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Isolation, Characterization and Biological Evaluation of Jellyfish Collagen for Use in Biomedical Applications
Fibrillar collagens are the more abundant extracellular proteins. They form a metazoan-specific family, and are highly conserved from sponge to human. Their structural and physiological properties have been successfully used in the food, cosmetic, and pharmaceutical industries. On the other hand, th...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3131555/ https://www.ncbi.nlm.nih.gov/pubmed/21747742 http://dx.doi.org/10.3390/md9060967 |
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author | Addad, Sourour Exposito, Jean-Yves Faye, Clément Ricard-Blum, Sylvie Lethias, Claire |
author_facet | Addad, Sourour Exposito, Jean-Yves Faye, Clément Ricard-Blum, Sylvie Lethias, Claire |
author_sort | Addad, Sourour |
collection | PubMed |
description | Fibrillar collagens are the more abundant extracellular proteins. They form a metazoan-specific family, and are highly conserved from sponge to human. Their structural and physiological properties have been successfully used in the food, cosmetic, and pharmaceutical industries. On the other hand, the increase of jellyfish has led us to consider this marine animal as a natural product for food and medicine. Here, we have tested different Mediterranean jellyfish species in order to investigate the economic potential of their collagens. We have studied different methods of collagen purification (tissues and experimental procedures). The best collagen yield was obtained using Rhizostoma pulmo oral arms and the pepsin extraction method (2–10 mg collagen/g of wet tissue). Although a significant yield was obtained with Cotylorhiza tuberculata (0.45 mg/g), R. pulmo was used for further experiments, this jellyfish being considered as harmless to humans and being an abundant source of material. Then, we compared the biological properties of R. pulmo collagen with mammalian fibrillar collagens in cell cytotoxicity assays and cell adhesion. There was no statistical difference in cytotoxicity (p > 0.05) between R. pulmo collagen and rat type I collagen. However, since heparin inhibits cell adhesion to jellyfish-native collagen by 55%, the main difference is that heparan sulfate proteoglycans could be preferentially involved in fibroblast and osteoblast adhesion to jellyfish collagens. Our data confirm the broad harmlessness of jellyfish collagens, and their biological effect on human cells that are similar to that of mammalian type I collagen. Given the bioavailability of jellyfish collagen and its biological properties, this marine material is thus a good candidate for replacing bovine or human collagens in selected biomedical applications. |
format | Online Article Text |
id | pubmed-3131555 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Molecular Diversity Preservation International |
record_format | MEDLINE/PubMed |
spelling | pubmed-31315552011-07-11 Isolation, Characterization and Biological Evaluation of Jellyfish Collagen for Use in Biomedical Applications Addad, Sourour Exposito, Jean-Yves Faye, Clément Ricard-Blum, Sylvie Lethias, Claire Mar Drugs Article Fibrillar collagens are the more abundant extracellular proteins. They form a metazoan-specific family, and are highly conserved from sponge to human. Their structural and physiological properties have been successfully used in the food, cosmetic, and pharmaceutical industries. On the other hand, the increase of jellyfish has led us to consider this marine animal as a natural product for food and medicine. Here, we have tested different Mediterranean jellyfish species in order to investigate the economic potential of their collagens. We have studied different methods of collagen purification (tissues and experimental procedures). The best collagen yield was obtained using Rhizostoma pulmo oral arms and the pepsin extraction method (2–10 mg collagen/g of wet tissue). Although a significant yield was obtained with Cotylorhiza tuberculata (0.45 mg/g), R. pulmo was used for further experiments, this jellyfish being considered as harmless to humans and being an abundant source of material. Then, we compared the biological properties of R. pulmo collagen with mammalian fibrillar collagens in cell cytotoxicity assays and cell adhesion. There was no statistical difference in cytotoxicity (p > 0.05) between R. pulmo collagen and rat type I collagen. However, since heparin inhibits cell adhesion to jellyfish-native collagen by 55%, the main difference is that heparan sulfate proteoglycans could be preferentially involved in fibroblast and osteoblast adhesion to jellyfish collagens. Our data confirm the broad harmlessness of jellyfish collagens, and their biological effect on human cells that are similar to that of mammalian type I collagen. Given the bioavailability of jellyfish collagen and its biological properties, this marine material is thus a good candidate for replacing bovine or human collagens in selected biomedical applications. Molecular Diversity Preservation International 2011-06-07 /pmc/articles/PMC3131555/ /pubmed/21747742 http://dx.doi.org/10.3390/md9060967 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Addad, Sourour Exposito, Jean-Yves Faye, Clément Ricard-Blum, Sylvie Lethias, Claire Isolation, Characterization and Biological Evaluation of Jellyfish Collagen for Use in Biomedical Applications |
title | Isolation, Characterization and Biological Evaluation of Jellyfish Collagen for Use in Biomedical Applications |
title_full | Isolation, Characterization and Biological Evaluation of Jellyfish Collagen for Use in Biomedical Applications |
title_fullStr | Isolation, Characterization and Biological Evaluation of Jellyfish Collagen for Use in Biomedical Applications |
title_full_unstemmed | Isolation, Characterization and Biological Evaluation of Jellyfish Collagen for Use in Biomedical Applications |
title_short | Isolation, Characterization and Biological Evaluation of Jellyfish Collagen for Use in Biomedical Applications |
title_sort | isolation, characterization and biological evaluation of jellyfish collagen for use in biomedical applications |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3131555/ https://www.ncbi.nlm.nih.gov/pubmed/21747742 http://dx.doi.org/10.3390/md9060967 |
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