Characterization of the Recombinant Thermostable Lipase (Pf2001) from Pyrococcus furiosus: Effects of Thioredoxin Fusion Tag and Triton X-100

In this work, the lipase from Pyrococcus furiosus encoded by ORF PF2001 was expressed with a fusion protein (thioredoxin) in Escherichia coli. The purified enzymes with the thioredoxin tag (TRX−PF2001Δ60) and without the thioredoxin tag (PF2001Δ60) were characterized, and various influences of Trito...

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Detalles Bibliográficos
Autores principales: Alquéres, Sylvia Maria Campbell, Branco, Roberta Vieira, Freire, Denise Maria Guimarães, Alves, Tito Lívio Moitinho, Martins, Orlando Bonifácio, Almeida, Rodrigo Volcan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE-Hindawi Access to Research 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3132477/
https://www.ncbi.nlm.nih.gov/pubmed/21760993
http://dx.doi.org/10.4061/2011/316939

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