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GoldenBraid: An Iterative Cloning System for Standardized Assembly of Reusable Genetic Modules
Synthetic Biology requires efficient and versatile DNA assembly systems to facilitate the building of new genetic modules/pathways from basic DNA parts in a standardized way. Here we present GoldenBraid (GB), a standardized assembly system based on type IIS restriction enzymes that allows the indefi...
Autores principales: | Sarrion-Perdigones, Alejandro, Falconi, Erica Elvira, Zandalinas, Sara I., Juárez, Paloma, Fernández-del-Carmen, Asun, Granell, Antonio, Orzaez, Diego |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3131274/ https://www.ncbi.nlm.nih.gov/pubmed/21750718 http://dx.doi.org/10.1371/journal.pone.0021622 |
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