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GI-type T4SS-mediated horizontal transfer of the 89K pathogenicity island in epidemic Streptococcus suis serotype 2
Pathogenicity islands (PAIs), a distinct type of genomic island (GI), play important roles in the rapid adaptation and increased virulence of pathogens. 89K is a newly identified PAI in epidemic Streptococcus suis isolates that are related to the two recent large-scale outbreaks of human infection i...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3132442/ https://www.ncbi.nlm.nih.gov/pubmed/21244532 http://dx.doi.org/10.1111/j.1365-2958.2011.07553.x |
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author | Li, Ming Shen, Xiaodong Yan, Jinghua Han, Huiming Zheng, Beiwen Liu, Di Cheng, Hao Zhao, Yan Rao, Xiancai Wang, Changjun Tang, Jiaqi Hu, Fuquan Gao, George F |
author_facet | Li, Ming Shen, Xiaodong Yan, Jinghua Han, Huiming Zheng, Beiwen Liu, Di Cheng, Hao Zhao, Yan Rao, Xiancai Wang, Changjun Tang, Jiaqi Hu, Fuquan Gao, George F |
author_sort | Li, Ming |
collection | PubMed |
description | Pathogenicity islands (PAIs), a distinct type of genomic island (GI), play important roles in the rapid adaptation and increased virulence of pathogens. 89K is a newly identified PAI in epidemic Streptococcus suis isolates that are related to the two recent large-scale outbreaks of human infection in China. However, its mechanism of evolution and contribution to the epidemic spread of S. suis 2 remain unknown. In this study, the potential for mobilization of 89K was evaluated, and its putative transfer mechanism was investigated. We report that 89K can spontaneously excise to form an extrachromosomal circular product. The precise excision is mediated by an 89K-borne integrase through site-specific recombination, with help from an excisionase. The 89K excision intermediate acts as a substrate for lateral transfer to non-89K S. suis 2 recipients, where it reintegrates site-specifically into the target site. The conjugal transfer of 89K occurred via a GI type IV secretion system (T4SS) encoded in 89K, at a frequency of 10(−6) transconjugants per donor. This is the first demonstration of horizontal transfer of a Gram-positive PAI mediated by a GI-type T4SS. We propose that these genetic events are important in the emergence, pathogenesis and persistence of epidemic S. suis 2 strains. |
format | Online Article Text |
id | pubmed-3132442 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-31324422011-07-11 GI-type T4SS-mediated horizontal transfer of the 89K pathogenicity island in epidemic Streptococcus suis serotype 2 Li, Ming Shen, Xiaodong Yan, Jinghua Han, Huiming Zheng, Beiwen Liu, Di Cheng, Hao Zhao, Yan Rao, Xiancai Wang, Changjun Tang, Jiaqi Hu, Fuquan Gao, George F Mol Microbiol Research Articles Pathogenicity islands (PAIs), a distinct type of genomic island (GI), play important roles in the rapid adaptation and increased virulence of pathogens. 89K is a newly identified PAI in epidemic Streptococcus suis isolates that are related to the two recent large-scale outbreaks of human infection in China. However, its mechanism of evolution and contribution to the epidemic spread of S. suis 2 remain unknown. In this study, the potential for mobilization of 89K was evaluated, and its putative transfer mechanism was investigated. We report that 89K can spontaneously excise to form an extrachromosomal circular product. The precise excision is mediated by an 89K-borne integrase through site-specific recombination, with help from an excisionase. The 89K excision intermediate acts as a substrate for lateral transfer to non-89K S. suis 2 recipients, where it reintegrates site-specifically into the target site. The conjugal transfer of 89K occurred via a GI type IV secretion system (T4SS) encoded in 89K, at a frequency of 10(−6) transconjugants per donor. This is the first demonstration of horizontal transfer of a Gram-positive PAI mediated by a GI-type T4SS. We propose that these genetic events are important in the emergence, pathogenesis and persistence of epidemic S. suis 2 strains. Blackwell Publishing Ltd 2011-03 /pmc/articles/PMC3132442/ /pubmed/21244532 http://dx.doi.org/10.1111/j.1365-2958.2011.07553.x Text en Copyright © 2011 Blackwell Publishing Ltd http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation. |
spellingShingle | Research Articles Li, Ming Shen, Xiaodong Yan, Jinghua Han, Huiming Zheng, Beiwen Liu, Di Cheng, Hao Zhao, Yan Rao, Xiancai Wang, Changjun Tang, Jiaqi Hu, Fuquan Gao, George F GI-type T4SS-mediated horizontal transfer of the 89K pathogenicity island in epidemic Streptococcus suis serotype 2 |
title | GI-type T4SS-mediated horizontal transfer of the 89K pathogenicity island in epidemic Streptococcus suis serotype 2 |
title_full | GI-type T4SS-mediated horizontal transfer of the 89K pathogenicity island in epidemic Streptococcus suis serotype 2 |
title_fullStr | GI-type T4SS-mediated horizontal transfer of the 89K pathogenicity island in epidemic Streptococcus suis serotype 2 |
title_full_unstemmed | GI-type T4SS-mediated horizontal transfer of the 89K pathogenicity island in epidemic Streptococcus suis serotype 2 |
title_short | GI-type T4SS-mediated horizontal transfer of the 89K pathogenicity island in epidemic Streptococcus suis serotype 2 |
title_sort | gi-type t4ss-mediated horizontal transfer of the 89k pathogenicity island in epidemic streptococcus suis serotype 2 |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3132442/ https://www.ncbi.nlm.nih.gov/pubmed/21244532 http://dx.doi.org/10.1111/j.1365-2958.2011.07553.x |
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