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Diet-induced metabolic hamster model of nonalcoholic fatty liver disease
BACKGROUND: Obesity, hypercholesterolemia, elevated triglycerides, and type 2 diabetes are major risk factors for metabolic syndrome. Hamsters, unlike rats or mice, respond well to diet-induced obesity, increase body mass and adiposity on group housing, and increase food intake due to social confron...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3131800/ https://www.ncbi.nlm.nih.gov/pubmed/21760736 http://dx.doi.org/10.2147/DMSO.S18435 |
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author | Bhathena, Jasmine Kulamarva, Arun Martoni, Christopher Urbanska, Aleksandra Malgorzata Malhotra, Meenakshi Paul, Arghya Prakash, Satya |
author_facet | Bhathena, Jasmine Kulamarva, Arun Martoni, Christopher Urbanska, Aleksandra Malgorzata Malhotra, Meenakshi Paul, Arghya Prakash, Satya |
author_sort | Bhathena, Jasmine |
collection | PubMed |
description | BACKGROUND: Obesity, hypercholesterolemia, elevated triglycerides, and type 2 diabetes are major risk factors for metabolic syndrome. Hamsters, unlike rats or mice, respond well to diet-induced obesity, increase body mass and adiposity on group housing, and increase food intake due to social confrontation-induced stress. They have a cardiovascular and hepatic system similar to that of humans, and can thus be a useful model for human pathophysiology. METHODS: Experiments were planned to develop a diet-induced Bio F(1)B Golden Syrian hamster model of dyslipidemia and associated nonalcoholic fatty liver disease in the metabolic syndrome. Hamsters were fed a normal control diet, a high-fat/high-cholesterol diet, a high-fat/high-cholesterol/methionine-deficient/choline-devoid diet, and a high-fat/high-cholesterol/choline-deficient diet. Serum total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, triglycerides, glucose, atherogenic index, and body weight were quantified biweekly. Fat deposition in the liver was observed and assessed following lipid staining with hematoxylin and eosin and with oil red O. RESULTS: In this study, we established a diet-induced Bio F(1)B Golden Syrian hamster model for studying dyslipidemia and associated nonalcoholic fatty liver disease in the metabolic syndrome. Hyperlipidemia and elevated serum glucose concentrations were induced using this diet. Atherogenic index was elevated, increasing the risk for a cardiovascular event. Histological analysis of liver specimens at the end of four weeks showed increased fat deposition in the liver of animals fed with a high-fat/high cholesterol diet, as compared to animals fed with the control diet. CONCLUSION: Our study established that hamsters fed with a high-fat/high-cholesterol diet developed fatty liver and mild diabetes. Bio F(1)B hamsters fed with a high-fat/high-cholesterol diet may thus be a good animal model for research on the treatment of diet-induced metabolic syndrome complicated by nonalcoholic fatty liver disease. |
format | Online Article Text |
id | pubmed-3131800 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-31318002011-07-14 Diet-induced metabolic hamster model of nonalcoholic fatty liver disease Bhathena, Jasmine Kulamarva, Arun Martoni, Christopher Urbanska, Aleksandra Malgorzata Malhotra, Meenakshi Paul, Arghya Prakash, Satya Diabetes Metab Syndr Obes Original Research BACKGROUND: Obesity, hypercholesterolemia, elevated triglycerides, and type 2 diabetes are major risk factors for metabolic syndrome. Hamsters, unlike rats or mice, respond well to diet-induced obesity, increase body mass and adiposity on group housing, and increase food intake due to social confrontation-induced stress. They have a cardiovascular and hepatic system similar to that of humans, and can thus be a useful model for human pathophysiology. METHODS: Experiments were planned to develop a diet-induced Bio F(1)B Golden Syrian hamster model of dyslipidemia and associated nonalcoholic fatty liver disease in the metabolic syndrome. Hamsters were fed a normal control diet, a high-fat/high-cholesterol diet, a high-fat/high-cholesterol/methionine-deficient/choline-devoid diet, and a high-fat/high-cholesterol/choline-deficient diet. Serum total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, triglycerides, glucose, atherogenic index, and body weight were quantified biweekly. Fat deposition in the liver was observed and assessed following lipid staining with hematoxylin and eosin and with oil red O. RESULTS: In this study, we established a diet-induced Bio F(1)B Golden Syrian hamster model for studying dyslipidemia and associated nonalcoholic fatty liver disease in the metabolic syndrome. Hyperlipidemia and elevated serum glucose concentrations were induced using this diet. Atherogenic index was elevated, increasing the risk for a cardiovascular event. Histological analysis of liver specimens at the end of four weeks showed increased fat deposition in the liver of animals fed with a high-fat/high cholesterol diet, as compared to animals fed with the control diet. CONCLUSION: Our study established that hamsters fed with a high-fat/high-cholesterol diet developed fatty liver and mild diabetes. Bio F(1)B hamsters fed with a high-fat/high-cholesterol diet may thus be a good animal model for research on the treatment of diet-induced metabolic syndrome complicated by nonalcoholic fatty liver disease. Dove Medical Press 2011-06-03 /pmc/articles/PMC3131800/ /pubmed/21760736 http://dx.doi.org/10.2147/DMSO.S18435 Text en © 2011 Bhathena et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. |
spellingShingle | Original Research Bhathena, Jasmine Kulamarva, Arun Martoni, Christopher Urbanska, Aleksandra Malgorzata Malhotra, Meenakshi Paul, Arghya Prakash, Satya Diet-induced metabolic hamster model of nonalcoholic fatty liver disease |
title | Diet-induced metabolic hamster model of nonalcoholic fatty liver disease |
title_full | Diet-induced metabolic hamster model of nonalcoholic fatty liver disease |
title_fullStr | Diet-induced metabolic hamster model of nonalcoholic fatty liver disease |
title_full_unstemmed | Diet-induced metabolic hamster model of nonalcoholic fatty liver disease |
title_short | Diet-induced metabolic hamster model of nonalcoholic fatty liver disease |
title_sort | diet-induced metabolic hamster model of nonalcoholic fatty liver disease |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3131800/ https://www.ncbi.nlm.nih.gov/pubmed/21760736 http://dx.doi.org/10.2147/DMSO.S18435 |
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