Cargando…

GI-type T4SS-mediated horizontal transfer of the 89K pathogenicity island in epidemic Streptococcus suis serotype 2

Pathogenicity islands (PAIs), a distinct type of genomic island (GI), play important roles in the rapid adaptation and increased virulence of pathogens. 89K is a newly identified PAI in epidemic Streptococcus suis isolates that are related to the two recent large-scale outbreaks of human infection i...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Ming, Shen, Xiaodong, Yan, Jinghua, Han, Huiming, Zheng, Beiwen, Liu, Di, Cheng, Hao, Zhao, Yan, Rao, Xiancai, Wang, Changjun, Tang, Jiaqi, Hu, Fuquan, Gao, George F
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3132442/
https://www.ncbi.nlm.nih.gov/pubmed/21244532
http://dx.doi.org/10.1111/j.1365-2958.2011.07553.x
_version_ 1782207823678013440
author Li, Ming
Shen, Xiaodong
Yan, Jinghua
Han, Huiming
Zheng, Beiwen
Liu, Di
Cheng, Hao
Zhao, Yan
Rao, Xiancai
Wang, Changjun
Tang, Jiaqi
Hu, Fuquan
Gao, George F
author_facet Li, Ming
Shen, Xiaodong
Yan, Jinghua
Han, Huiming
Zheng, Beiwen
Liu, Di
Cheng, Hao
Zhao, Yan
Rao, Xiancai
Wang, Changjun
Tang, Jiaqi
Hu, Fuquan
Gao, George F
author_sort Li, Ming
collection PubMed
description Pathogenicity islands (PAIs), a distinct type of genomic island (GI), play important roles in the rapid adaptation and increased virulence of pathogens. 89K is a newly identified PAI in epidemic Streptococcus suis isolates that are related to the two recent large-scale outbreaks of human infection in China. However, its mechanism of evolution and contribution to the epidemic spread of S. suis 2 remain unknown. In this study, the potential for mobilization of 89K was evaluated, and its putative transfer mechanism was investigated. We report that 89K can spontaneously excise to form an extrachromosomal circular product. The precise excision is mediated by an 89K-borne integrase through site-specific recombination, with help from an excisionase. The 89K excision intermediate acts as a substrate for lateral transfer to non-89K S. suis 2 recipients, where it reintegrates site-specifically into the target site. The conjugal transfer of 89K occurred via a GI type IV secretion system (T4SS) encoded in 89K, at a frequency of 10(−6) transconjugants per donor. This is the first demonstration of horizontal transfer of a Gram-positive PAI mediated by a GI-type T4SS. We propose that these genetic events are important in the emergence, pathogenesis and persistence of epidemic S. suis 2 strains.
format Online
Article
Text
id pubmed-3132442
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Blackwell Publishing Ltd
record_format MEDLINE/PubMed
spelling pubmed-31324422011-07-11 GI-type T4SS-mediated horizontal transfer of the 89K pathogenicity island in epidemic Streptococcus suis serotype 2 Li, Ming Shen, Xiaodong Yan, Jinghua Han, Huiming Zheng, Beiwen Liu, Di Cheng, Hao Zhao, Yan Rao, Xiancai Wang, Changjun Tang, Jiaqi Hu, Fuquan Gao, George F Mol Microbiol Research Articles Pathogenicity islands (PAIs), a distinct type of genomic island (GI), play important roles in the rapid adaptation and increased virulence of pathogens. 