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Genotypic Diversity and Drug Susceptibility Patterns among M. tuberculosis Complex Isolates from South-Western Ghana

OBJECTIVE: The aim of this study was to use spoligotyping and large sequence polymorphism (LSP) to study the population structure of M. tuberculosis complex (MTBC) isolates. METHODS: MTBC isolates were identified using standard biochemical procedures, IS6110 PCR, and large sequence polymorphisms. Is...

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Autores principales: Yeboah-Manu, Dorothy, Asante-Poku, Adwoa, Bodmer, Thomas, Stucki, David, Koram, Kwadwo, Bonsu, Frank, Pluschke, Gerd, Gagneux, Sebastien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3133566/
https://www.ncbi.nlm.nih.gov/pubmed/21779354
http://dx.doi.org/10.1371/journal.pone.0021906
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author Yeboah-Manu, Dorothy
Asante-Poku, Adwoa
Bodmer, Thomas
Stucki, David
Koram, Kwadwo
Bonsu, Frank
Pluschke, Gerd
Gagneux, Sebastien
author_facet Yeboah-Manu, Dorothy
Asante-Poku, Adwoa
Bodmer, Thomas
Stucki, David
Koram, Kwadwo
Bonsu, Frank
Pluschke, Gerd
Gagneux, Sebastien
author_sort Yeboah-Manu, Dorothy
collection PubMed
description OBJECTIVE: The aim of this study was to use spoligotyping and large sequence polymorphism (LSP) to study the population structure of M. tuberculosis complex (MTBC) isolates. METHODS: MTBC isolates were identified using standard biochemical procedures, IS6110 PCR, and large sequence polymorphisms. Isolates were further typed using spoligotyping, and the phenotypic drug susceptibility patterns were determined by the proportion method. RESULT: One hundred and sixty-two isolates were characterised by LSP typing. Of these, 130 (80.25%) were identified as Mycobacterium tuberculosis sensu stricto (MTBss), with the Cameroon sub-lineage being dominant (N = 59/130, 45.38%). Thirty-two (19.75%) isolates were classified as Mycobacterium africanum type 1, and of these 26 (81.25%) were identified as West-Africa I, and 6 (18.75%) as West-Africa II. Spoligotyping sub-lineages identified among the MTBss included Haarlem (N = 15, 11.53%), Ghana (N = 22, 16.92%), Beijing (4, 3.08%), EAI (4, 3.08%), Uganda I (4, 3.08%), LAM (2, 1.54%), X (N = 1, 0.77%) and S (2, 1.54%). Nine isolates had SIT numbers with no identified sub-lineages while 17 had no SIT numbers. MTBss isolates were more likely to be resistant to streptomycin (p<0.008) and to any drug resistance (p<0.03) when compared to M. africanum. CONCLUSION: This study demonstrated that overall 36.4% of TB in South-Western Ghana is caused by the Cameroon sub-lineage of MTBC and 20% by M. africanum type 1, including both the West-Africa 1 and West-Africa 2 lineages. The diversity of MTBC in Ghana should be considered when evaluating new TB vaccines.
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spelling pubmed-31335662011-07-21 Genotypic Diversity and Drug Susceptibility Patterns among M. tuberculosis Complex Isolates from South-Western Ghana Yeboah-Manu, Dorothy Asante-Poku, Adwoa Bodmer, Thomas Stucki, David Koram, Kwadwo Bonsu, Frank Pluschke, Gerd Gagneux, Sebastien PLoS One Research Article OBJECTIVE: The aim of this study was to use spoligotyping and large sequence polymorphism (LSP) to study the population structure of M. tuberculosis complex (MTBC) isolates. METHODS: MTBC isolates were identified using standard biochemical procedures, IS6110 PCR, and large sequence polymorphisms. Isolates were further typed using spoligotyping, and the phenotypic drug susceptibility patterns were determined by the proportion method. RESULT: One hundred and sixty-two isolates were characterised by LSP typing. Of these, 130 (80.25%) were identified as Mycobacterium tuberculosis sensu stricto (MTBss), with the Cameroon sub-lineage being dominant (N = 59/130, 45.38%). Thirty-two (19.75%) isolates were classified as Mycobacterium africanum type 1, and of these 26 (81.25%) were identified as West-Africa I, and 6 (18.75%) as West-Africa II. Spoligotyping sub-lineages identified among the MTBss included Haarlem (N = 15, 11.53%), Ghana (N = 22, 16.92%), Beijing (4, 3.08%), EAI (4, 3.08%), Uganda I (4, 3.08%), LAM (2, 1.54%), X (N = 1, 0.77%) and S (2, 1.54%). Nine isolates had SIT numbers with no identified sub-lineages while 17 had no SIT numbers. MTBss isolates were more likely to be resistant to streptomycin (p<0.008) and to any drug resistance (p<0.03) when compared to M. africanum. CONCLUSION: This study demonstrated that overall 36.4% of TB in South-Western Ghana is caused by the Cameroon sub-lineage of MTBC and 20% by M. africanum type 1, including both the West-Africa 1 and West-Africa 2 lineages. The diversity of MTBC in Ghana should be considered when evaluating new TB vaccines. Public Library of Science 2011-07-11 /pmc/articles/PMC3133566/ /pubmed/21779354 http://dx.doi.org/10.1371/journal.pone.0021906 Text en Yeboah-Manu et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Yeboah-Manu, Dorothy
Asante-Poku, Adwoa
Bodmer, Thomas
Stucki, David
Koram, Kwadwo
Bonsu, Frank
Pluschke, Gerd
Gagneux, Sebastien
Genotypic Diversity and Drug Susceptibility Patterns among M. tuberculosis Complex Isolates from South-Western Ghana
title Genotypic Diversity and Drug Susceptibility Patterns among M. tuberculosis Complex Isolates from South-Western Ghana
title_full Genotypic Diversity and Drug Susceptibility Patterns among M. tuberculosis Complex Isolates from South-Western Ghana
title_fullStr Genotypic Diversity and Drug Susceptibility Patterns among M. tuberculosis Complex Isolates from South-Western Ghana
title_full_unstemmed Genotypic Diversity and Drug Susceptibility Patterns among M. tuberculosis Complex Isolates from South-Western Ghana
title_short Genotypic Diversity and Drug Susceptibility Patterns among M. tuberculosis Complex Isolates from South-Western Ghana
title_sort genotypic diversity and drug susceptibility patterns among m. tuberculosis complex isolates from south-western ghana
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3133566/
https://www.ncbi.nlm.nih.gov/pubmed/21779354
http://dx.doi.org/10.1371/journal.pone.0021906
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