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Humic Acid-Oxidizing, Nitrate-Reducing Bacteria in Agricultural Soils
This study demonstrates the prevalence, phylogenetic diversity, and physiology of nitrate-reducing microorganisms capable of utilizing reduced humic acids (HA) as electron donors in agricultural soils. Most probable number (MPN) enumeration of agricultural soils revealed large populations (10(4) to...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Microbiology
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3132874/ https://www.ncbi.nlm.nih.gov/pubmed/21750120 http://dx.doi.org/10.1128/mBio.00044-11 |
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author | Van Trump, J. Ian Wrighton, Kelly C. Thrash, J. Cameron Weber, Karrie A. Andersen, Gary L. Coates, John D. |
author_facet | Van Trump, J. Ian Wrighton, Kelly C. Thrash, J. Cameron Weber, Karrie A. Andersen, Gary L. Coates, John D. |
author_sort | Van Trump, J. Ian |
collection | PubMed |
description | This study demonstrates the prevalence, phylogenetic diversity, and physiology of nitrate-reducing microorganisms capable of utilizing reduced humic acids (HA) as electron donors in agricultural soils. Most probable number (MPN) enumeration of agricultural soils revealed large populations (10(4) to 10(6) cells g(−1) soil) of microorganisms capable of reducing nitrate while oxidizing the reduced HA analog 2,6-anthrahydroquinone disulfonate (AH(2)DS) to its corresponding quinone. Nitrate-dependent HA-oxidizing organisms isolated from agricultural soils were phylogenetically diverse and included members of the Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria. Advective up-flow columns inoculated with corn plot soil and amended with reduced HA and nitrate supported both HA oxidation and enhanced nitrate reduction relative to no-donor or oxidized HA controls. The additional electron donating capacity of reduced HA could reasonably be attributed to the oxidation of reduced functional groups. Subsequent 16S rRNA gene-based high-density oligonucleotide microarray (PhyloChip) indicated that reduced HA columns supported the development of a bacterial community enriched with members of the Acidobacteria, Firmicutes, and Betaproteobacteria relative to the no-donor control and initial inoculum. This study identifies a previously unrecognized role for HA in stimulating denitrification processes in saturated soil systems. Furthermore, this study indicates that reduced humic acids impact soil geochemistry and the indigenous bacterial community composition. |
format | Online Article Text |
id | pubmed-3132874 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | American Society of Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-31328742011-07-12 Humic Acid-Oxidizing, Nitrate-Reducing Bacteria in Agricultural Soils Van Trump, J. Ian Wrighton, Kelly C. Thrash, J. Cameron Weber, Karrie A. Andersen, Gary L. Coates, John D. mBio Research Article This study demonstrates the prevalence, phylogenetic diversity, and physiology of nitrate-reducing microorganisms capable of utilizing reduced humic acids (HA) as electron donors in agricultural soils. Most probable number (MPN) enumeration of agricultural soils revealed large populations (10(4) to 10(6) cells g(−1) soil) of microorganisms capable of reducing nitrate while oxidizing the reduced HA analog 2,6-anthrahydroquinone disulfonate (AH(2)DS) to its corresponding quinone. Nitrate-dependent HA-oxidizing organisms isolated from agricultural soils were phylogenetically diverse and included members of the Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria. Advective up-flow columns inoculated with corn plot soil and amended with reduced HA and nitrate supported both HA oxidation and enhanced nitrate reduction relative to no-donor or oxidized HA controls. The additional electron donating capacity of reduced HA could reasonably be attributed to the oxidation of reduced functional groups. Subsequent 16S rRNA gene-based high-density oligonucleotide microarray (PhyloChip) indicated that reduced HA columns supported the development of a bacterial community enriched with members of the Acidobacteria, Firmicutes, and Betaproteobacteria relative to the no-donor control and initial inoculum. This study identifies a previously unrecognized role for HA in stimulating denitrification processes in saturated soil systems. Furthermore, this study indicates that reduced humic acids impact soil geochemistry and the indigenous bacterial community composition. American Society of Microbiology 2011-07-12 /pmc/articles/PMC3132874/ /pubmed/21750120 http://dx.doi.org/10.1128/mBio.00044-11 Text en Copyright © 2011 Van Trump et al. http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License (http://creativecommons.org/licenses/by-nc-sa/3.0/) , which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Van Trump, J. Ian Wrighton, Kelly C. Thrash, J. Cameron Weber, Karrie A. Andersen, Gary L. Coates, John D. Humic Acid-Oxidizing, Nitrate-Reducing Bacteria in Agricultural Soils |
title | Humic Acid-Oxidizing, Nitrate-Reducing Bacteria in Agricultural Soils |
title_full | Humic Acid-Oxidizing, Nitrate-Reducing Bacteria in Agricultural Soils |
title_fullStr | Humic Acid-Oxidizing, Nitrate-Reducing Bacteria in Agricultural Soils |
title_full_unstemmed | Humic Acid-Oxidizing, Nitrate-Reducing Bacteria in Agricultural Soils |
title_short | Humic Acid-Oxidizing, Nitrate-Reducing Bacteria in Agricultural Soils |
title_sort | humic acid-oxidizing, nitrate-reducing bacteria in agricultural soils |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3132874/ https://www.ncbi.nlm.nih.gov/pubmed/21750120 http://dx.doi.org/10.1128/mBio.00044-11 |
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