89K is a newly identified PAI in epidemic Streptococcus suis isolates that are related to the two recent large-scale outbreaks of human infection in China. However, its mechanism of evolution and contribution to the epidemic spread of S. suis 2 remain unknown. In this study, the potential for mobilization of 89K was evaluated, and its putative transfer mechanism was investigated. We report that 89K can spontaneously excise to form an extrachromosomal circular product. The precise excision is mediated by an 89K-borne integrase through site-specific recombination, with help from an excisionase. The 89K excision intermediate acts as a substrate for lateral transfer to non-89K S. suis 2 recipients, where it reintegrates site-specifically into the target site. The conjugal transfer of 89K occurred via a GI type IV secretion system (T4SS) encoded in 89K, at a frequency of 10(−6) transconjugants per donor. This is the first demonstration of horizontal transfer of a Gram-positive PAI mediated by a GI-type T4SS. We propose that these genetic events are important in the emergence, pathogenesis and persistence of epidemic S. suis 2 strains. Blackwell Publishing Ltd 2011-03 /pmc/articles/PMC3132442/ /pubmed/21244532 http://dx.doi.org/10.1111/j.1365-2958.2011.07553.x Text en Copyright © 2011 Blackwell Publishing Ltd http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Research Articles
Li, Ming
Shen, Xiaodong
Yan, Jinghua
Han, Huiming
Zheng, Beiwen
Liu, Di
Cheng, Hao
Zhao, Yan
Rao, Xiancai
Wang, Changjun
Tang, Jiaqi
Hu, Fuquan
Gao, George F
GI-type T4SS-mediated horizontal transfer of the 89K pathogenicity island in epidemic Streptococcus suis serotype 2
title GI-type T4SS-mediated horizontal transfer of the 89K pathogenicity island in epidemic Streptococcus suis serotype 2
title_full GI-type T4SS-mediated horizontal transfer of the 89K pathogenicity island in epidemic Streptococcus suis serotype 2
title_fullStr GI-type T4SS-mediated horizontal transfer of the 89K pathogenicity island in epidemic Streptococcus suis serotype 2
title_full_unstemmed GI-type T4SS-mediated horizontal transfer of the 89K pathogenicity island in epidemic Streptococcus suis serotype 2
title_short GI-type T4SS-mediated horizontal transfer of the 89K pathogenicity island in epidemic Streptococcus suis serotype 2
title_sort gi-type t4ss-mediated horizontal transfer of the 89k pathogenicity island in epidemic streptococcus suis serotype 2
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3132442/
https://www.ncbi.nlm.nih.gov/pubmed/21244532
http://dx.doi.org/10.1111/j.1365-2958.2011.07553.x
work_keys_str_mv AT liming gitypet4ssmediatedhorizontaltransferofthe89kpathogenicityislandinepidemicstreptococcussuisserotype2
AT shenxiaodong gitypet4ssmediatedhorizontaltransferofthe89kpathogenicityislandinepidemicstreptococcussuisserotype2
AT yanjinghua gitypet4ssmediatedhorizontaltransferofthe89kpathogenicityislandinepidemicstreptococcussuisserotype2
AT hanhuiming gitypet4ssmediatedhorizontaltransferofthe89kpathogenicityislandinepidemicstreptococcussuisserotype2
AT zhengbeiwen gitypet4ssmediatedhorizontaltransferofthe89kpathogenicityislandinepidemicstreptococcussuisserotype2
AT liudi gitypet4ssmediatedhorizontaltransferofthe89kpathogenicityislandinepidemicstreptococcussuisserotype2
AT chenghao gitypet4ssmediatedhorizontaltransferofthe89kpathogenicityislandinepidemicstreptococcussuisserotype2
AT zhaoyan gitypet4ssmediatedhorizontaltransferofthe89kpathogenicityislandinepidemicstreptococcussuisserotype2
AT raoxiancai gitypet4ssmediatedhorizontaltransferofthe89kpathogenicityislandinepidemicstreptococcussuisserotype2
AT wangchangjun gitypet4ssmediatedhorizontaltransferofthe89kpathogenicityislandinepidemicstreptococcussuisserotype2
AT tangjiaqi gitypet4ssmediatedhorizontaltransferofthe89kpathogenicityislandinepidemicstreptococcussuisserotype2
AT hufuquan gitypet4ssmediatedhorizontaltransferofthe89kpathogenicityislandinepidemicstreptococcussuisserotype2
AT gaogeorgef gitypet4ssmediatedhorizontaltransferofthe89kpathogenicityislandinepidemicstreptococcussuisserotype2