Preparation of DNA/Gold Nanoparticle Encapsulated in Calcium Phosphate

Biocompatible DNA/gold nanoparticle complex with a protective calcium phosphate (CaP) coating was prepared by incubating DNA/gold nanoparticle complex coated by hyaluronic acid in SBF (simulated body fluid) with a Ca concentration above 2 mM. The CaP-coated DNA complex was revealed to have high comp...

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Detalles Bibliográficos
Autores principales: Ito, Tomoko, Ibe, Koyuki, Uchino, Tomohiro, Ohshima, Hiroyuki, Otsuka, Makoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3134889/
https://www.ncbi.nlm.nih.gov/pubmed/21773047
http://dx.doi.org/10.1155/2011/647631
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author Ito, Tomoko
Ibe, Koyuki
Uchino, Tomohiro
Ohshima, Hiroyuki
Otsuka, Makoto
author_facet Ito, Tomoko
Ibe, Koyuki
Uchino, Tomohiro
Ohshima, Hiroyuki
Otsuka, Makoto
author_sort Ito, Tomoko
collection PubMed
description Biocompatible DNA/gold nanoparticle complex with a protective calcium phosphate (CaP) coating was prepared by incubating DNA/gold nanoparticle complex coated by hyaluronic acid in SBF (simulated body fluid) with a Ca concentration above 2 mM. The CaP-coated DNA complex was revealed to have high compatibility with cells and resistance against enzymatic degradation. By immersion in acetate buffer (pH 4.5), the CaP capsule released the contained DNA complex. This CaP capsule including a DNA complex is promising as a sustained-release system of DNA complexes for gene therapy.
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spelling pubmed-31348892011-07-19 Preparation of DNA/Gold Nanoparticle Encapsulated in Calcium Phosphate Ito, Tomoko Ibe, Koyuki Uchino, Tomohiro Ohshima, Hiroyuki Otsuka, Makoto J Drug Deliv Research Article Biocompatible DNA/gold nanoparticle complex with a protective calcium phosphate (CaP) coating was prepared by incubating DNA/gold nanoparticle complex coated by hyaluronic acid in SBF (simulated body fluid) with a Ca concentration above 2 mM. The CaP-coated DNA complex was revealed to have high compatibility with cells and resistance against enzymatic degradation. By immersion in acetate buffer (pH 4.5), the CaP capsule released the contained DNA complex. This CaP capsule including a DNA complex is promising as a sustained-release system of DNA complexes for gene therapy. Hindawi Publishing Corporation 2011 2011-06-07 /pmc/articles/PMC3134889/ /pubmed/21773047 http://dx.doi.org/10.1155/2011/647631 Text en Copyright © 2011 Tomoko Ito et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ito, Tomoko
Ibe, Koyuki
Uchino, Tomohiro
Ohshima, Hiroyuki
Otsuka, Makoto
Preparation of DNA/Gold Nanoparticle Encapsulated in Calcium Phosphate
title Preparation of DNA/Gold Nanoparticle Encapsulated in Calcium Phosphate
title_full Preparation of DNA/Gold Nanoparticle Encapsulated in Calcium Phosphate
title_fullStr Preparation of DNA/Gold Nanoparticle Encapsulated in Calcium Phosphate
title_full_unstemmed Preparation of DNA/Gold Nanoparticle Encapsulated in Calcium Phosphate
title_short Preparation of DNA/Gold Nanoparticle Encapsulated in Calcium Phosphate
title_sort preparation of dna/gold nanoparticle encapsulated in calcium phosphate
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3134889/
https://www.ncbi.nlm.nih.gov/pubmed/21773047
http://dx.doi.org/10.1155/2011/647631
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