome variation in the context of population genetics and forensics requires the genotyping of dozens to hundreds of selected single-nucleotide polymorphisms (SNPs). In the present study, we developed a 121-plex (121 SNPs in a single array) TaqMan array capab...

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Autores principales: Martínez-Cruz, Begoña, Ziegle, Janet, Sanz, Paula, Sotelo, Graciela, Anglada, Roger, Plaza, Stéphanie, Comas, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3130649/
https://www.ncbi.nlm.nih.gov/pubmed/21627798
http://dx.doi.org/10.1186/2041-2223-2-13
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author Martínez-Cruz, Begoña
Ziegle, Janet
Sanz, Paula
Sotelo, Graciela
Anglada, Roger
Plaza, Stéphanie
Comas, David
author_facet Martínez-Cruz, Begoña
Ziegle, Janet
Sanz, Paula
Sotelo, Graciela
Anglada, Roger
Plaza, Stéphanie
Comas, David
author_sort Martínez-Cruz, Begoña
collection PubMed
description BACKGROUND: The analysis of human Y-chromosome variation in the context of population genetics and forensics requires the genotyping of dozens to hundreds of selected single-nucleotide polymorphisms (SNPs). In the present study, we developed a 121-plex (121 SNPs in a single array) TaqMan array capable of distinguishing most haplogroups and subhaplogroups on the Y-chromosome human phylogeny in Europe. RESULTS: We present data from 264 samples from several European areas and ethnic groups. The array developed in this study shows >99% accuracy of assignation to the Y human phylogeny (with an average call rate of genotypes >96%). CONCLUSIONS: We have created and evaluated a robust and accurate Y-chromosome multiplex which minimises the possible errors due to mixup when typing the same sample in several independent reactions.
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spelling pubmed-31306492011-07-07 Multiplex single-nucleotide polymorphism typing of the human Y chromosome using TaqMan probes Martínez-Cruz, Begoña Ziegle, Janet Sanz, Paula Sotelo, Graciela Anglada, Roger Plaza, Stéphanie Comas, David Investig Genet Research BACKGROUND: The analysis of human Y-chromosome variation in the context of population genetics and forensics requires the genotyping of dozens to hundreds of selected single-nucleotide polymorphisms (SNPs). In the present study, we developed a 121-plex (121 SNPs in a single array) TaqMan array capable of distinguishing most haplogroups and subhaplogroups on the Y-chromosome human phylogeny in Europe. RESULTS: We present data from 264 samples from several European areas and ethnic groups. The array developed in this study shows >99% accuracy of assignation to the Y human phylogeny (with an average call rate of genotypes >96%). CONCLUSIONS: We have created and evaluated a robust and accurate Y-chromosome multiplex which minimises the possible errors due to mixup when typing the same sample in several independent reactions. BioMed Central 2011-05-31 /pmc/articles/PMC3130649/ /pubmed/21627798 http://dx.doi.org/10.1186/2041-2223-2-13 Text en Copyright ©2011 Martínez-Cruz et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Martínez-Cruz, Begoña
Ziegle, Janet
Sanz, Paula
Sotelo, Graciela
Anglada, Roger
Plaza, Stéphanie
Comas, David
Multiplex single-nucleotide polymorphism typing of the human Y chromosome using TaqMan probes
title Multiplex single-nucleotide polymorphism typing of the human Y chromosome using TaqMan probes
title_full Multiplex single-nucleotide polymorphism typing of the human Y chromosome using TaqMan probes
title_fullStr Multiplex single-nucleotide polymorphism typing of the human Y chromosome using TaqMan probes
title_full_unstemmed Multiplex single-nucleotide polymorphism typing of the human Y chromosome using TaqMan probes
title_short Multiplex single-nucleotide polymorphism typing of the human Y chromosome using TaqMan probes
title_sort multiplex single-nucleotide polymorphism typing of the human y chromosome using taqman probes
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3130649/
https://www.ncbi.nlm.nih.gov/pubmed/21627798
http://dx.doi.org/10.1186/2041-2223-2-13
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