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Differences in Soil Properties and Bacterial Communities between the Rhizosphere and Bulk Soil and among Different Production Areas of the Medicinal Plant Fritillaria thunbergii
To explore rhizosphere effects, geographical differences and their effects on the bacterial community associated with the geoherb Fritillaria thunbergii, some physicochemical properties of soil samples (3 sampling sites × 2 habitats (rhizosphere and bulk soil)) were measured and the soil bacterial c...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3131589/ https://www.ncbi.nlm.nih.gov/pubmed/21747705 http://dx.doi.org/10.3390/ijms12063770 |
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author | Shi, Ji-Yan Yuan, Xiao-Feng Lin, Hui-Rong Yang, Yuan-Qiang Li, Zong-Yuan |
author_facet | Shi, Ji-Yan Yuan, Xiao-Feng Lin, Hui-Rong Yang, Yuan-Qiang Li, Zong-Yuan |
author_sort | Shi, Ji-Yan |
collection | PubMed |
description | To explore rhizosphere effects, geographical differences and their effects on the bacterial community associated with the geoherb Fritillaria thunbergii, some physicochemical properties of soil samples (3 sampling sites × 2 habitats (rhizosphere and bulk soil)) were measured and the soil bacterial community detected by PCR-denaturing gradient gel electrophoresis (DGGE). Among the three regions, soil pH varied between 4.48 and 7.73 indicating that F. thunbergii could grow both in acid and slightly alkaline soil. As the authentic Dao-di producing area, Ningbo showed the highest soil quality with the highest content of organic matter (OM) (2.46%), phosphatase (268 mg kg(−1) 24 h(−1)) and urease activity (1481 mg kg(−1) 24 h(−1)). In comparison with the bulk soil, pH, organic carbon content, and phosphatase and urease activities were all lower in the rhizosphere, suggesting that the roots may secrete some unique metabolites in root exudates. Statistical analyses showed that soil properties of Ningbo and Panan in Zhejiang province were more similar to each other than those in Nantong in Jiangsu province. In addition, PCR-DGGE analysis showed that main bacterial population identified in F. thunbergii was proteobacteria (18 bands, 55%), acidobacteria (4, 12%), actinobacteria (4, 12%) and bacterioidetes (6, 18%). Overall, soil properties and microbial communities varied not only between the rhizosphere and bulk soil but also among the three regions. We suggest that the plant, together with the soil properties, cooperatively shape the structure of the rhizosphere bacteria, and that the soil properties have a close relationship with the geoherbalism of F. thunbergii. |
format | Online Article Text |
id | pubmed-3131589 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-31315892011-07-11 Differences in Soil Properties and Bacterial Communities between the Rhizosphere and Bulk Soil and among Different Production Areas of the Medicinal Plant Fritillaria thunbergii Shi, Ji-Yan Yuan, Xiao-Feng Lin, Hui-Rong Yang, Yuan-Qiang Li, Zong-Yuan Int J Mol Sci Article To explore rhizosphere effects, geographical differences and their effects on the bacterial community associated with the geoherb Fritillaria thunbergii, some physicochemical properties of soil samples (3 sampling sites × 2 habitats (rhizosphere and bulk soil)) were measured and the soil bacterial community detected by PCR-denaturing gradient gel electrophoresis (DGGE). Among the three regions, soil pH varied between 4.48 and 7.73 indicating that F. thunbergii could grow both in acid and slightly alkaline soil. As the authentic Dao-di producing area, Ningbo showed the highest soil quality with the highest content of organic matter (OM) (2.46%), phosphatase (268 mg kg(−1) 24 h(−1)) and urease activity (1481 mg kg(−1) 24 h(−1)). In comparison with the bulk soil, pH, organic carbon content, and phosphatase and urease activities were all lower in the rhizosphere, suggesting that the roots may secrete some unique metabolites in root exudates. Statistical analyses showed that soil properties of Ningbo and Panan in Zhejiang province were more similar to each other than those in Nantong in Jiangsu province. In addition, PCR-DGGE analysis showed that main bacterial population identified in F. thunbergii was proteobacteria (18 bands, 55%), acidobacteria (4, 12%), actinobacteria (4, 12%) and bacterioidetes (6, 18%). Overall, soil properties and microbial communities varied not only between the rhizosphere and bulk soil but also among the three regions. We suggest that the plant, together with the soil properties, cooperatively shape the structure of the rhizosphere bacteria, and that the soil properties have a close relationship with the geoherbalism of F. thunbergii. Molecular Diversity Preservation International (MDPI) 2011-06-09 /pmc/articles/PMC3131589/ /pubmed/21747705 http://dx.doi.org/10.3390/ijms12063770 Text en © 2011 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Shi, Ji-Yan Yuan, Xiao-Feng Lin, Hui-Rong Yang, Yuan-Qiang Li, Zong-Yuan Differences in Soil Properties and Bacterial Communities between the Rhizosphere and Bulk Soil and among Different Production Areas of the Medicinal Plant Fritillaria thunbergii |
title | Differences in Soil Properties and Bacterial Communities between the Rhizosphere and Bulk Soil and among Different Production Areas of the Medicinal Plant Fritillaria thunbergii |
title_full | Differences in Soil Properties and Bacterial Communities between the Rhizosphere and Bulk Soil and among Different Production Areas of the Medicinal Plant Fritillaria thunbergii |
title_fullStr | Differences in Soil Properties and Bacterial Communities between the Rhizosphere and Bulk Soil and among Different Production Areas of the Medicinal Plant Fritillaria thunbergii |
title_full_unstemmed | Differences in Soil Properties and Bacterial Communities between the Rhizosphere and Bulk Soil and among Different Production Areas of the Medicinal Plant Fritillaria thunbergii |
title_short | Differences in Soil Properties and Bacterial Communities between the Rhizosphere and Bulk Soil and among Different Production Areas of the Medicinal Plant Fritillaria thunbergii |
title_sort | differences in soil properties and bacterial communities between the rhizosphere and bulk soil and among different production areas of the medicinal plant fritillaria thunbergii |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3131589/ https://www.ncbi.nlm.nih.gov/pubmed/21747705 http://dx.doi.org/10.3390/ijms12063770 |
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