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Loss of Prestin Does Not Alter the Development of Auditory Cortical Dendritic Spines

Disturbance of sensory input during development can have disastrous effects on the development of sensory cortical areas. To examine how moderate perturbations of hearing can impact the development of primary auditory cortex, we examined markers of excitatory synapses in mice who lacked prestin, a p...

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Detalles Bibliográficos
Autores principales: Bogart, L. J., Levy, A. D., Gladstone, M., Allen, P. D., Zettel, M., Ison, J. R., Luebke, A. E., Majewska, A. K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3134106/
https://www.ncbi.nlm.nih.gov/pubmed/21773053
http://dx.doi.org/10.1155/2011/305621
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author Bogart, L. J.
Levy, A. D.
Gladstone, M.
Allen, P. D.
Zettel, M.
Ison, J. R.
Luebke, A. E.
Majewska, A. K.
author_facet Bogart, L. J.
Levy, A. D.
Gladstone, M.
Allen, P. D.
Zettel, M.
Ison, J. R.
Luebke, A. E.
Majewska, A. K.
author_sort Bogart, L. J.
collection PubMed
description Disturbance of sensory input during development can have disastrous effects on the development of sensory cortical areas. To examine how moderate perturbations of hearing can impact the development of primary auditory cortex, we examined markers of excitatory synapses in mice who lacked prestin, a protein responsible for somatic electromotility of cochlear outer hair cells. While auditory brain stem responses of these mice show an approximately 40 dB increase in threshold, we found that loss of prestin produced no changes in spine density or morphological characteristics on apical dendrites of cortical layer 5 pyramidal neurons. PSD-95 immunostaining also showed no changes in overall excitatory synapse density. Surprisingly, behavioral assessments of auditory function using the acoustic startle response showed only modest changes in prestin KO animals. These results suggest that moderate developmental hearing deficits produce minor changes in the excitatory connectivity of layer 5 neurons of primary auditory cortex and surprisingly mild auditory behavioral deficits in the startle response.
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spelling pubmed-31341062011-07-19 Loss of Prestin Does Not Alter the Development of Auditory Cortical Dendritic Spines Bogart, L. J. Levy, A. D. Gladstone, M. Allen, P. D. Zettel, M. Ison, J. R. Luebke, A. E. Majewska, A. K. Neural Plast Research Article Disturbance of sensory input during development can have disastrous effects on the development of sensory cortical areas. To examine how moderate perturbations of hearing can impact the development of primary auditory cortex, we examined markers of excitatory synapses in mice who lacked prestin, a protein responsible for somatic electromotility of cochlear outer hair cells. While auditory brain stem responses of these mice show an approximately 40 dB increase in threshold, we found that loss of prestin produced no changes in spine density or morphological characteristics on apical dendrites of cortical layer 5 pyramidal neurons. PSD-95 immunostaining also showed no changes in overall excitatory synapse density. Surprisingly, behavioral assessments of auditory function using the acoustic startle response showed only modest changes in prestin KO animals. These results suggest that moderate developmental hearing deficits produce minor changes in the excitatory connectivity of layer 5 neurons of primary auditory cortex and surprisingly mild auditory behavioral deficits in the startle response. Hindawi Publishing Corporation 2011 2011-05-15 /pmc/articles/PMC3134106/ /pubmed/21773053 http://dx.doi.org/10.1155/2011/305621 Text en Copyright © 2011 L. J. Bogart et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bogart, L. J.
Levy, A. D.
Gladstone, M.
Allen, P. D.
Zettel, M.
Ison, J. R.
Luebke, A. E.
Majewska, A. K.
Loss of Prestin Does Not Alter the Development of Auditory Cortical Dendritic Spines
title Loss of Prestin Does Not Alter the Development of Auditory Cortical Dendritic Spines
title_full Loss of Prestin Does Not Alter the Development of Auditory Cortical Dendritic Spines
title_fullStr Loss of Prestin Does Not Alter the Development of Auditory Cortical Dendritic Spines
title_full_unstemmed Loss of Prestin Does Not Alter the Development of Auditory Cortical Dendritic Spines
title_short Loss of Prestin Does Not Alter the Development of Auditory Cortical Dendritic Spines
title_sort loss of prestin does not alter the development of auditory cortical dendritic spines
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3134106/
https://www.ncbi.nlm.nih.gov/pubmed/21773053
http://dx.doi.org/10.1155/2011/305621
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