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Resistance to Elsinoë Ampelina and Expression of Related Resistant Genes in Vitis Rotundifolia Michx. Grapes

Muscadine grapes (Vitis rotundifolia Michx) are considered as excellent genetic resources for grape breeding programs as they are known for their hardiness and resistance to pests and diseases. However, contrary to popular belief, our study indicated that not all muscadine cultivars are resistant to...

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Autores principales: Louime, Clifford, Lu, Jiang, Onokpise, Oghenekome, Vasanthaiah, Hemanth K. N., Kambiranda, Devaiah, Basha, Sheikh M., Yun, Hae Keun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3131573/
https://www.ncbi.nlm.nih.gov/pubmed/21747689
http://dx.doi.org/10.3390/ijms12063473
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author Louime, Clifford
Lu, Jiang
Onokpise, Oghenekome
Vasanthaiah, Hemanth K. N.
Kambiranda, Devaiah
Basha, Sheikh M.
Yun, Hae Keun
author_facet Louime, Clifford
Lu, Jiang
Onokpise, Oghenekome
Vasanthaiah, Hemanth K. N.
Kambiranda, Devaiah
Basha, Sheikh M.
Yun, Hae Keun
author_sort Louime, Clifford
collection PubMed
description Muscadine grapes (Vitis rotundifolia Michx) are considered as excellent genetic resources for grape breeding programs as they are known for their hardiness and resistance to pests and diseases. However, contrary to popular belief, our study indicated that not all muscadine cultivars are resistant to anthracnose disease. In order to identify a source of genetic tolerance towards anthracnose among muscadine cultivars, a series of in-situ and ex-situ experiments were conducted through strict and sensitive screening processes. Two consecutive years of field evaluation of 54 grape cultivars showed various levels of anthracnose incidence among the cultivars between a scale of 0 (tolerant) to 5 (highly-susceptible). Resistance bioassay by inoculation of different spore densities of Elsinoë ampelina on 40 cultivars presented similar results and was consistent with those obtained from the field test. A real-time PCR analysis was conducted to investigate differences of gene expression between susceptible and tolerant cultivars and to confirm results by phenotypic identification. Expression of genes encoding chalcone synthase, stilbene synthase, polygalacturonase-inhibiting protein, chitinase and lipid transfer-protein was only detected in tolerant cultivars. Resistant muscadine cultivars identified in this study could be excellent candidates for grape disease resistance breeding programs.
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spelling pubmed-31315732011-07-11 Resistance to Elsinoë Ampelina and Expression of Related Resistant Genes in Vitis Rotundifolia Michx. Grapes Louime, Clifford Lu, Jiang Onokpise, Oghenekome Vasanthaiah, Hemanth K. N. Kambiranda, Devaiah Basha, Sheikh M. Yun, Hae Keun Int J Mol Sci Article Muscadine grapes (Vitis rotundifolia Michx) are considered as excellent genetic resources for grape breeding programs as they are known for their hardiness and resistance to pests and diseases. However, contrary to popular belief, our study indicated that not all muscadine cultivars are resistant to anthracnose disease. In order to identify a source of genetic tolerance towards anthracnose among muscadine cultivars, a series of in-situ and ex-situ experiments were conducted through strict and sensitive screening processes. Two consecutive years of field evaluation of 54 grape cultivars showed various levels of anthracnose incidence among the cultivars between a scale of 0 (tolerant) to 5 (highly-susceptible). Resistance bioassay by inoculation of different spore densities of Elsinoë ampelina on 40 cultivars presented similar results and was consistent with those obtained from the field test. A real-time PCR analysis was conducted to investigate differences of gene expression between susceptible and tolerant cultivars and to confirm results by phenotypic identification. Expression of genes encoding chalcone synthase, stilbene synthase, polygalacturonase-inhibiting protein, chitinase and lipid transfer-protein was only detected in tolerant cultivars. Resistant muscadine cultivars identified in this study could be excellent candidates for grape disease resistance breeding programs. Molecular Diversity Preservation International (MDPI) 2011-06-01 /pmc/articles/PMC3131573/ /pubmed/21747689 http://dx.doi.org/10.3390/ijms12063473 Text en © 2011 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Louime, Clifford
Lu, Jiang
Onokpise, Oghenekome
Vasanthaiah, Hemanth K. N.
Kambiranda, Devaiah
Basha, Sheikh M.
Yun, Hae Keun
Resistance to Elsinoë Ampelina and Expression of Related Resistant Genes in Vitis Rotundifolia Michx. Grapes
title Resistance to Elsinoë Ampelina and Expression of Related Resistant Genes in Vitis Rotundifolia Michx. Grapes
title_full Resistance to Elsinoë Ampelina and Expression of Related Resistant Genes in Vitis Rotundifolia Michx. Grapes
title_fullStr Resistance to Elsinoë Ampelina and Expression of Related Resistant Genes in Vitis Rotundifolia Michx. Grapes
title_full_unstemmed Resistance to Elsinoë Ampelina and Expression of Related Resistant Genes in Vitis Rotundifolia Michx. Grapes
title_short Resistance to Elsinoë Ampelina and Expression of Related Resistant Genes in Vitis Rotundifolia Michx. Grapes
title_sort resistance to elsinoë ampelina and expression of related resistant genes in vitis rotundifolia michx. grapes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3131573/
https://www.ncbi.nlm.nih.gov/pubmed/21747689
http://dx.doi.org/10.3390/ijms12063473
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