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Mammalian production of an isotopically enriched outer domain of the HIV-1 gp120 glycoprotein for NMR spectroscopy

NMR spectroscopic characterization of the structure or the dynamics of proteins generally requires the production of samples isotopically enriched in (15)N, (13)C, or (2)H. The bacterial expression systems currently in use to obtain isotopic enrichment, however, cannot produce a number of eukaryotic...

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Detalles Bibliográficos
Autores principales:	Sastry, Mallika, Xu, Ling, Georgiev, Ivelin S., Bewley, Carole A., Nabel, Gary J., Kwong, Peter D.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Netherlands 2011
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3133704/ https://www.ncbi.nlm.nih.gov/pubmed/21667299 http://dx.doi.org/10.1007/s10858-011-9506-4

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